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Invitrogen
KCNQ1 Monoclonal Antibody (N37A/10), APC
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FIGURE: 1 / 4
KCNQ1 Antibody (MA5-45407) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% PFA for 15 min. Samples were incubated with KCNQ1 monoclonal antibody (Product # MA5-45407) at 1:100 for overnight at 4°C with slow rocking, followed by AlexaFluor 488 at 1:1,000 for 1 hour at RT. Counterstain used was Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) KCNQ1 antibody (D) Composite.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-45407
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Hamster,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N37A/10
Immunogen
Fusion protein amino acids 2-101 of human KCNQ1
Conjugate
APC
APC
APC
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.46mM phosphate/2.48mM MES, pH 7.4, with 2mM EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-45407 was sufficient for detection of KCNQ1 in 10 µg of COS-1 cell lysate transiently expressing KCNQ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 75kDa.
Target Information
Voltage-gated K+ channels in the plasma membrane control the repolarization and the frequency of action potentials in neurons, muscles and other excitable cells. A specific K+ channel, comprised of an alpha subunit KCNQ1 and a beta subunit KCNE1, a small protein which spans the membrane only once, is predominantly expressed in the heart and in the cochlea, and is responsible for regulating the slow, depolarization-activated potassium current. Mutations in the genes encoding for KCNQ1 and KCNE1 lead to cardiac disease because they directly impair electrical signaling, and mutations in KCNQ4 are implicated in the onset of deafness. KCNQ proteins, including KCNQ1 and KCNQ4, characteristically contain six transmembrane domains and function as tetramers. KCNQ4 forms heteromeric channels with KCNQ3 and is expressed in several tissues, including the cochlea, where it is present in outer hair cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ26167; IKs producing slow voltage-gated potassium channel subunit alpha KvLQT1; KQT-like 1; Potassium voltage-gated channel subfamily KQT member 1; potassium voltage-gated channel, KQT-like subfamily, member 1; potassium voltage-gated channel, subfamily Q, member 1; Voltage-gated potassium channel subunit Kv7.1
Gene Aliases: AW559127; I79_015904; KCNA8; KCNA9; KCNQ1; KVLQT1
UniProt ID: (Mouse) P97414, (Rat) Q9Z0N7
Entrez Gene ID: (Mouse) 16535, (Chinese hamster) 100761481, (Rat) 84020
ion channel activity
voltage-gated ion channel activity
voltage-gated potassium channel activity
delayed rectifier potassium channel activity
potassium channel activity
calmodulin binding
protein phosphatase 1 binding
outward rectifier potassium channel activity
protein kinase A catalytic subunit binding
protein kinase A regulatory subunit binding
protein homodimerization activity
ion channel binding
voltage-gated potassium channel activity involved in cardiac muscle cell action potential repolarization
voltage-gated potassium channel activity involved in atrial cardiac muscle cell action potential repolarization
scaffold protein binding
voltage-gated potassium channel activity involved in ventricular cardiac muscle cell action potential repolarization
protein binding
voltage-gated ion channel
positive regulation of defense response to virus by host
regulation of gene expression by genetic imprinting
transport
ion transport
potassium ion transport
positive regulation of heart rate
gene silencing
regulation of ion transmembrane transport
cellular response to drug
regulation of membrane potential
negative regulation of insulin secretion
transmembrane transport
cardiac muscle contraction
regulation of membrane repolarization
regulation of ventricular cardiac muscle cell membrane repolarization
regulation of atrial cardiac muscle cell membrane repolarization
positive regulation of cardiac muscle contraction
positive regulation of gastric acid secretion
cellular response to cAMP
potassium ion export
potassium ion transmembrane transport
response to anesthetic
cardiovascular system development
ventricular cardiac muscle cell action potential
membrane repolarization during action potential
membrane repolarization during cardiac muscle cell action potential
atrial cardiac muscle cell action potential
regulation of heart rate by cardiac conduction
potassium ion export across plasma membrane
positive regulation of potassium ion transmembrane transport
male gonad development
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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