Phen Green™ SK, Diacetate
Phen Green™ SK, Diacetate
Invitrogen™

Phen Green™ SK, Diacetate

緑色蛍光重金属インジケータPhen Green™ SK、ジアセタートは、Cu2+、Cu+、Fe2+、Hg2+、Pb2+、Cd2+詳細を見る
製品番号(カタログ番号)数量
P143131 mg
製品番号(カタログ番号) P14313
価格(JPY)
70,700
Each
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数量:
1 mg
緑色蛍光重金属インジケータPhen Green™ SK、ジアセタートは、Cu2+、Cu+、Fe2+、Hg2+、Pb2+、Cd2+、Zn2+、Ni2+など、さまざまなイオンの検出に使用できます。

カルシウム、カリウム、pH、膜電位インジケータなど、イオンインジケータの詳細を見る›

研究用にのみ使用できます。診断用には使用いただけません。
仕様
検出法蛍光
染色剤タイプ亜鉛インジケータ、鉄インジケータ、ニッケルインジケータ、水銀インジケータ、カドミウムインジケータ、銅インジケータ、鉛インジケータ
数量1 mg
出荷条件室温
使用対象 (装置)蛍光顕微鏡, マイクロプレートリーダー
製品ラインPhenグリーン
製品タイプ二酢酸
Unit SizeEach
組成および保存条件
フリーザー(-5℃~-30℃)に保存。

引用および参考文献 (28)

引用および参考文献
Abstract
Determination of the chelatable iron pool of single intact cells by laser scanning microscopy.
Authors:Petrat F, de Groot H, Rauen U
Journal:Arch Biochem Biophys
PubMed ID:10729192
'We have previously established a method of detecting intracellular chelatable iron in viable cells based on digital fluorescence microscopy. To quantify cellular chelatable iron, it was crucial to determine the intracellular indicator concentration. In the present study, we therefore adapted the method to confocal laser scanning microscopy, which should allow ... More
Cold-induced apoptosis of hepatocytes: mitochondrial permeability transition triggered by nonmitochondrial chelatable iron.
Authors:Rauen U, Kerkweg U, Weisheit D, Petrat F, Sustmann R, de Groot H
Journal:Free Radic Biol Med
PubMed ID:14680689
'We previously described that the cold-induced apoptosis of cultured hepatocytes is mediated by an increase in the cellular chelatable iron pool. We here set out to assess whether a mitochondrial permeability transition (MPT) is involved in cold-induced apoptosis. When cultured hepatocytes were rewarmed after 18 h of cold (4 degrees ... More
Copper transport across pea thylakoid membranes.
Authors:Shingles R, Wimmers LE, McCarty RE
Journal:Plant Physiol
PubMed ID:15122011
'The initial rate of Cu2+ movement across the thylakoid membrane of pea (Pisum sativum) chloroplasts was directly measured by stopped-flow spectrofluorometry using membranes loaded with the Cu(2+)-sensitive fluorophore Phen Green SK. Cu2+ transport was rapid, reaching completion within 0.5 s. The initial rate of uptake was dependent upon Cu2+ concentration ... More
Determination of the chelatable iron pool of isolated rat hepatocytes by digital fluorescence microscopy using the fluorescent probe, phen green SK.
Authors:Petrat F, Rauen U, de Groot H
Journal:Hepatology
PubMed ID:10094962
'The intracellular pool of chelatable iron is considered to be a decisive pathogenetic factor for various kinds of cell injury. We therefore set about establishing a method of detecting chelatable iron in isolated hepatocytes based on digital fluorescence microscopy. The fluorescence of hepatocytes loaded with the fluorescent metal indicators, phen ... More
The relationship between intracellular free iron and cell injury in cultured neurons, astrocytes, and oligodendrocytes.
Authors:Kress GJ, Dineley KE, Reynolds IJ
Journal:J Neurosci
PubMed ID:12122047
'Iron is an essential element for cells but may also be an important cytotoxin. However, very little is known about iron transport, redox status, or toxicity specifically inside cells. In this study, we exploited the sensitivity of fura-2 to quenching by ferrous iron (Fe(2+)) to detect intracellular free iron ([Fe(2+)](i)) ... More