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          • Primary Antibodies ›
          • GATA3 Antibodies

          Invitrogen

          Gata-3 Monoclonal Antibody (TWAJ), Brilliant Ultra Violet™ 737, eBioscience™

          View all (105) GATA3 antibodies

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          Cite Gata-3 Monoclonal Antibody (TWAJ), Brilliant Ultra Violet™ 737, eBioscience™

          Additional Information:

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          • Antibody Testing Data (1)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          Gata-3 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          Gata-3 Antibody (367-9966-42) in Flow

          C57BL/6 mouse thymocytes were stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol, with CD4 Monoclonal Antibody, FITC (Product # 11-0041-82), CD8a Monoclonal Antibody, APC (Product # 17-0081-82), and Rat IgG2b kappa Isotype Control, Brilliant Ultra Violet 737 (Product # 367-4031-81) (blue histogram) or Gata-3 Monoclonal Antibody, Brilliant Ultra Violet 737 (purple histogram). Viable CD4 single-positive (left) or CD8 single-positive (right) cells were used for analysis, as de... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          Gata-3 Antibody in Flow Cytometry (Flow)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Product Details

          367-9966-42

          Applications
          Tested Dilution
          Publications

          Flow Cytometry (Flow)

          5 µL (0.5 µg)/test
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Pig, Rhesus monkey

          Host/Isotype

          Rat / IgG2b, kappa

          Recommended Isotype Control

          Rat IgG2b kappa Isotype Control (eB149/10H5), Brilliant Ultra Violet™ 737, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          TWAJ

          Conjugate

          Brilliant Ultra Violet™ 737 Brilliant Ultra Violet™ 737 Brilliant Ultra Violet™ 737
          • Unconjugated
          • Alexa Fluor 488 
          • Alexa Fluor 700 
          • Brilliant UV 395
          • Brilliant UV 563
          • Brilliant UV 661
          • Brilliant Violet 421
          • Brilliant Violet 480
          • Brilliant Violet 605
          • Brilliant Violet 711
          • Brilliant Violet 786
          • eFluor 450
          • eFluor 660
          • PE 
          • PE-Cyanine5
          • PE-Cyanine7
          • PE-eFluor 610
          • PerCP-eFluor 710
          • Request custom conjugation

          Excitation/Emission Max

          350/740 nm View spectra spectra

          Form

          liquid

          Concentration

          5 µL/Test

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2, with BSA

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_3699339

          Product Specific Information

          Description
          The monoclonal antibody TWAJ recognizes mouse and human Gata-3, a member of the Gata family of transcription factors. Gata-3 is a T cell-specific transcription factor important for thymic development and Th2 differentiation. Expression during embryonic development is found in the central nervous system, skin, mammary glands and kidney. During development, the expression of Gata-3 is essential as homozygous knock-out of Gata-3 is embryonic lethal. The Gata-3 is also essential for T cell commitment and survival. In the thymus, expression is found mainly on the CD4 single positive cells. During Th2 differentiation, Gata-3 binds to the IL-4 promoter as well as represses the expression of T-bet, thus inhibiting Th1 differentiation. Alternative splice variants have been reported especially in the MCF7 cell line. The TWAJ Human/Mouse Gata-3 antibody will recognize both forms (50 and 45 kDa) of the protein. Staining with the TWAJ Human/Mouse Gata-3 antibody requires the use of the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00). Crossreactivity in rhesus monkeys has been published.

          Applications Tested
          This TWAJ antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins" located at Flow Protocols. This may be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

          Blocking Buffers
          When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.

          Light sensitivity
          This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

          Fixation
          • Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.
          • Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
          • Our internal testing suggests that Brilliant Ultra Violet™ 737 (BUV737) is compatible with short-term methanol-based fixation, but should not be stored in buffers containing methanol for longer than one hour.

          Excitation: 355 nm; Emission: 732 nm; Laser: Ultraviolet Laser.

          BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

          Target Information

          The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

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          Bioinformatics

          Protein Aliases: GATA binding factor-3; GATA binding protein-3; GATA-3; GATA-binding factor 3; MGC2346; MGC5199; MGC5445; Transacting T-cell-specific transcription factor GATA-3; unnamed protein product

          View more View less

          Gene Aliases: EGK_19445; Gata-3; HDR; HDRS; jal

          View more View less

          UniProt ID: (Human) P23771, (Mouse) P23772

          View more View less

          Entrez Gene ID: (Pig) 733631, (Rhesus monkey) 713840, (Human) 2625, (Mouse) 14462

          View more View less

          Function(s)
          transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding transcription coactivator binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding interleukin-2 receptor binding protein binding zinc ion binding identical protein binding sequence-specific DNA binding RNA polymerase II sequence-specific DNA binding transcription factor binding E-box binding HMG box domain binding histone methyltransferase binding sequence-specific double-stranded DNA binding DNA-binding transcription factor
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter cell fate determination type IV hypersensitivity regulation of cytokine production kidney development mesonephros development immune system development aortic valve morphogenesis cardiac right ventricle morphogenesis ventricular septum development regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter defense response inflammatory response signal transduction negative regulation of cell proliferation male gonad development response to xenobiotic stimulus response to virus anatomical structure morphogenesis positive regulation of signal transduction response to gamma radiation gene expression positive regulation of endothelial cell migration negative regulation of epithelial to mesenchymal transition cell differentiation T cell differentiation macrophage differentiation regulation of epithelial cell differentiation TOR signaling positive regulation of interleukin-13 production positive regulation of interleukin-4 production positive regulation of interleukin-5 production negative regulation of mammary gland epithelial cell proliferation cellular response to interferon-alpha norepinephrine biosynthetic process protein kinase B signaling ear development response to estrogen T-helper 2 cell differentiation cell fate commitment response to ethanol positive regulation of T cell differentiation negative regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter sympathetic nervous system development embryonic organ development anatomical structure formation involved in morphogenesis system development negative regulation of inflammatory response regulation of developmental process T cell receptor signaling pathway cartilage development positive regulation of protein kinase B signaling pharyngeal system development uterus development canonical Wnt signaling pathway mesenchymal to epithelial transition ureteric bud formation cellular response to interleukin-4 cellular response to tumor necrosis factor positive regulation of ureteric bud formation nephric duct morphogenesis nephric duct formation regulation of nephron tubule epithelial cell differentiation lymphocyte migration cochlea development positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of cell motility negative regulation of endothelial cell apoptotic process positive regulation of thyroid hormone generation positive regulation of transcription regulatory region DNA binding regulation of cellular response to X-ray cell morphogenesis in utero embryonic development neuron migration cell activation positive regulation of cytokine production lens development in camera-type eye pro-T cell differentiation chromatin remodeling humoral immune response nervous system development axon guidance post-embryonic development positive regulation of gene expression negative regulation of gene expression regulation of neuron projection development neuron differentiation erythrocyte differentiation negative regulation of interferon-gamma production negative regulation of interleukin-2 production T cell differentiation in thymus embryonic hemopoiesis ureter maturation parathyroid hormone secretion inner ear morphogenesis regulation of CD4-positive, alpha-beta T cell differentiation regulation of neuron apoptotic process thymic T cell selection positive regulation of cell differentiation cell maturation animal organ development thymus development developmental growth homeostasis of number of cells regulation of multicellular organismal process parathyroid gland development mast cell differentiation cellular response to cytokine stimulus otic vesicle development cellular response to BMP stimulus ureter morphogenesis regulation of establishment of cell polarity positive regulation of T-helper 2 cell cytokine production
          It has to be done as per old AB suggested Products section.

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