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Gibco Neurobiology Protocol Handbook

Download a digital copy of more than 20 neurobiology validated protocols for neural stem cell culture and differentiation, cell analysis, molecular characterization, and transfection. Review experimental best practices, and troubleshooting advice designed to get you started with your neurobiology research.

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Browse our Neurobiology protocols. You’ll find the protocols below along with the recommended components to build the optimal neural cell culture system for your research experiment.

Click on the protocol name to view the detailed protocol and full list of required materials.

Click on the product name in the right-hand columns to order.

Neural cell culture and differentiation protocols

Protocol nameHandbook chapterMediaSupplementCells
Recovery, culture, and characterization of primary neurons3,4
Creation of neural stem cells from human pluripotent stem cells9

Neural induction supplement

Human PSCs

Culturing human neural stem cells5

StemPro NSC SFM media kit (kit includes media, growth factors, and supplement)

GlutaMAX-I Supplement

Xeno-free culture of neural stem cells8

CTS Neurobasal Medium

Differentiating neural stem cells into neurons10

Neural stem cells derived using PSC Neural Induction Medium

Differentiating glial precursor cells into astrocytes and oligodendrocytes7

B-27 Plus Supplement

Rat glial precursor cells

Differentiating PSCs to midbrain dopaminergic neurons12 

Human PSCs

Culturing rat fetal neural stem cells6

StemPro NSC SFM media kit (kit includes media, growth factors, and supplement)

 

Rat fetal neural stem cells

Culture of cortical astrocytes13

Astrocyte Medium (kit includes medium and supplements)

B-27 Plus Supplement

Rat Primary Cortical Astrocytes

Cryopreserving neural stem cells14  

We understand that there’s more to your experiment than culturing your cells.  View the protocols below for experiments beyond neural cell culture.

Click on the protocol name to view the detailed protocol and full list of required materials.

Cell analysis

Molecular characterization

Transfection

For Research Use Only. Not for use in diagnostic procedures.