FUN™ 1 Cell Stain

Citations & References (47)

Invitrogen™

FUN™ 1 Cell Stain

FUN™ 1は、酵母や真菌のためのユニークな2色の蛍光性生存率プローブです。FUN™ 1染色剤は、さまざまな種類の細胞に受動的に拡散し、最初は細胞質をびまん性に分布した緑色蛍光で染色します。しかし、いくつかの一般的な酵母および真菌類では、生細胞で色素を処理すると詳細を見る

製品番号（カタログ番号）	数量
F7030	100 μL

製品番号（カタログ番号） F7030

価格（JPY）

61,400

お問い合わせください ›

100 μL

FUN™ 1は、酵母や真菌のためのユニークな2色の蛍光性生存率プローブです。FUN™ 1染色剤は、さまざまな種類の細胞に受動的に拡散し、最初は細胞質をびまん性に分布した緑色蛍光で染色します。しかし、いくつかの一般的な酵母および真菌類では、生細胞で色素を処理すると、緑色細胞質蛍光の減少を伴う著しい赤色蛍光を示すコンパクトな形態をした明確な液胞構造が形成されます。液胞内構造の形成には、細胞膜の完全性と代謝能力の両方が必要です。死細胞は、識別可能な赤い構造を持たない明るい黄緑色蛍光を発します。

カルシウム、カリウム、pH、膜電位インジケータなどのイオンインジケータの詳細については、こちらを参照してください›

研究用にのみ使用できます。診断用には使用いただけません。

細胞タイプ真菌細胞、酵母細胞

濃度10 mM

検出法蛍光

染色剤タイプその他の標識または色素

フォーマットチューブ、スライド

数量100 μL

出荷条件室温

使用対象 (装置)蛍光顕微鏡, フローサイトメーター

製品ラインFUN

製品タイプ染色

Unit SizeEach

組成および保存条件

フリーザー（-5℃～-30℃）に保存し、遮光してください。

よくあるご質問（FAQ）

Can FUN 1 stained cells be examined by flow cytometry?

Yes. Use a 488 nm laser line and standard FITC and PE channels for two-color detection of green (dead/metabolically inactive cells) and red (live, metabolically active cells) emission.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Live, metabolically active fungi transport FUN 1 into vacuoles to give a red-shifted fluorescence versus green/yellow fluorescence in the nucleus and cytoplasm of dead or metabolically-inactive cells. Is this a reliable indicator of fungal viability?

No. FUN 1 accumulates into vacuoles by an unknown transport pathway, but any mutants/ recombinant cells or experimental treatments that result in a deficiency or block in vesicle-mediate transport into vacuoles may result in cells that do not have red vacuoles, even though the cells are live and metabolically active. For more information see J Microbiol Methods 78:208 (2009).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (47)

引用および参考文献

Abstract

SOD2 functions downstream of Sch9 to extend longevity in yeast.

Authors:Fabrizio P, Liou LL, Moy VN, Diaspro A, Valentine JS, Gralla EB, Longo VD

Journal:Genetics

PubMed ID:12586694

'Signal transduction pathways inactivated during periods of starvation are implicated in the regulation of longevity in organisms ranging from yeast to mammals, but the mechanisms responsible for life-span extension are poorly understood. Chronological life-span extension in S. cerevisiae cyr1 and sch9 mutants is mediated by the stress-resistance proteins Msn2/Msn4 and ... More

Early events in macrophage killing of Aspergillus fumigatus conidia: new flow cytometric viability assay.

Authors:Marr KA, Koudadoust M, Black M, Balajee SA

Journal:Clin Diagn Lab Immunol

PubMed ID:11687470

'Detailed investigations of macrophage phagocytosis and killing of Aspergillus fumigatus conidia have been limited by technical difficulties in quantifying fungal uptake and viability. In order to study early events in cell pathogen ingestion and killing, we developed a new flow cytometry assay that utilizes the fungus-specific viability dye FUN-1. Metabolically ... More

Human immature dendritic cells efficiently bind and take up secretory IgA without the induction of maturation.

Authors:Heystek HC, Moulon C, Woltman AM, Garonne P, van Kooten C

Journal:J Immunol

PubMed ID:11751952

'Immature dendritic cells (DC) reside in peripheral tissues, where they pick up and process incoming pathogens via scavenger receptors or FcR such as FcgammaR and FcepsilonR. At mucosal surfaces, IgA is the main Ig to protect the body from incoming pathogens. In addition, DC are present in high numbers at ... More

In vitro growth and analysis of Candida biofilms.

Authors:Chandra J, Mukherjee PK, Ghannoum MA,

Journal:Nat Protoc

PubMed ID:19180075

'Evaluation of fungal biofilm formation can be performed using several techniques. In this protocol, we describe methods used to form Candida biofilms on three different medical device substrates (denture strips, catheter disks and contact lenses) to quantify them and to evaluate their architecture and drug susceptibility. Biofilm formation involves adhesion ... More

Viruses activate a genetically conserved cell death pathway in a unicellular organism.

Authors:Ivanovska I, Hardwick JM

Journal:J Cell Biol

PubMed ID:16061692

'Given the importance of apoptosis in the pathogenesis of virus infections in mammals, we investigated the possibility that unicellular organisms also respond to viral pathogens by activating programmed cell death. The M1 and M2 killer viruses of Saccharomyces cerevisiae encode pore-forming toxins that were assumed to kill uninfected yeast cells ... More

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