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          • Primary Antibodies ›
          • GATA3 Antibodies

          Invitrogen

          Gata-3 Monoclonal Antibody (TWAJ), PerCP-eFluor™ 710, eBioscience™

          19 Published Figures
          35 References
          View all (84) GATA3 antibodies

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          Cite Gata-3 Monoclonal Antibody (TWAJ), PerCP-eFluor™ 710, eBioscience™

          Additional Information:
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          • Antibody Testing Data (1)
          • Published Figures (19)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          Gata-3 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 20

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          Gata-3 Antibody (46-9966-42) in Flow

          BALB/c thymocytes were surface stained with Anti-Mouse CD4 APC (Product # 17-0041-82) and Anti-Mouse CD8a PE (Product # 12-0081-82) followed by intracellular staining with Rat IgG2b K Isotype Control PerCP-eFluor® 710 (Product # 46-4031-82) (blue histogram) or Anti-Human/Mouse Gata-3 PerCP-eFluor® 710 (purple histogram) using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Cells in the CD4 single positive (left) or CD8 single positive (right) gates were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)

          Product Details

          46-9966-42

          Applications
          Tested Dilution
          Publications

          Flow Cytometry (Flow)

          5 µL (0.06 µg)/test
          View 35 publications 35 publications
          Product Specifications

          Species Reactivity

          Human, Mouse, Pig, Rhesus monkey

          Published species

          Human, Mouse, Pig

          Host/Isotype

          Rat / IgG2b, kappa

          Recommended Isotype Control

          Rat IgG2b kappa Isotype Control (eB149/10H5), PerCP-eFluor™ 710, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          TWAJ

          Conjugate

          PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710
          • Unconjugated
          • Alexa Fluor 488 
          • Alexa Fluor 700 
          • Brilliant UV 395
          • Brilliant UV 563
          • Brilliant UV 661
          • Brilliant UV 737
          • Brilliant Violet 421
          • Brilliant Violet 480
          • Brilliant Violet 605
          • Brilliant Violet 711
          • Brilliant Violet 786
          • eFluor 450
          • eFluor 660
          • PE 
          • PE-Cyanine5
          • PE-Cyanine7
          • PE-eFluor 610
          • Request custom conjugation

          Excitation/Emission Max

          482/708 nm View spectra spectra

          Form

          Liquid

          Concentration

          5 µL/Test

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2, with BSA

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10804487

          Product Specific Information

          Description: The monoclonal antibody TWAJ recognizes mouse and human Gata-3, a member of the Gata family of transcription factors. Gata-3 is a T cell-specific transcription factor important for thymic development and Th2 differentiation. Expression during embryonic development is found in the central nervous system, skin, mammary glands and kidney. During development, the expression of Gata-3 is essential as homozygous knock-out of Gata-3 is embryonic lethal. The Gata-3 is also essential for T cell commitment and survival. In the thymus, expression is found mainly on the CD4 single positive cells. During Th2 differentiation, Gata-3 binds to the IL-4 promoter as well as represses the expression of T-bet, thus inhibiting Th1 differentiation.

          Alternative splice variants have been reported especially in the MCF7 cell line. The TWAJ Human/Mouse Gata-3 antibody will recognize both forms (50 and 45 kDa) of the protein.

          Staining with the TWAJ Human/Mouse Gata-3 antibody requires the use of the Foxp3/Transcription Factor Staining Buffer Set.(Product # 00-5523-00)

          Cross reactivity in rhesus monkeys has been published.

          Applications Reported: This TWAJ antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

          Applications Tested: This TWAJ antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523) and protocol. Please refer to BestProtocols®: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

          PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

          Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

          Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

          Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: GATA binding factor-3; GATA binding protein-3; GATA-3; GATA-binding factor 3; GATA-binding protein 3; MGC2346; MGC5199; MGC5445; Trans-acting T-cell-specific transcription factor GATA-3; Transacting T-cell-specific transcription factor GATA-3; unnamed protein product

          View more View less

          Gene Aliases: EGK_19445; Gata-3; GATA3; HDR; HDRS; jal

          View more View less

          UniProt ID: (Human) P23771, (Mouse) P23772

          View more View less

          Entrez Gene ID: (Pig) 733631, (Rhesus monkey) 713840, (Human) 2625, (Mouse) 14462

