Genexus™ Library Strips 1 and 2-HD

The Ion Torrent Genexus Library Strips 1 and 2-HD kit for Ion AmpliSeq HD-based panels provides eight each of the詳細を見る

製品番号（カタログ番号）	数量
A40255	32 Reactions

製品番号（カタログ番号） A40255

価格（JPY）

809,300

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32 Reactions

The Ion Torrent Genexus Library Strips 1 and 2-HD kit for Ion AmpliSeq HD-based panels provides eight each of the strips required to perform automated library preparation using the Ion Torrent Genexus Integrated Sequencer. This kit, combined with Ion Torrent Genexus Barcodes 1-32 HD, supports flexible, fully automated preparation and normalization of up to 32 single-pool Ion AmpliSeq HD libraries.

As part of the Genexus Integrated Sequencer workflow, the Genexus Library Strips 1 and 2-HD kit:
• Automates library preparation for 200–400 base-read Ion AmpliSeq HD libraries
• Supports intake variability of clinical research samples in a cost-effective manner
• Maximizes flexibility with the ability prepare 1 to 32 single-pool libraries per run
• Enables parallel processing of up to four compatible assays in a single run
• Delivers fast and simple instrument setup, with less than five minutes of hands-on time

The Ion Torrent Genexus Integrated Sequencer automates library preparation, sequencing, analysis, and reporting in a hands-off, walkaway workflow to deliver results in a single day. The innovative, multilane sequencing chip is designed to enable cost-effective processing of 1 to 32 single pool libraries.

研究用途にのみご使用ください。診断目的には使用できません。

使用対象（アプリケーション）配列

使用対象 (装置)Genexus™ Integrated Sequencer

ライブラリTargeted Sequencing Library

反応数32

製品ラインGenexus

製品タイプLibrary Strips 1 and 2-HD

数量32 Reactions

サンプルタイプDNA, RNA

出荷条件Approved for shipment on Wet or Dry Ice

Sequencing TypeGenome, DNA, and RNA Sequencing

Unit SizeEach

組成および保存条件

• Genexus Strip 2-HD, store at -10 to -30°C
• Genexus Strip 1, store at 2–8°C

よくあるご質問（FAQ）

How many samples of Oncomine Precision Assay GX can I run on each lane of the GX5 Chip?

The capacity of the GX5 Chip varies depending on the sample type:
- For plasma cfTNA samples, the workflow is configured to accommodate 1 sample per GX5 Chip lane (or 4 samples on a single GX5 Chip).
- For FFPE DNA+RNA samples, the workflow is configured to accommodate 4 samples per GX5 Chip lane.
- For FFPE DNA-only or RNA-only samples, the workflow is configured to accommodate 8 samples per GX5 Chip lane.

How many sequencing reactions can I perform with Oncomine Precision Assay GX (Cat. No. A44350)?

The maximum number of samples that can be analyzed per kit depends upon the type of analysis that you want to perform. Each kit provides the targeted pancancer panel and library reagents sufficient to perform up to 32 sequencing reactions on the Genexus Integrated Sequencer.

- Simultaneous DNA- and RNA-based variant analysis for up to 16 FFPE tissue samples (one DNA reaction and one RNA reaction per sample)
- DNA-only or RNA-only variant analysis for up to 32 FFPE tissue samples
- Cell-free TNA (cfTNA) variant analysis (combined DNA and RNA) for up to 32 plasma samples

How much of each sample do I need for the Oncomine Precision Assay GX?

For solid tumor samples, the optimal input amount is 10 ng with validated input in the range of 5-15 ng. For liquid biopsy samples, the optimal input amount is 20 ng with validated input in the range of 5-40 ng.

For optimal results, load 20 µL of input with a concentration of ≥0.67 ng/µL for FFPE DNA and FFPE RNA samples and 20 µL of input with a concentration of ≥1.33 ng/µL for cfTNA samples. If the concentration of the sample is lower than optimal, use as much input as possible for each reaction.

What controls should I use with Oncomine Precision Assay GX?

Here are the various controls we recommend depending upon the starting material:

FFPE DNA samples
- Structural Multiplex Reference Standard (FFPE), Cat. No. HD789 (Horizon™)
- EGFR Gene-Specific Multiplex Reference Standard (FFPE) 5% AF, Cat. No. HD300 (Horizon™)
- KRAS Gene-Specific Multiplex Reference Standard (FFPE), Cat. No. HD301 (Horizon™)
- Seraseq® Lung & Brain CNV Mix, + 3 copies, Cat. No. 0710-0414 (SeraCare®)
- Seraseq® Breast CNV Mix, + 6 copies, Cat. No. 0710-0412 (SeraCare®)

FFPE RNA samples
- Seraseq® FFPE Tumor Fusion RNA v4 Reference Material, Cat. No. 0710-0496 (SeraCare®)
- Seraseq® FFPE NTRK Fusion RNA Reference Material, Cat. No. 0710-1031 (SeraCare®)

cfTNA samples
- Multiplex I cfDNA Reference Standard Set, Cat. No. HD780 (Horizon™)

Which sample types can I use with Oncomine Precision Assay GX?

Oncomine Precision Assay GX is compatible with solid tumor samples (FFPE DNA or FFPE RNA) as well as liquid biopsy samples (cfTNA).

View all Genexus™ Library Strips 1 and 2-HD FAQs