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Invitrogen
TAP Tag Monoclonal Antibody (22F12-1E3)
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TAP Tag Antibody (MA1-108) in WB
Western blot was performed using Anti-TAP Tag Monoclonal Antibody (22F12-1E3) (Product # MA1-108) by loading whole cell extracts of untransfected and transiently transfected HEK-293E lysates: untransfected, 50 µg (Lane 1), empty vector control, 50 µg (Lane 2), H3-TAP, 50 µg (Lane 3), H3-TAP, 25 µg (Lane 4), H3-TAP, 12.5 µg (Lane 5), H3-TAP, 6.25 µg (Lane 6), p65-TAP, 50 µg (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Pro... View More
Product Details
MA1-108
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Published species
Tag
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
22F12-1E3
Immunogen
KLH conjugated peptide representing the C-terminus of the TAP construct after TEV cleavage
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA, 30% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-108 detects TAP in Western blot applications. The MA1-108 immunogen is a KLH-conjugated peptide representing the C-terminus of the TAP construct after TEV cleavage. TAP adds about 20 kDa to the size of the protein.
Target Information
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tandem affinity purification (TAP) is an affinity purification method for isolation of TAP-tagged proteins together with associated proteins. The protocol involves the fusion of the "TAP tag" (typically a calmodulin binding peptide (CBP), a tobacco etch virus protease (TEV protease) cleavage site and Protein A) to the protein of interest. The TAP technique is useful in analyzing in vivo interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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