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Nur77 Monoclonal Antibody (12.14), PerCP-eFluor™ 710, eBioscience™
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FIGURE: 1 / 5
Nur77 Antibody (46-5965-82) in Flow
Mouse thymocytes were unstimulated (left) or stimulated for 3 hours with the Cell Stimulation Cocktail (Product # 00-4970) (right) then intracellularly stained with Anti-Mouse CD4 APC (Product # 17-0041) and 0.06 µg of Anti-Mouse Nur77 PerCP-eFluor® 710 using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523) and protocol. Total viable cells, as determined by Fixable Viability Dye eFluor® 506 (Product # 65-0866), were used for analysis.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
46-5965-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
12.14
Conjugate
PerCP-eFluor™ 710
PerCP-eFluor™ 710
PerCP-eFluor™ 710
Excitation/Emission Max
482/708 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This 12.14 monoclonal antibody reacts with mouse Nur77 (also known as NR4A1, TR3, NGFI-B, or NAK1), an inducible orphan nuclear receptor. Expressed in thymocytes and T cell lines, Nur77 promotes apoptosis and plays a role in thymocyte negative selection. Additionally, Nur77 has been shown to be critical for steroid biosynthesis in Leydig cells as well as for the effects of dopamine. In addition, Nur77 has been shown to interact with FoxP3 in regulatory T cells. However, our results with this antibody do not correlate with this observation.
Applications Reported: This 12.14 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This 12.14 antibody has been tested by intracellular staining followed by flow cytometric analysis of stimulated mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to BestProtocols®: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
NR4A1 is a member of the steroid-thyroid hormone-retinoid receptor superfamily. NGFI-B-alpha is expressed in brain, adrenal, thyroid, liver, testis, ovary, thymus, muscle, lung and prostate. Its expression is induced in response to various stress stimuli and growth factors. The encoded protein acts as a nuclear transcription factor involved in mediating apoptotic signaling in thymocytes and tumor cells, as well as having a role in signaling in the hypothalamic-pituitary axis. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Three transcript variants encoding two distinct isoforms have been identified for this NR4A1. Variant (3), also known as TRC beta, contains multiple differences compared to variant 1. The resulting isoform b contains a shorter and distinct C-terminus compared to isoform a. RXR has been shown to be a partner for NR4A1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hormone receptor; MGC9485; NR4A; Nr4a1; nuclear hormone receptor NUR/77; nuclear protein N10; orphan nuclear receptor HMR; Orphan receptor tr3; unnamed protein product
Gene Aliases: Gfrp; GFRP1; Hbr-1; Hbr1; Hmr; N10; NGFI-B; NGFIB; NP10; nur77; NUR77-1; NUR77-2; TIS1; TR3
UniProt ID: (Mouse) P12813
Entrez Gene ID: (Mouse) 15370
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
lipopolysaccharide binding
DNA binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein binding
zinc ion binding
glucocorticoid receptor binding
identical protein binding
sequence-specific DNA binding
protein heterodimerization activity
sequence-specific double-stranded DNA binding
positive regulation of endothelial cell proliferation
response to amphetamine
cell migration involved in sprouting angiogenesis
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
protein import into nucleus
apoptotic process
neurotransmitter secretion involved in regulation of skeletal muscle contraction
intracellular receptor signaling pathway
detection of lipopolysaccharide
endothelial cell chemotaxis
skeletal muscle cell differentiation
cellular response to vascular endothelial growth factor stimulus
macrophage activation
regulation of apoptotic process
positive regulation of apoptotic process
cellular response to fibroblast growth factor stimulus
fat cell differentiation
negative regulation of cell cycle
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of type B pancreatic cell proliferation
cellular response to corticotropin-releasing hormone stimulus
non-canonical inflammasome complex assembly
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