What are the storage conditions for CultureOne Supplement? Can I store it at 4 degrees C and for how long? Can I aliquot unused product and freeze it at -20 degrees C?
We recommend that you thaw the CultureOne Supplement and then aliquot it out for working stocks and re-freeze the aliquots. We have found that the product can undergo up to 3 freeze-thaws without loss of activity. CultureOne-supplemented media is good for 2 weeks stored at 4 degrees C in the dark.
I am using the B-27 Plus Neuronal Culture System. What do I do if my neuronal cells are not attaching or are clumped?
In primary neuronal cultures, this is typically a function of uneven coating or if neurons are plated at too high of a density. In NSC differentiation experiments, this could be due to overproliferation of neural progenitor cells. Differentiating with the B-27 Plus Neuronal Culture System and CultureOne Supplement can eliminate more than 75% of neural progenitor cells and the clumps they form. Please see the CultureOne Supplement User Guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0016204_CultureOneSupplement_UG.pdf) for more information.
I am planning to use the B-27 Plus Neuronal Culture System. How can I control the number of glial cells in my neuronal culture?
Add CultureOne Supplement at day 0 with the B-27 Plus Neuronal Culture System to fully suppress both astrocytes and oligodendrocytes with no detrimental effect on neurons. Delaying the addition of CultureOne Supplement to later time points results in increasing levels of astrocytes. Refer to the CultureOne Supplement User Guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0016204_CultureOneSupplement_UG.pdf) for more information.