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Browse our Neurobiology protocols. You’ll find the protocols below along with the recommended components to build the optimal neural cell culture system for your research experiment.

Click on the protocol name to view the detailed protocol and full list of required materials.

Click on the product name in the right-hand columns to order.

Neural Cell Culture and Differentiation Protocols

Protocol Name Media Supplement Cells
Isolation, Culture, and Characterization of Cortical and Hippocampal Neurons

Neurobasal® Medium

Hibernate®-E Medium 

B-27® Serum Free Supplement   
Culturing Human Neural Stem CellsStemPro® NSC SFM media kit (kit includes media, growth factors, and supplement)GlutaMax™-I SupplementHuman neural stem cells
Xeno-free Culture of Neural Stem CellsNeurobasal® Medium

B-27® Supplement, XenoFree

GlutaMax™-I Supplement   

 
Differentiating Neural Stem Cells into Neurons and Glial Cells  

StemPro® NSC SFM media kit (kit includes media, growth factors, and supplement)

Neurobasal® Medium

DMEM

B-27® Serum Free Supplement

N-2 Supplement

GlutaMax™-I Supplement

Human neural stem cells
Differentiating Glial Precursor Cells into Astrocytes and Oligodendrocytes

Neurobasal® Medium

Astrocyte Medium (kit includes medium and supplements)

B-27® Serum Free SupplementRat glial precursor cells
Derivation of Dopaminergic Neurons (from Human Embryonic Stem Cells)

DMEM

DMEM/F-12 with GlutaMax™ -I

Neurobasal® Medium

KnockOut™ Serum Replacement

N-2 Supplement

B-27® Serum Free Supplement

Mouse embryonic fibroblasts
Culturing Rat Fetal Neural Stem CellsStemPro® NSC SFM media kit (kit includes media, growth factors, and supplement) Rat fetal neural stem cells
Derivation and Culture of Dopaminergic Neurons (from Midbrains of Rodents)Neurobasal® MediumB-27® Serum Free Supplement   
Derivation and Culture of Cortical Astrocytes  Astrocyte Medium (kit includes medium and supplements)  
Cryopreserving Neural Stem CellsStemPro® NSC SFM media kit   
 (kit includes media, growth factors, and supplement)
  
Cryopreservation and Recovery of Mature Differentiated Neural CellsNeurobasal® MediumB-27® Serum Free Supplement 

We understand that there’s more to your experiment than culturing your cells.  View the protocols below for experiments beyond neural cell culture.

Click on the protocol name to view the detailed protocol and full list of required materials.

Cell Analysis

Protocol Name
Cell Viability Assays for Neural Stem Cells
Markers for Characterizing Neural Subtypes
Surface Marker Analysis by Flow Cytometry
Immunocytochemistry
Intracellular Calcium Assay using Fluo-4

Molecular Characterization

Protocol Name
PCR Primers for Molecular Characterization of Neural Subtypes 
RNA Isolation and cDNA Preparation from Neural Stem Cells
Characterizing Neural Cells by qPCR

Transfection

Protocol Name
Transfecting Neural Cells Using the Neon® Transfection System
Lipid-Mediated Transfection of Human Astrocytes

For research use only. Not fur human or animal therapeutic or diagnostic use.