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Invitrogen

Gata-3 Monoclonal Antibody (TWAJ), PE, eBioscience™

8 Published Figures
82 References
View all (109) GATA3 antibodies

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Product Details

12-9966-41

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test
View 82 publications 82 publications
Product Specifications

Species Reactivity

Human, Mouse, Pig, Rhesus monkey

Published species

Human, Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

TWAJ

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1963601

Product Specific Information

Description: The monoclonal antibody TWAJ recognizes mouse and human Gata-3, a member of the Gata family of transcription factors. Gata-3 is a T cell-specific transcription factor important for thymic development and Th2 differentiation. Expression during embryonic development is found in the central nervous system, skin, mammary glands and kidney. During development, the expression of Gata-3 is essential as homozygous knock-out of Gata-3 is embryonic lethal. The Gata-3 is also essential for T cell commitment and survival. In the thymus, expression is found mainly on the CD4 single positive cells. During Th2 differentiation, Gata-3 binds to the IL-4 promoter as well as represses the expression of T-bet, thus inhibiting Th1 differentiation.

Alternative splice variants have been reported especially in the MCF7 cell line. The TWAJ Human/Mouse Gata-3 antibody will recognize both forms (50 and 45 kDa) of the protein.

Staining with the TWAJ Human/Mouse Gata-3 antibody requires the use of the Foxp3/Transcription Factor Staining Buffer Set.(Product # 00-5523-00)

Cross reactivity in rhesus monkeys has been published.

Applications Reported: This TWAJ antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This TWAJ antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523) and protocol. Please see BestProtocols® Section (Staining Intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

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Bioinformatics

Protein Aliases: GATA binding factor-3; GATA binding protein-3; GATA-3; GATA-binding factor 3; MGC2346; MGC5199; MGC5445; Transacting T-cell-specific transcription factor GATA-3; unnamed protein product

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Gene Aliases: EGK_19445; Gata-3; HDR; HDRS; jal

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UniProt ID: (Human) P23771, (Mouse) P23772

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Entrez Gene ID: (Pig) 733631, (Rhesus monkey) 713840, (Human) 2625, (Mouse) 14462

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding transcription coactivator binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding interleukin-2 receptor binding protein binding zinc ion binding identical protein binding sequence-specific DNA binding RNA polymerase II sequence-specific DNA binding transcription factor binding E-box binding HMG box domain binding histone methyltransferase binding sequence-specific double-stranded DNA binding DNA-binding transcription factor
Process(es)
negative regulation of transcription from RNA polymerase II promoter cell fate determination type IV hypersensitivity regulation of cytokine production kidney development mesonephros development immune system development aortic valve morphogenesis cardiac right ventricle morphogenesis ventricular septum development regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter defense response inflammatory response signal transduction negative regulation of cell proliferation male gonad development response to xenobiotic stimulus response to virus anatomical structure morphogenesis positive regulation of signal transduction response to gamma radiation gene expression positive regulation of endothelial cell migration negative regulation of epithelial to mesenchymal transition cell differentiation T cell differentiation macrophage differentiation regulation of epithelial cell differentiation TOR signaling positive regulation of interleukin-13 production positive regulation of interleukin-4 production positive regulation of interleukin-5 production negative regulation of mammary gland epithelial cell proliferation cellular response to interferon-alpha norepinephrine biosynthetic process protein kinase B signaling ear development response to estrogen T-helper 2 cell differentiation cell fate commitment response to ethanol positive regulation of T cell differentiation negative regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter sympathetic nervous system development embryonic organ development anatomical structure formation involved in morphogenesis system development negative regulation of inflammatory response regulation of developmental process T cell receptor signaling pathway cartilage development positive regulation of protein kinase B signaling pharyngeal system development uterus development canonical Wnt signaling pathway mesenchymal to epithelial transition ureteric bud formation cellular response to interleukin-4 cellular response to tumor necrosis factor positive regulation of ureteric bud formation nephric duct morphogenesis nephric duct formation regulation of nephron tubule epithelial cell differentiation lymphocyte migration cochlea development positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of cell motility negative regulation of endothelial cell apoptotic process positive regulation of thyroid hormone generation positive regulation of transcription regulatory region DNA binding regulation of cellular response to X-ray cell morphogenesis in utero embryonic development neuron migration cell activation positive regulation of cytokine production lens development in camera-type eye pro-T cell differentiation chromatin remodeling humoral immune response nervous system development axon guidance post-embryonic development positive regulation of gene expression negative regulation of gene expression regulation of neuron projection development neuron differentiation erythrocyte differentiation negative regulation of interferon-gamma production negative regulation of interleukin-2 production T cell differentiation in thymus embryonic hemopoiesis ureter maturation parathyroid hormone secretion inner ear morphogenesis regulation of CD4-positive, alpha-beta T cell differentiation regulation of neuron apoptotic process thymic T cell selection positive regulation of cell differentiation cell maturation animal organ development thymus development developmental growth homeostasis of number of cells regulation of multicellular organismal process parathyroid gland development mast cell differentiation cellular response to cytokine stimulus otic vesicle development cellular response to BMP stimulus ureter morphogenesis regulation of establishment of cell polarity positive regulation of T-helper 2 cell cytokine production
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