B-27™ Supplement (50X), minus vitamin A

Citations & References (2)

Gibco™

B-27™ Supplement (50X), minus vitamin A

Name: B-27™ Supplement (50X), minus vitamin A
SKU: 12587010

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製品番号（カタログ番号）	数量
12587010	10 mL
12587001	100 mL

2 オプション

製品番号（カタログ番号） 12587010

価格（JPY）

39,700

Each

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数量:

10 mL

最も多く文献で引用されている神経細胞培養補完のバリエーションであるB-27補完マイナスビタミンAは、ビタミンA（酢酸レチニル）を含まないオリジナルのB-27補完のカスタマイズバージョンです。B-27補完マイナスビタミンAは無血清の補完で、懸濁液中の神経球体として、または接着性単層培養において、分化を誘発しない神経前駆細胞および幹細胞の培養に最適です。B-27補完マイナスビタミンAは50倍の液体として提供され、星状膠細胞フィーダー層を必要とせずに、神経細胞培養用のNeurobasal培地またはNeurobasal-A培地で使用することを目的としています。

B-27補完は、ほとんどの神経細胞アプリケーションでの使用に適しています。その他のアプリケーションのために、製剤B-27補完の代替およびNeurobasa培地をご用意しています。

B-27補完を使用して確立されたプロトコルについては、Gibco神経生物学プロトコルハンドブックをご覧ください。

Gibco神経生物学製品を使用した出版物については、Gibco CellCiteをご覧ください。

研究用にのみ使用できます。診断用には使用いただけません。

仕様

細胞タイプNeural Progenitor Cells

濃度50 X

培養タイプMammalian Cell Culture

製品ラインB-27

数量10 mL

血清レベルSerum-Free

品質保持期間12 Months

出荷条件Dry Ice

電子線源Serum-Free

試験済みEndotoxin, Performance, Sterility, Mycoplasma

分類Serum-free

形状Liquid

製品タイプCell Culture Supplement

無菌性Sterile

Unit SizeEach

組成および保存条件

Store in freezer (-5 to -20°C) and protect from light.

よくあるご質問（FAQ）

Can B-27 supplement be filtered?

Yes, the protein content in this product is high enough so filtering through a low protein binding filter as a 50X or 1X in solution should not be a problem.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can Neurobasal Medium alone or Neurobasal Medium supplemented with B-27 supplement be frozen to extend product shelf life?

No, it is not recommended to freeze media due to the potential of precipitates forming upon thaw. Inorganic salts and amino acids in the formulation may come out of solution when exposed to temperature fluctuations. These precipitates will not go back into solution easily once formed.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What supplements should I use with Neurobasal or Neurobasal-A medium?

Neurobasal and Neurobasal-A media (for postnatal and adult neurons) allow for long-term maintenance of neuronal cells without the need for an astrocyte feeder layer. These media should be supplemented with either serum or a serum-free supplement, plus 0.5mM L-glutamine. B-27 supplement is a serum-free supplement that comes as a 50X concentrate in a 10ml volume. This is enough supplement for 500ml of media. Fetal, postnatal, and adult neural cultures can be grown in the appropriate Neurobasal medium supplemented with B-27 supplement .

We also have two other supplements. One is called G-5 and is for growth and expression of glial cells (normal and tumor) of astrocytic phenotype. This comes in a 1ml size, at a 100X concentration. The other supplement is called N-2 and is for growth and expression of post-mitotic neurons and tumor cells of neuronal phenotype. This comes in a 5ml size, at a 100X concentration.

For more information on Neurobasal media, search "Neurobasal" from our website home page.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer B-27 supplements with and without insulin?

Yes, we do offer B-27 Supplement, minus insulin (Cat. Nos. A1895601 and A1895602). All our other B-27 supplements do contain insulin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I thaw B-27 Supplements in a 37 degrees C water bath or incubator?

We do not recommend thawing at 37 degrees C. Best results are observed upon thawing at room temperature for approximately 2 hrs or overnight at 2-8 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all B-27™ Supplement (50X), minus vitamin A FAQs

引用および参考文献 (2)

引用および参考文献

Abstract

Patient-derived glioblastoma organoids as real-time avatars for assessing responses to clinical CAR-T cell therapy

Authors:Meghan Logun, Xin Wang, Yusha Sun, Stephen J Bagley, Nannan Li, Arati Desai, Daniel Y Zhang, MacLean P Nasrallah, Emily Ling-Lin Pai, Bike Su Oner, Gabriela Plesa, Donald Siegel, Zev A Binder, Guo-Li Ming, Hongjun Song, Donald M O'Rourke

Journal:Cell Stem Cell

PubMed ID:39657679

Patient-derived tumor organoids have been leveraged for disease modeling and preclinical studies but rarely applied in real time to aid with interpretation of patient treatment responses in clinics. We recently demonstrated early efficacy signals in a first-in-human, phase 1 study of dual-targeting chimeric antigen receptor (CAR)-T cells (EGFR-IL13Rα2 CAR-T cells) ... More

Type I astrocytes and microglia induce a cytokine response in an encephalitic murine coronavirus infection.

Authors:Lavi E, Cong L

Journal:Exp Mol Pathol

PubMed ID:32454103

'The pathogenesis of viral infections involves an immune response by cytokines, causing a deleterious effect on organ function, in addition to tissue destruction due to viral replication. Clinical symptoms and laboratory findings of the human coronavirus disease COVID-19, caused by the novel coronavirus SARS CoV-2, indicate cytokine involvement. Our laboratory ... More