Thermo Scientific™

SulfoLink™ Immobilization Kit for Proteins, 2 mL

Catalog number: 44995
Thermo Scientific™

SulfoLink™ Immobilization Kit for Proteins, 2 mL

Catalog number: 44995
Catalog Number
44995
Unit Size
5 columns
Price (USD)
Price: 347.00
Your Price:
Availability
-
Quantity
Catalog Number
Unit Size
1 column
Price (USD)
Price: 254.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
449955 columns
Price: 347.00
Your Price:
-
203251 column
Price: 254.00
Your Price:
-
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Thermo Scientific SulfoLink Immobilization Kit for Proteins, 2 mL is a kit containing all the reagents for preparing the protein sample for efficient immobilization and five columns containing 2 mL of SulfoLink Resin that can be used in either gravity-flow or centrifuge format for efficient coupling reactions and multiple cycles of affinity purification.

Features of SulfoLink Immobilization Kit for Proteins, 2 mL:

Specific to sulfhydryl (-SH) groups—iodoacetyl groups react specifically with sulhydryls to form irreversible thioether bonds
Fast—spin columns increase protocol speed; prepare and couple protein samples in 3.5 hours
Flexible coupling conditions—use pH 7.5 to 9.0 aqueous buffers, organic solvent (e.g., 20% DMSO) or denaturant (guanidine·HCl), as needed for protein or peptide solubility during coupling reaction
Easy-to-follow instructions—streamlined protocols for sample preparation, immobilization, and affinity purification
High capacity—Immobilize 2 to 20 mg of protein per 2 mL column of SulfoLink Coupling Resin

Applications:
• Immobilize peptides having terminal cysteine residues to purify antibodies that were generated against peptide immunogens prepared by maleimide conjugation
• Immobilize antibodies in oriented manner through hinge-region sulfhydryls to ensure that antigen binding sites are not sterically hindered for antigen affinity purification

When incubated with a solution of protein that contains reduced cysteine residues, the iodoacetyl groups of SulfoLink Resin react specifically and efficiently with the exposed sulfhydryls (-SH) to form covalent and irreversible thioether bonds that permanently attach the protein to the resin. The result is a custom-made affinity resin for purification of antibodies, antigens and other molecules of interest.

Related Products
SulfoLink™ Coupling Resin
SulfoLink™ Immobilization Kit for Peptides, 2 mL
SulfoLink™ Immobilization Trial Kit, 2 mL
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Capacity (Metric)
2 mL
Form
15 mL Conical, Kit, Spin Column
Column Type
Affinity, Agarose Resin
Product Line
SulfoLink™
Quantity
5 Column Kit
Type
Immobilization Kit
Stationary Phase
Haloacetyl
Sufficient For
2 to 20 mg of Antibody per Column
Format
Spin Column

Contents & Storage

Sufficient For: Immobilizing 1 to 10 mg of antibody per column
• SulfoLink Columns, 2 mL, 5 columns
• SulfoLink Preparation Buffer, 7.5 mL
• SulfoLink Coupling Buffer, 500 mL
• Wash Solution, 120 mL
• PBS Pack (makes 500 mL), 1 pack
• 2-Mercaptoethylamine·HCl, 5 x 6 mg
• L-Cysteine·HCl, 100 mg
• Zeba Spin Desalting Columns, 5 mL, 5 columns
• Column Accessories

Store at 4°C protected from light.