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Invitrogen
IL-4 Monoclonal Antibody (11B11), Functional Grade, eBioscience™
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
16-7041-38
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
11B11
Conjugate
Functional Grade
Functional Grade
Functional Grade
Form
Liquid
Concentration
1 mg/mL
Amount
5 mg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The 11B11 antibody reacts with mouse interleukin-4 (IL-4), a 14 kD cytokine. The 11B11 antibody is a neutralizing antibody. mouse IL-4, also known as BCDF, BCGF-1 and BSF-1, is secreted by activated Th2 and natural killer T cells, and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4, in conjunction with other signals such as CD40, induces Ig isotype switching of B cells and the upregulation of surface MHC class II and CD23.
Applications Reported: This 11B11 antibody has been reported for use in cytokine neutralization, ELISPOT, and ELISA cap.
Applications Tested: The Functional Grade Purified 11B11 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of mouse IL-4 bioactivity.
The 11B11 antibody at 0.125 µg/mL has been found to inhibit by 50% the biological effects of 1.0 ng/mL mouse IL-4 in a CTLL-2 cell proliferation assay. Please refer to BestProtocols® for more information.
The 11B11 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse interleukin-4 (IL-4) in combination with the biotinylated BVD6-24G2 (Product # 13-7042-85) antibody for detection and recombinant mouse IL-4 (Product # 14-8041-80) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/mL - 4 pg/mL should be included in each ELISA plate.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; H-IL-4; IGG1 induction factor; IL4; ILN; Interleukin; interleukin mRNA; Interleukin4; lymphocyte stimulatory factor 1; M-IL-4; MGC79402; unnamed protein product
Gene Aliases: BSF-1; Il-4
UniProt ID: (Mouse) P07750
Entrez Gene ID: (Mouse) 16189
negative regulation of transcription from RNA polymerase II promoter
microglial cell activation
positive regulation of protein phosphorylation
innate immune response in mucosa
positive regulation of defense response to virus by host
T-helper 1 cell lineage commitment
positive regulation of immunoglobulin production
negative regulation of acute inflammatory response
negative regulation of chronic inflammatory response
positive regulation of leukocyte chemotaxis
negative regulation of leukocyte activation
immune response
JAK-STAT cascade
cholesterol metabolic process
positive regulation of cell proliferation
response to nematode
regulation of proton transport
response to selenium ion
positive regulation of gene expression
negative regulation of epithelial cell migration
positive regulation of macroautophagy
positive regulation of cell migration
positive regulation of B cell proliferation
B cell costimulation
negative regulation of tumor necrosis factor production
positive regulation of chemokine production
positive regulation of interleukin-10 production
positive regulation of interleukin-13 production
interleukin-4-mediated signaling pathway
positive regulation of T cell proliferation
positive regulation of activated T cell proliferation
T cell activation
macrophage activation
positive regulation of tyrosine phosphorylation of STAT protein
defense response to protozoan
myeloid dendritic cell differentiation
negative regulation of macrophage activation
positive regulation of mast cell degranulation
negative regulation of nitric oxide biosynthetic process
T-helper 2 cell differentiation
leukocyte activation
positive regulation of MHC class II biosynthetic process
positive regulation of T cell differentiation
negative regulation of osteoclast differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of receptor-mediated endocytosis
positive regulation of isotype switching to IgE isotypes
positive regulation of isotype switching to IgG isotypes
negative regulation of inflammatory response
positive regulation of peptidyl-tyrosine phosphorylation
regulation of immune response
negative regulation of T cell activation
positive regulation of B cell activation
negative regulation of white fat cell proliferation
positive regulation of mononuclear cell migration
dendritic cell differentiation
extrinsic apoptotic signaling pathway in absence of ligand
positive regulation of cold-induced thermogenesis
neuroinflammatory response
positive regulation of beta-amyloid clearance
positive regulation of myoblast fusion
positive regulation of cellular respiration
positive regulation of reactive oxygen species biosynthetic process
negative regulation of complement-dependent cytotoxicity
negative regulation of cellular response to transforming growth factor beta stimulus
negative regulation of T-helper 17 cell differentiation
negative regulation of endothelial cell apoptotic process
positive regulation of eosinophil chemotaxis
positive regulation of B cell chemotaxis
positive regulation of T-helper 2 cell cytokine production
positive regulation of ATP biosynthetic process
negative regulation of extrinsic apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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