Protocols—pursue every angle

Gibco Neurobiology Protocol Handbook

Download a digital copy of more than 20 neurobiology validated protocols for neural stem cell culture and differentiation, cell analysis, molecular characterization, and transfection. Review experimental best practices, and troubleshooting advice designed to get you started with your neurobiology research.

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Browse our Neurobiology protocols. You’ll find the protocols below along with the recommended components to build the optimal neural cell culture system for your research experiment.

Click on the protocol name to view the detailed protocol and full list of required materials.

Click on the product name in the right-hand columns to order.

Neural cell culture and differentiation protocols

Protocol NameMediaSupplementCells
Isolation, Culture, and Characterization of Cortical and Hippocampal Neurons 
Culturing Human Neural Stem Cells
Xeno-free Culture of Neural Stem Cells 
Differentiating Neural Stem Cells into Neurons and Glial Cells  
Differentiating Glial Precursor Cells into Astrocytes and Oligodendrocytes
Derivation of Dopaminergic Neurons (from Human Embryonic Stem Cells)
Culturing Rat Fetal Neural Stem Cells 
Derivation and Culture of Dopaminergic Neurons (from Midbrains of Rodents) 
Derivation and Culture of Cortical Astrocytes    
Cryopreserving Neural Stem Cells  
Cryopreservation and Recovery of Mature Differentiated Neural Cells 

We understand that there’s more to your experiment than culturing your cells.  View the protocols below for experiments beyond neural cell culture.

Click on the protocol name to view the detailed protocol and full list of required materials.

Cell analysis

Molecular characterization

Transfection