          View more View less

          Function(s)
          transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding transcription coactivator binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding interleukin-2 receptor binding protein binding zinc ion binding identical protein binding sequence-specific DNA binding metal ion binding RNA polymerase II sequence-specific DNA binding transcription factor binding E-box binding HMG box domain binding histone methyltransferase binding sequence-specific double-stranded DNA binding RNA polymerase II core promoter sequence-specific DNA binding core promoter sequence-specific DNA binding nucleic acid binding transcription factor activity transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding enhancer sequence-specific DNA binding transcription coactivator activity transcription factor binding transcription regulatory region DNA binding protein dimerization activity DNA-binding transcription factor
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter cell morphogenesis in utero embryonic development cell fate determination neuron migration cell activation type IV hypersensitivity regulation of cytokine production positive regulation of cytokine production kidney development mesonephros development lens development in camera-type eye immune system process immune system development pro-T cell differentiation aortic valve morphogenesis cardiac right ventricle morphogenesis ventricular septum development chromatin remodeling regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter defense response inflammatory response humoral immune response signal transduction nervous system development axon guidance cell proliferation negative regulation of cell proliferation male gonad development response to xenobiotic stimulus response to virus anatomical structure morphogenesis post-embryonic development positive regulation of signal transduction response to gamma radiation gene expression positive regulation of endothelial cell migration positive regulation of gene expression negative regulation of gene expression negative regulation of epithelial to mesenchymal transition regulation of neuron projection development cell differentiation neuron differentiation T cell differentiation erythrocyte differentiation macrophage differentiation regulation of epithelial cell differentiation TOR signaling negative regulation of interferon-gamma production negative regulation of interleukin-2 production positive regulation of interleukin-13 production positive regulation of interleukin-4 production positive regulation of interleukin-5 production T cell differentiation in thymus negative regulation of mammary gland epithelial cell proliferation embryonic hemopoiesis cellular response to interferon-alpha ureter maturation parathyroid hormone secretion norepinephrine biosynthetic process inner ear morphogenesis protein kinase B signaling regulation of neuron apoptotic process ear development response to estrogen thymic T cell selection T-helper 2 cell differentiation innate immune response cell fate commitment response to ethanol positive regulation of T cell differentiation positive regulation of cell differentiation regulation of T-helper cell differentiation negative regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cell maturation sympathetic nervous system development thymus development embryonic organ development developmental growth anatomical structure formation involved in morphogenesis system development homeostasis of number of cells negative regulation of inflammatory response regulation of developmental process T cell receptor signaling pathway cartilage development positive regulation of protein kinase B signaling parathyroid gland development pharyngeal system development uterus development canonical Wnt signaling pathway mesenchymal to epithelial transition mast cell differentiation ureteric bud formation cellular response to cytokine stimulus cellular response to interleukin-4 cellular response to tumor necrosis factor otic vesicle development cellular response to BMP stimulus positive regulation of ureteric bud formation nephric duct morphogenesis nephric duct formation regulation of nephron tubule epithelial cell differentiation ureter morphogenesis lymphocyte migration cochlea development positive regulation of pri-miRNA transcription from RNA polymerase II promoter regulation of establishment of cell polarity negative regulation of cell motility negative regulation of endothelial cell apoptotic process positive regulation of T-helper 2 cell cytokine production negative regulation of cell proliferation involved in mesonephros development positive regulation of thyroid hormone generation positive regulation of transcription regulatory region DNA binding regulation of cellular response to X-ray negative regulation of fibroblast growth factor receptor signaling pathway involved in ureteric bud formation negative regulation of glial cell-derived neurotrophic factor receptor signaling pathway involved in ureteric bud formation transcription, DNA-templated phosphatidylinositol 3-kinase signaling regulation of cytokine biosynthetic process regulation of CD4-positive, alpha-beta T cell differentiation negative regulation of fat cell differentiation regulation of histone H3-K4 methylation regulation of histone H3-K27 methylation canonical Wnt signaling pathway involved in metanephric kidney development renal system development interleukin-4 secretion interferon-gamma secretion positive regulation of interleukin-5 secretion positive regulation of interleukin-13 secretion
          It has to be done as per old AB suggested Products section.

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