Gibco Neurobiology Protocol Handbook

Get a digital copy of 24 neurobiology protocols surrounding neural cell culture and differentiation, cell analysis, molecular characterization, and transfection. There are 6 new protocols in this updated version.

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Browse our Neurobiology protocols. You’ll find the protocols below along with the recommended components to build the optimal neural cell culture system for your research experiment.

Click on the protocol name to view the detailed protocol and full list of required materials.

Click on the product name in the right-hand columns to order.

Neural cell culture and dfferentiation protocols

Protocol Name Media Supplement Cells
Isolation, Culture, and Characterization of Cortical and Hippocampal Neurons  
Culturing Human Neural Stem Cells
Xeno-free Culture of Neural Stem Cells  
Differentiating Neural Stem Cells into Neurons and Glial Cells  
Differentiating Glial Precursor Cells into Astrocytes and Oligodendrocytes
Derivation of Dopaminergic Neurons (from Human Embryonic Stem Cells)
Culturing Rat Fetal Neural Stem Cells  
Derivation and Culture of Dopaminergic Neurons (from Midbrains of Rodents)  
Derivation and Culture of Cortical Astrocytes      
Cryopreserving Neural Stem Cells    
Cryopreservation and Recovery of Mature Differentiated Neural Cells  

We understand that there’s more to your experiment than culturing your cells.  View the protocols below for experiments beyond neural cell culture.

Click on the protocol name to view the detailed protocol and full list of required materials.

Cell analysis

Protocol Name
Cell Viability Assays for Neural Stem Cells
Markers for Characterizing Neural Subtypes
Surface Marker Analysis by Flow Cytometry
Intracellular Calcium Assay using Fluo-4

Molecular characterization

Protocol Name
PCR Primers for Molecular Characterization of Neural Subtypes
RNA Isolation and cDNA Preparation from Neural Stem Cells
Characterizing Neural Cells by qPCR


Protocol Name
Transfecting Neural Cells Using the Neon® Transfection System
Lipid-Mediated Transfection of Human Astrocytes