Patch-clamp detection of macromolecular translocation along nuclear pores.
Authors:Bustamante JO, Varanda WA
Journal:Braz J Med Biol Res
PubMed ID:9698781
'The present paper reviews the application of patch-clamp principles to the detection and measurement of macromolecular translocation along the nuclear pores. We demonstrate that the tight-seal ''gigaseal'' between the pipette tip and the nuclear membrane is possible in the presence of fully operational nuclear pores. We show that the ability ... More
Extracellular Ca2+ depletion contributes to fast activity-dependent modulation of synaptic transmission in the brain.
Authors:Rusakov DA, Fine A
Journal:Neuron
PubMed ID:12546823
'Synaptic activation is associated with rapid changes in intracellular Ca(2+), while the extracellular Ca(2+) level is generally assumed to be constant. Here, using a novel optical method to measure changes in extracellular Ca(2+) at high spatial and temporal resolution, we find that brief trains of synaptic transmission in hippocampal area ... More
Rhod-5N as a fluorescent molecular sensor of cadmium(II) ion.
Authors:Soibinet M, Souchon V, Leray I, Valeur B,
Journal:J Fluoresc
PubMed ID:18488146
The photophysical and complexing properties of Rhod-5N (commercially available) in MOPS buffer are reported. This fluorescent molecular sensor consists of a BAPTA chelating moiety bound to a rhodamine fluorophore. Its fluorescence quantum yield is low and a drastic enhancement of fluorescence intensity upon cation binding was observed. Special attention was ... More
Measurement of limestone biodeterioration using the Ca2+ binding fluorochrome Rhod-5N.
Authors:McNamara CJ, Perry TD, Bearce K, Hernandez-Duque G, Mitchell R
Journal:J Microbiol Methods
PubMed ID:15722151
Limestone and marble have been used extensively in the construction of modern and historic buildings. Microbial colonization and growth on these stone structures is common. Microbial deterioration of stone has been assessed by measuring Ca2+ released from the stone, using ion selective electrodes and titration with EDTA. In this study, ... More
Low-affinity Ca2+ indicators compared in measurements of skeletal muscle Ca2+ transients.
Authors:Hollingworth S, Gee KR, Baylor SM,
Journal:Biophys J
PubMed ID:19804716
The low-affinity fluorescent Ca(2+) indicators OGB-5N, Fluo-5N, fura-5N, Rhod-5N, and Mag-fluo-4 were evaluated for their ability to accurately track the kinetics of the spatially averaged free Ca(2+) transient (Delta[Ca(2+)]) in skeletal muscle. Frog single fibers were injected with one of the above indicators and, usually, furaptra (previously shown to rapidly ... More