対応範囲:0.5 mL IgGサンプルの精製10回分• MelonゲルIgG精製担体、1.2 mL • Melonゲル精製バッファー、100 mL • Zebaスピン脱塩カラム、7K MWCO、2 mL、10本 • スピンカラム、0.8 mL、10本 • 微量遠心機用チューブ、2 mL、30本
4℃で保管してください。
よくあるご質問(FAQ)
Will glycerol pose a problem when purifying antibodies with the Pierce Antibody Clean-up Kit (Cat. No. 44600)?
When using the Pierce Antibody Clean-up Kit, glycerol may pose a problem for the spin desalting columns for buffer exchange, but not for the Melon Gel itself. If glycerol concentrations are high, the solution becomes viscous enough to exert a frictional drag during spin column chromatography and the performance and yield may suffer.
Please find a technical tip on using Melon Gel for cleaning up BSA or gelatin from commercial antibodies.
If the glycerol concentration is higher than 10%, dialysis may be considered to exchange the sample into the Melon Gel buffer. We have some dialysis devices suitable for small volume dialysis, e.g., the Slide-A-Lyzer MINI devices: see here. Alternatively, some of the smaller Slide-A-Lyzer dialysis cassettes can be used: see here.
I want to use the Antibody Clean-Up Kit (Cat. No. 44600) with a purified antibody that has a "protein based stabilizer". Do I need to first perform the Zeba desalting step A in the kit, or can I proceed directly to the BSA/Gelatin Removal step B?
The Zeba desalting step A is necessary as it results in a buffer exchange into the Melon Gel Purification Buffer. Having the antibody in this buffer is critical for the functionality of the Melon Gel used to remove protein.
I typically use BSA or gelatin to store my purified antibody solution for long-term storage. I'm worried that it will interfere with my immunodetection experiment. Is there a way to remove BSA or gelatin from my antibody solution?
The Pierce Antibody Clean-up Kit (Cat. No. 44600) was specifically designed for removal of amine containing molecules from IgG. Please view this Tech Tip (https://tools.thermofisher.com/content/sfs/brochures/TR0055-Melon-Gel-for-BSA.pdf) for additional strategies on how to remove BSA and gelatin from antibody solutions.