Uncoupling of the base excision and nucleotide incision repair pathways reveals their respective biological roles.
Authors:Ishchenko AA, Deprez E, Maksimenko A, Brochon JC, Tauc P, Saparbaev MK
Journal:Proc Natl Acad Sci U S A
PubMed ID:16473948
'The multifunctional DNA repair enzymes apurinic/apyrimidinic (AP) endonucleases cleave DNA at AP sites and 3''-blocking moieties generated by DNA glycosylases in the base excision repair pathway. Alternatively, in the nucleotide incision repair (NIR) pathway, the same AP endonucleases incise DNA 5'' of a number of oxidatively damaged bases. At present, ... More
Fluorescence imaging study of extracellular zinc at the hippocampal mossy fiber synapse.
Authors:Bastian C, Li YV
Journal:Neurosci Lett
PubMed ID:17485170
'Although synaptically released, vesicular Zn(2+) has been proposed to play a neuromodulatory or neuronal signaling role at the mossy fiber-CA3 synapse, Zn(2+) release remains controversial, especially when detected using fluorescent imaging. In the present study, we investigated synaptically released Zn(2+) at the mossy fiber (MF) synapse in rat hippocampal slices ... More
Determining zinc with commonly used calcium and zinc fluorescent indicators, a question on calcium signals.
Authors:Martin JL, Stork CJ, Li YV
Journal:Cell Calcium
PubMed ID:16764924
'Investigations into the roles of Ca(2+) and Zn(2+) in cell biology have been facilitated by the development of sensitive fluorometric probes that have enabled the measurement of Ca(2+) or Zn(2+) in both extracellular and intracellular environments. It is critical to be aware of the specificity and relative selectivity of a ... More
Integration of multianalyte sensing functions on a capillary-assembled microchip: simultaneous determination of ion concentrations and enzymatic activities by a "drop-and-sip" technique.
Authors:Henares TG, Takaishi M, Yoshida N, Terabe S, Mizutani F, Sekizawa R, Hisamoto H
Journal:Anal Chem
PubMed ID:17263315
'A general and simple implementation of simultaneous multiparametric sensing in a single microchip is presented by using a capillary-assembled microchip (CAs-CHIP) integrated with the plural different reagent-release capillaries (RRCs), acting as various biochemical sensors. A novel "drop-and-sip" technique of fluid handling is performed with a microliter droplet of a model ... More
Dual nanomolar and picomolar Zn(II) binding properties of metallothionein.
Authors:Krezel A, Maret W,
Journal:J Am Chem Soc
PubMed ID:17696343
'Each of the seven Zn(II) ions in the Zn(3)S(9) and Zn(4)S(11) clusters of human metallothionein is in a tetrathiolate coordination environment. Yet analysis of Zn(II) association with thionein, the apoprotein, and analysis of Zn(II) dissociation from metallothionein using the fluorescent chelating agents FluoZin-3 and RhodZin-3 reveal at least three classes ... More