対応範囲:カラムあたり0.1~1 mgのスルフヒドリルペプチドを固定化 • SulfoLinkカラム、2 mL、5 カラム • SulfoLink結合バッファー、120 mL • 洗浄液、120 mL • PBSパック(500 mL作成)、1パック • ボンドブレーカーTCEP溶液、中性pH、0.5 mL • L- システイン·HCl、100 mg • カラムアクセサリー
4℃で遮光された状態で保存。
よくあるご質問(FAQ)
What is the binding capacity of SulfoLink Resin?
SulfoLink Resin can bind approximately 5 mg of reduced IgG per mL of support. For peptides, the capacity is approximately 1 mg of peptide per mL of gel. Capacity for other compounds will vary.
When using the SulfoLink Coupling Resins and Kits, my peptide is not soluble in the coupling buffer. What can I do?
SulfoLink Immobilization is compatible with 3-4 M fresh urea or guanidine. Alternatively, dissolve the peptide in 100% DMSO. Add the peptide in DMSO to the coupling buffer so that the DMSO does not exceed 20% of the final solution.
SulfoLink Resin is crosslinked 6% beaded agarose that has been derivatized with a 12-atom spacer arm that ends in an iodoacetyl group. This spacer arm is helpful for peptides or large proteins that might otherwise be sterically hindered in binding their ligand. The iodoacetyl group reacts with free sulfhydryls at pH 7.5-8.5 to form covalent thioether bonds. The sulfhydryl must be present in the reduced form because disulfides will not react with the iodoacetyl group.