Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate), No-Weigh™ Format

Citations & References (5)

Thermo Scientific™

Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate), No-Weigh™ Format

Name: Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate), No-Weigh™ Format
SKU: 22122

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製品番号（カタログ番号）	数量
22122	1 g
A39268	10 x 2 mg
22322	50 mg

3 オプション

製品番号（カタログ番号） 22122

価格（JPY）

637,800

Each

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数量:

1 g

計量不要フォーマットのThermo Scientific Pierce Sulfo-SMCCは、水溶性のアミン-スルフヒドリルクロスリンカーで、中鎖のシクロヘキサンスペーサーアーム（8.3オングストローム）の一方の末端にNHSエステル反応基を、もう一方の末端にマレイミド反応基を有しています。

計量不要フォーマットのSulfo-SMCCの特長：

•反応基：スルホ-NHSエステルおよびマレイミド
• 反応標的：アミノ基およびスルフヒドリル基
• 水溶性（SMCCとは対照的）であるため、クロスリンクは生理溶液中で可能
• 高純度の結晶試薬を使用して、高純度のマレイミド活性化誘導体を作製可能
• シクロヘキサンクロスリンクはマレイミド基の安定性を向上させるため、Sulfo-SMCCはタンパク質のマレイミド活性化に最適なクロスリンカーです。マレイミド基は、pH 7で4℃の0.1 Mリン酸ナトリウムバッファー中で64時間安定です。

スルホスクシンイミジル-4-（N-マレイミドメチル）シクロヘキサン-1-カルボン酸（スルホ-SMCC）は、切断不可能な膜非透過性クロスリンカーです。アミン反応性N-ヒドロキシスクシンイミド（NHSエステル）とスルフヒドリル反応性マレイミド基を有しています。NHSエステルは、pH 7～9で1級アミンと反応して安定したアミド結合を形成します。マレイミドはpH 6.5～7.5でスルフヒドリル基と反応して、安定したチオエーテル結合を形成します。Sulfo-SMCCとSMCCのマレイミド基は、スペーサーアームにシクロヘキサンクロスリンクが存在するため、pH 7.5まで非常に安定しています。Sulfo-SMCCは親水性のスルホニル部分を有しているため、水や一般的に使用される多くのバッファーに約10 mMまで溶解できます。そのため、タンパク質構造を乱す可能性のある有機溶媒を使用する必要はありません。

アプリケーション：
• 抗体の酵素標識 - 酵素特異性および抗体特異性の両方を保持可能
• 1ステップまたは2ステップのクロスリンク反応で特異的バイオコンジュゲートを作成
• ハプテン結合のためにスルフヒドリル反応性のマレイミド活性化キャリアタンパク質を作成

仕様：
Sulfo-SMCCは、特異性が非常に高いバイオコンジュゲートとマレイミド活性化タンパク質を作成し、データの完全性を確保してクロスリンクにおける最高レベルの一貫性を提供するよう、非常に高度な仕様を満たすよう製造されています。Sulfo-SMCCの各ロットは、以下の最低限の仕様を満たすことが検査されています。

• 同一性：IRスキャンでは、Sulfo-SMCCクロスリンカーの構造および官能基に特有のピークのみが現れる
• 純度：定量的NMRで≥ 90%（クロスリンカー純度では最高水準）
•溶解度：脱イオン水で≥ 10 mg/mL

製品リファレンス：
クロスリンカーアプリケーションガイド -- この製品に関する最新の文献リファレンスを検索してください

関連製品
PPierce™ Premium Grade Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate)
SM(PEG)4 (PEGylated SMCC crosslinker)

研究用途にのみご使用ください。診断目的には使用できません。

仕様

細胞透過性No

概要Sulfo-SMCC

形状Powder

標識法化学的標識

分子量436.37

PEG化No

製品ラインPierce

数量1 g

反応性部分マレイミド、Sulfo-NHSエステル

出荷条件Ambient

溶解性水

スペーサーアーム長8.3 Å

水溶性Yes

化学反応性Amine-Sulfhydryl

CleavableNo

クロスリンカータイプヘテロ三官能性

フォーマットStandard, Single-use, Premium-grade

製品タイプ架橋剤

スペーサー短鎖

Unit SizeEach

組成および保存条件

受け取り後、-20℃で乾燥した場所に保存。製品は室温で出荷されます。

よくあるご質問（FAQ）

Can you provide the shelf-life for Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate)?

Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the shelf life of Sulfo-SMCC (Cat. No. 22322)?

Sulfo-SMCC is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (desiccated at -20 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the difference between SMCC and Sulfo-SMCC?

SMCC is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane-stabilized spacer arm (8.3 angstroms). Sulfo-SMCC is a similar compound but it has a sulfonate group on the NHS ring which makes it water-soluble whereas SMCC is water-insoluble and needs to be dissolved first in an organic solvent like DMF or DMSO. Subsequent dilution into aqueous reaction buffer is generally possible, and most protein reactants will remain soluble if the final concentration of organic solvent is less than 10%.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Is the Thermo Scientific IP Lysis Buffer similar to RIPA buffer?

The Thermo Scientific IP Lysis Buffer (Cat. No. 87787) is a mammalian whole cell lysis buffer based on a modified RIPA buffer formulation without SDS. This moderate strength lysis buffer is able to effectively solubilize cellular proteins but does not liberate genomic DNA or disrupt protein complexes like RIPA buffer.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Is the cyclohexane ring in Sulfo-SMCC crosslinker cis or trans?

The cyclohexane ring in Sulfo-SMCC crosslinker is trans.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate), 1 g FAQs

引用および参考文献 (5)

引用および参考文献

Abstract

Solid phase DNA amplification: characterisation of primer attachment and amplification mechanisms.

Authors:Adessi C, Matton G, Ayala G, Turcatti G, Mermod JJ, Mayer P, Kawashima E

Journal:Nucleic Acids Res

PubMed ID:11024189

Different chemical methods used to attach oligonucleotides by their 5'-end on a glass surface were tested in the framework of solid phase PCR where surface-bound instead of freely-diffusing primers are used to amplify DNA. Each method was first evaluated for its capacity to provide a high surface coverage of oligonucleotides ... More

Substrate-induced conformational change in bacterial complex I.

Authors:Mamedova AA, Holt PJ, Carroll J, Sazanov LA

Journal:J Biol Chem

PubMed ID:15037611

'The mechanism coupling electron transfer and proton pumping in respiratory complex I (NADH-ubiquinone oxidoreductase) has not been established, but it has been suggested that it involves conformational changes. Here, the influence of substrates on the conformation of purified complex I from Escherichia coli was studied by cross-linking and electron microscopy. ... More

Chondrex: new marker of joint disease.

Authors:Harvey S, Weisman M, O'Dell J, Scott T, Krusemeier M, Visor J, Swindlehurst C

Journal:Clin Chem

PubMed ID:9510855

Chondrex, a major secretory protein of human chondrocytes and synovial fibroblasts, is increased in serum of patients with joint and cartilage disease. We have developed a sandwich-type ELISA for quantifying chondrex in serum. The interassay CVs were 2.8-3.7% and the average within-run and total CVs were 3.6% and 5.4%, respectively. ... More

Development of sensitive immunoassays to detect amylin and amylin-like peptides in unextracted plasma.

Authors:Percy AJ, Trainor DA, Rittenhouse J, Phelps J, Koda JE

Journal:Clin Chem

PubMed ID:8605675

Amylin is a 37-amino-acid polypeptide synthesized in and secreted from pancreatic beta cells along with insulin. Its biological actions include the slowing and reduction of postmeal increases in plasma glucose concentrations. Studies of the basic amylin biology in humans have been hampered by the lack of a rapid, sensitive assay ... More

Sulfhydryl-based tumor antigen-carrier protein conjugates stimulate superior antitumor immunity against B cell lymphomas.

Authors:Betting DJ, Kafi K, Abdollahi-Fard A, Hurvitz SA, Timmerman JM

Journal:J Immunol

PubMed ID:18768870

Therapeutic vaccination of B cell lymphoma patients with tumor-specific Ig (idiotype, or Id) chemically coupled to the immunogenic foreign carrier protein keyhole limpet hemocyanin (KLH) using glutaraldehyde has shown promising results in early clinical trials, and phase III trials are underway. However, glutaraldehyde Id-KLH vaccines fail to elicit anti-Id immune ... More