Sulfo-EGS (ethylene glycol bis(sulfosuccinimidyl succinate))

Citations & References (7)

Thermo Scientific™

Sulfo-EGS (ethylene glycol bis(sulfosuccinimidyl succinate))

Thermo Scientific Pierce Sulfo-EGSは、12-原子スペーサーアームの周囲にアミン反応性sulfo-NHS-エステル末端を含む水溶性クロスリンカーで、pH 8.5でヒドロキシルアミンで処理して開裂できます。Sulfo-EGSの特長：•反応基：Sulfo-NHSエステル（両端）•詳細を見る

製品番号（カタログ番号）	数量
21566	50 mg

製品番号（カタログ番号） 21566

価格（JPY）

42,100

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50 mg

一括またはカスタム形式をリクエストする

Thermo Scientific Pierce Sulfo-EGSは、12-原子スペーサーアームの周囲にアミン反応性sulfo-NHS-エステル末端を含む水溶性クロスリンカーで、pH 8.5でヒドロキシルアミンで処理して開裂できます。

Sulfo-EGSの特長：

•反応基：Sulfo-NHSエステル（両端）
• 反応性：アミノ基（第一級アミン）
• 水溶性、EGSと比較
• 膜非透過性、細胞表面の標識が可能
• 形成した架橋は37℃で3～6時間ヒドロキシルアミンを使用して、pH 8.5で可逆化可能
• ラクトースデヒドロゲナーゼはEGSで可逆的に架橋した後もその活動の60%を維持します

製品リファレンス：
架橋剤アプリケーションガイド - この製品に関する最近の参考文献を検索します

関連製品
Sulfo-EGS（エチレングリコールビス（sulfoスクシンイミジルスクシネート））

研究用途にのみご使用ください。診断目的には使用できません。

細胞透過性No

概要Sulfo-EGS

形状Powder

標識法化学的標識

分子量660.45

PEG化No

製品ラインPierce

数量50 mg

反応性部分スルホ-NHSエステル

出荷条件Ambient

スペーサーアーム長16.1 Å

水溶性Yes

化学反応性Amine-Amine

CleavableBy Hydroxylamine

クロスリンカータイプホモ二官能性

フォーマットStandard

製品タイプ架橋剤

スペーサー切断可能、長

Unit SizeEach

組成および保存条件

受け取り後、-20℃で乾燥した場所に保存。製品は室温で出荷されます。

よくあるご質問（FAQ）

Can you provide the shelf-life for Sulfo-EGS (ethylene glycol bis(sulfosuccinimidyl succinate))?

Sulfo-EGS (ethylene glycol bis(sulfosuccinimidyl succinate)) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

引用および参考文献 (7)

引用および参考文献

Abstract

Role of TRIM5a RING domain E3 ubiquitin ligase activity in capsid disassembly, reverse transcription blockade, and restriction of simian immunodeficiency virus.

Authors:Kim J, Tipper C, Sodroski J

Journal:J Virol

PubMed ID:21680520

'The mammalian tripartite motif protein, TRIM5a, recognizes retroviral capsids entering the cytoplasm and blocks virus infection. Depending on the particular TRIM5a protein and retrovirus, complete disruption of the TRIM5a RING domain decreases virus-restricting activity to various degrees. TRIM5a exhibits RING domain-dependent E3 ubiquitin ligase activity, but the specific role of ... More

A novel C-terminal mutation resulting in constitutive activation of the Listeria monocytogenes central virulence regulatory factor PrfA.

Authors:Xayarath B, Smart JI, Mueller KJ, Freitag NE

Journal:Microbiology

PubMed ID:21835879

'The environmental bacterium Listeria monocytogenes survives and replicates in a variety of diverse ecological niches that range from the soil to the cytosol of infected mammalian cells. The ability of L. monocytogenes to replicate within an infected host requires the expression of a number of secreted bacterial gene products whose ... More

Raf kinase inhibitor protein (RKIP) dimer formation controls its target switch from Raf1 to G protein-coupled receptor kinase (GRK) 2.

Authors:Deiss K, Kisker C, Lohse MJ, Lorenz K

Journal:J Biol Chem

PubMed ID:22610096

'Proteins controlling cellular networks have evolved distinct mechanisms to ensure specificity in protein-protein interactions. Raf kinase inhibitor protein (RKIP) is a multifaceted kinase modulator, but it is not well understood how this small protein (21 kDa) can coordinate its diverse signaling functions. Raf1 and G protein-coupled receptor kinase (GRK) 2 ... More

Syk-dependent phosphorylation of CLEC-2: a novel mechanism of hem-immunoreceptor tyrosine-based activation motif signaling.

Authors:Séverin S, Pollitt AY, Navarro-Nuñez L, Nash CA, Mourão-Sá D, Eble JA, Senis YA, Watson SP

Journal:J Biol Chem

PubMed ID:21098033

The C-type lectin-like receptor CLEC-2 signals via phosphorylation of a single cytoplasmic YXXL sequence known as a hem-immunoreceptor tyrosine-based activation motif (hemITAM). In this study, we show that phosphorylation of CLEC-2 by the snake toxin rhodocytin is abolished in the absence of the tyrosine kinase Syk but is not altered ... More

Structural basis of molecular recognition of the Leishmania small hydrophilic endoplasmic reticulum-associated protein (SHERP) at membrane surfaces.

Authors:Moore B, Miles AJ, Guerra-Giraldez C, Simpson P, Iwata M, Wallace BA, Matthews SJ, Smith DF, Brown KA

Journal:J Biol Chem

PubMed ID:21106528

The 57-residue small hydrophilic endoplasmic reticulum-associated protein (SHERP) shows highly specific, stage-regulated expression in the non-replicative vector-transmitted stages of the kinetoplastid parasite, Leishmania major, the causative agent of human cutaneous leishmaniasis. Previous studies have demonstrated that SHERP localizes as a peripheral membrane protein on the cytosolic face of the endoplasmic ... More

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