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Invitrogen
Nur77 Monoclonal Antibody (12.14), eBioscience™
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Product Details
14-5965-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
12.14
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This 12.14 monoclonal antibody reacts with mouse Nur77 (also known as NR4A1, TR3, NGFI-B, or NAK1), an inducible orphan nuclear receptor. Expressed in thymocytes and T cell lines, Nur77 promotes apoptosis and plays a role in thymocyte negative selection. Additionally, Nur77 has been shown to be critical for steroid biosynthesis in Leydig cells as well as for the effects of dopamine. In addition, Nur77 has been shown to interact with FoxP3 in regulatory T cells. However, our results with this antibody do not correlate with this observation.
Applications Reported: This 12.14 antibody has been reported for use in immunoblotting (WB).
Applications Tested: This 12.14 antibody has been tested by western blot on lysates prepared from mouse thymocytes stimulated with PMA and ionomycin for two hours. This antibody can be used at 5 µg/mL. It is recommended that this antibody by titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
NR4A1 is a member of the steroid-thyroid hormone-retinoid receptor superfamily. NGFI-B-alpha is expressed in brain, adrenal, thyroid, liver, testis, ovary, thymus, muscle, lung and prostate. Its expression is induced in response to various stress stimuli and growth factors. The encoded protein acts as a nuclear transcription factor involved in mediating apoptotic signaling in thymocytes and tumor cells, as well as having a role in signaling in the hypothalamic-pituitary axis. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Three transcript variants encoding two distinct isoforms have been identified for this NR4A1. Variant (3), also known as TRC beta, contains multiple differences compared to variant 1. The resulting isoform b contains a shorter and distinct C-terminus compared to isoform a. RXR has been shown to be a partner for NR4A1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: hormone receptor; MGC9485; Nuclear hormone receptor NUR/77; Nuclear protein N10; Nuclear receptor subfamily 4 group A member 1; Orphan nuclear receptor HMR; Orphan receptor tr3
Gene Aliases: Gfrp; GFRP1; Hbr-1; Hbr1; Hmr; N10; NGFI-B; NGFIB; NP10; Nr4a1; Nur77; TIS1; TR3
UniProt ID: (Mouse) P12813
Entrez Gene ID: (Mouse) 15370
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
steroid hormone receptor activity
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein binding
zinc ion binding
protein homodimerization activity
sequence-specific DNA binding
metal ion binding
protein heterodimerization activity
C4 zinc finger nuclear receptor
positive regulation of endothelial cell proliferation
cell migration involved in sprouting angiogenesis
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
apoptotic process
response to inorganic substance
endothelial cell chemotaxis
skeletal muscle cell differentiation
cellular response to vascular endothelial growth factor stimulus
positive regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
steroid hormone mediated signaling pathway
cellular response to fibroblast growth factor stimulus
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cellular response to organic substance
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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