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Invitrogen

TNF alpha Monoclonal Antibody (MAb11), PE-eFluor™ 610, eBioscience™

26 Published Figures
23 References
View all (216) TNF alpha antibodies

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Product Details

61-7349-42

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test
View 23 publications 23 publications
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-eFluor™ 610, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MAb11

Conjugate

PE-eFluor™ 610 PE-eFluor™ 610 PE-eFluor™ 610

Excitation/Emission Max

565/606 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2574668

Product Specific Information

Description: The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TN alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26 kDa membrane bound form. The MAb11 antibody is a neutralizing antibody.

Applications Reported: This MAb11 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This MAb11 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to BestProtocols®: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: APC1 protein; cell apoptosis-related protein; DADB-70P7.1; TNF alpha; TNF superfamily; TNF superfamily member 2; TNF α; TNF, macrophage-derived; TNF, monocyte-derived; TNF-a; TNFα; tumor necrosis factor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha; tumor necrotic factor alpha; unnamed protein product

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Gene Aliases: DIF; IMD127; TNF-alpha; TNFA; TNFSF2; TNLG1F

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UniProt ID: (Human) P01375

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Entrez Gene ID: (Human) 7124

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Function(s)
transcription regulatory region sequence-specific DNA binding protease binding cytokine activity tumor necrosis factor receptor binding protein binding identical protein binding receptor agonist activity histone H3K9ac reader activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter response to hypoxia microglial cell activation positive regulation of cytokine production negative regulation of endothelial cell proliferation response to amphetamine negative regulation of L-glutamate transport macrophage activation involved in immune response chronic inflammatory response to antigenic stimulus positive regulation of immunoglobulin production regulation of production of molecular mediator of immune response negative regulation of cytokine production involved in immune response skeletal muscle contraction negative regulation of systemic arterial blood pressure chromatin organization inflammatory response immune response JAK-STAT cascade circadian rhythm negative regulation of cell proliferation extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to DNA damage response to xenobiotic stimulus response to mechanical stimulus response to virus response to bacterium response to salt stress response to fructose negative regulation of heart rate vascular endothelial growth factor production positive regulation of gene expression negative regulation of gene expression positive regulation of macrophage derived foam cell differentiation negative regulation of lipid storage response to activity calcium-mediated signaling positive regulation of protein complex assembly positive regulation of defense response positive regulation of fever generation negative regulation of myelination lipopolysaccharide-mediated signaling pathway response to nutrient levels response to lipopolysaccharide negative regulation of interleukin-6 production positive regulation of chemokine production positive regulation of fractalkine production positive regulation of interleukin-1 beta production positive regulation of interleukin-18 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production tumor necrosis factor-mediated signaling pathway positive regulation of heterotypic cell-cell adhesion toll-like receptor 3 signaling pathway response to isolation stress response to macrophage colony-stimulating factor regulation of cell proliferation vasodilation positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of programmed cell death regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of protein complex disassembly positive regulation of MAPK cascade positive regulation of neuron apoptotic process negative regulation of blood vessel endothelial cell migration negative regulation of viral genome replication innate immune response positive regulation of nitric oxide biosynthetic process response to ethanol regulation of fat cell differentiation negative regulation of fat cell differentiation negative regulation of osteoblast differentiation positive regulation of osteoclast differentiation positive regulation of protein catabolic process positive regulation of action potential positive regulation of cell adhesion positive regulation of mitotic nuclear division negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated negative regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter negative regulation of glucose import positive regulation of JNK cascade response to antibiotic astrocyte activation embryonic digestive tract development positive regulation of smooth muscle cell proliferation regulation of epithelial cell proliferation positive regulation of inflammatory response negative regulation of neurogenesis regulation of developmental process regulation of insulin secretion modulation of synaptic transmission positive regulation of synaptic transmission regulation of synapse organization cognition leukocyte tethering or rolling detection of mechanical stimulus involved in sensory perception of pain negative regulation of lipid catabolic process positive regulation of membrane protein ectodomain proteolysis regulation of secretion positive regulation of protein transport regulation of multicellular organismal process response to glucocorticoid positive regulation of hair follicle development positive regulation of protein kinase B signaling regulation of synaptic transmission, glutamatergic positive regulation of glial cell proliferation positive regulation of vitamin D biosynthetic process negative regulation of vascular wound healing negative regulation of branching involved in lung morphogenesis positive regulation of ERK1 and ERK2 cascade positive regulation of calcineurin-NFAT signaling cascade cellular response to molecule of bacterial origin cellular response to lipopolysaccharide cellular response to retinoic acid cellular response to nicotine cellular response to interferon-gamma cellular response to ionizing radiation positive regulation of mononuclear cell migration positive regulation of podosome assembly endothelial cell apoptotic process protein localization to plasma membrane negative regulation of oxidative phosphorylation inflammatory response to wounding extrinsic apoptotic signaling pathway cellular response to toxic substance liver regeneration necroptotic signaling pathway negative regulation of bile acid secretion antiviral innate immune response response to Gram-negative bacterium positive regulation of neuroinflammatory response positive regulation of interleukin-33 production positive regulation of cytokine production involved in inflammatory response negative regulation of beta-amyloid clearance positive regulation of NIK/NF-kappaB signaling positive regulation of synoviocyte proliferation positive regulation of beta-amyloid formation response to L-glutamate positive regulation of neutrophil activation negative regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of protein localization to plasma membrane regulation of establishment of endothelial barrier negative regulation of bicellular tight junction assembly positive regulation of cellular protein localization response to resveratrol cellular response to beta-amyloid positive regulation of vascular smooth muscle cell proliferation response to hydrogen sulfide positive regulation of leukocyte adhesion to vascular endothelial cell positive regulation of leukocyte adhesion to arterial endothelial cell response to quercetin response to 3,3',5-triiodo-L-thyronine response to gold nanoparticle positive regulation of protein localization to cell surface positive regulation of blood microparticle formation positive regulation of chemokine (C-X-C motif) ligand 2 production positive regulation of hepatocyte proliferation regulation of endothelial cell apoptotic process regulation of reactive oxygen species metabolic process positive regulation of DNA biosynthetic process negative regulation of apoptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
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