CoroNa™ Green, AM, cell permeant - Special Packaging

Citations & References (25)

Invitrogen™

CoroNa™ Green, AM, cell permeant - Special Packaging

CoroNaグリーン色素は、Na+と結合すると緑色蛍光発光強度が増加し、波長の変化が少ないナトリウムイオンインジケータです。他のナトリウムインジケータと同様に、SBFI（カタログ番号S1262、S1263、S1264）やナトリウムグリーン色素（カタログ番号S6900詳細を見る

製品番号（カタログ番号）	数量
C36676	20 x 50 μg

製品番号（カタログ番号） C36676

価格（JPY）

148,700

お問い合わせください ›

20 x 50 μg

CoroNaグリーン色素は、Na⁺と結合すると緑色蛍光発光強度が増加し、波長の変化が少ないナトリウムイオンインジケータです。他のナトリウムインジケータと同様に、SBFI（カタログ番号S1262、S1263、S1264）やナトリウムグリーン色素（カタログ番号S6900、S6901）、CoroNaグリーンインジケータは、他の一価カチオンの生理的濃度の存在下でNa⁺濃度の空間的および時間的な分解能を可能にします。CoronaグリーンAM色素は、四酢酸ナトリウムグリーンよりも効率的に細胞にロードされ、CoroNa Greenインジケータは、より広い範囲のNa^Na+濃度に応答します。Na⁺結合型の約492/516 nmの吸収／最大発光値で、CoroNaグリーン色素は、フルオレセインを検出できるあらゆる機器で検出することが可能です。Coronaグリーン色素は、細胞不透過型（カタログ番号 C36675）で入手可能です。

カルシウム、カリウム、pH、膜電位インジケータなどのイオンインジケータの詳細については、こちらを参照してください›

研究用にのみ使用できます。診断用には使用いただけません。

検出法蛍光

染色剤タイプナトリウムインジケータ

数量20 x 50 μg

出荷条件室温

使用対象（アプリケーション）細胞の生存率と増殖

使用対象 (装置)蛍光顕微鏡

製品ラインCoroNa

製品タイプナトリウムイオンインジケーター

Unit SizeEach

組成および保存条件

フリーザー（-5℃～-30℃）に保存し、遮光してください。

よくあるご質問（FAQ）

Why don't I see a significant change in signal for my live-cell fluorescent indicator dye?

Regardless of the type of live-cell indicator dye (e.g., calcium indicators, pH indicator, metal ion indicators), make sure there is no serum during the loading step, which can prematurely cleave dyes with AM esters and bind dyes non-specifically. Always optimize the dye concentration and staining time with a positive control before you run your test samples, to give the best signal-to-background. Always run a positive control with a buffer containing free ions of known concentration and an ionophore to open pores to those ions (for instance, for calcium indicators like Fluo-4 AM, this would include a buffer with added calcium combined with calcimycin, or for pH indicators, buffers of different pHs combined with nigericin). Reactive oxygen indicators, such as CellROX Green or H2DCFDA would require a cellular reactive oxygen species (ROS) stimulant as a positive control, such as menadione. Finally, make sure your imaging system has a sensitive detector. Plate readers, for instance, have much lower detector efficiency over background, compared to microscopy or flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (25)

引用および参考文献

Abstract

Arabidopsis fatty acid desaturase FAD2 is required for salt tolerance during seed germination and early seedling growth.

Authors:Zhang J, Liu H, Sun J, Li B, Zhu Q, Chen S, Zhang H,

Journal:PLoS One

PubMed ID:22279586

'Fatty acid desaturases play important role in plant responses to abiotic stresses. However, their exact function in plant resistance to salt stress is unknown. In this work, we provide the evidence that FAD2, an endoplasmic reticulum localized ?-6 desaturase, is required for salt tolerance in Arabidopsis. Using vacuolar and plasma ... More

Diacylglycerol analogues activate second messenger-operated calcium channels exhibiting TRPC-like properties in cortical neurons.

Authors:Tu P, Kunert-Keil C, Lucke S, Brinkmeier H, Bouron A,

Journal:J Neurochem

PubMed ID:19094061

'The lipid diacylglycerol (DAG) analogue 1-oleoyl-2-acetyl-sn-glycerol (OAG) was used to verify the existence of DAG-sensitive channels in cortical neurons dissociated from E13 mouse embryos. Calcium imaging experiments showed that OAG increased the cytosolic concentration of Ca(2+) ([Ca(2+)]i) in nearly 35% of the KCl-responsive cells. These Ca(2+) responses disappeared in a ... More

Membrane phosphatidylserine distribution as a non-apoptotic signalling mechanism in lymphocytes.

Authors:Elliott JI, Surprenant A, Marelli-Berg FM, Cooper JC, Cassady-Cain RL, Wooding C, Linton K, Alexander DR, Higgins CF

Journal:Nat Cell Biol

PubMed ID:16025105

'Phosphatidylserine (PS) exposure is normally associated with apoptosis and the removal of dying cells. We observed that PS is exposed constitutively at high levels on T lymphocytes that express low levels of the transmembrane tyrosine phosphatase CD45RB. CD45 was shown to be a negative regulator of PS translocation in response ... More

Loss of halophytism by interference with SOS1 expression.

Authors:Oh DH, Leidi E, Zhang Q, Hwang SM, Li Y, Quintero FJ, Jiang X, D'Urzo MP, Lee SY, Zhao Y, Bahk JD, Bressan RA, Yun DJ, Pardo JM, Bohnert HJ,

Journal:Plant Physiol

PubMed ID:19571313

'The contribution of SOS1 (for Salt Overly Sensitive 1), encoding a sodium/proton antiporter, to plant salinity tolerance was analyzed in wild-type and RNA interference (RNAi) lines of the halophytic Arabidopsis (Arabidopsis thaliana)-relative Thellungiella salsuginea. Under all conditions, SOS1 mRNA abundance was higher in Thellungiella than in Arabidopsis. Ectopic expression of ... More

Cationic gradient reversal and cytoskeleton-independent volume regulatory pathways define an early stage of apoptosis.

Authors:Bortner CD, Sifre MI, Cidlowski JA,

Journal:J Biol Chem

PubMed ID:18187415

'Cell shrinkage, or apoptotic volume decrease (AVD), is a ubiquitous characteristic of programmed cell death that is independent of the death stimulus and occurs in all examples of apoptosis. Here we distinguished two specific stages of AVD based on cell size and a unique early reversal of intracellular ions that ... More

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