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Invitrogen
TAP Tag Monoclonal Antibody (22F12-1E3), HRP
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TAP Tag Antibody (MA1-108-HRP) in WB
Western blot analysis of the TAP tag was performed by loading the indicated amounts of GST-TAP-tag E. coli lysate and 10 µL of PageRuler Plus Prestained Protein Ladder (Product # 26619) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with StartingBlock T20 Blocking Buffer (Product # 37543) for at least 1 hour. The membrane was probed with an HRP-conjugated TAP tag monoclonal antibody (Product # MA1-108-HRP) at a dilution of 1:1000 overnight at 4°C on a rocking platform, and washed in TBS-0... View More
Product Details
MA1-108-HRP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Published species
Tag
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
22F12-1E3
Immunogen
KLH conjugated peptide representing the C-terminus of the TAP construct after TEV cleavage
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.07% Kathon
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1108-HRP detects the Tev cleavage peptide sequence. MA1108-HRP has been successfully used for direct detection of TAP in Western Blot.
Target Information
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tandem affinity purification (TAP) is an affinity purification method for isolation of TAP-tagged proteins together with associated proteins. The protocol involves the fusion of the "TAP tag" (typically a calmodulin binding peptide (CBP), a tobacco etch virus protease (TEV protease) cleavage site and Protein A) to the protein of interest. The TAP technique is useful in analyzing in vivo interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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