Thermo Scientific™

SulfoLink™ Coupling Resin

Catalog number: 20401
Thermo Scientific™

SulfoLink™ Coupling Resin

Catalog number: 20401
Catalog Number
20401
Unit Size
10 mL
Price (USD)
Price: 218.00
Your Price:
Availability
-
Quantity
Catalog Number
Unit Size
50 mL
Price (USD)
Price: 716.00
Your Price:
Availability
-
Quantity
Catalog Number
Unit Size
250 mL
Price (USD)
Price: 2,720.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
2040110 mL
Price: 218.00
Your Price:
-
2040250 mL
Price: 716.00
Your Price:
-
20404250 mL
Price: 2,720.00
Your Price:
-
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Thermo Scientific SulfoLink Coupling Resin is porous, crosslinked, 6% beaded agarose that has been activated with iodoacetyl groups for covalent immobilization of cysteine-peptides and other sulfhydryl molecules.

Features of SulfoLink Coupling Resin:

Specific to sulfhydryl (-SH) groups—iodoacetyl groups react specifically with sulhydryls to form irreversible thioether bonds
Fast—spin columns increase protocol speed; prepare and couple samples in 2 hours (peptides) to 3.5 hours (proteins)
Flexible coupling conditions—use pH 7.5 to 9.0 aqueous buffers, organic solvent (e.g., 20% DMSO) or denaturant (guanidine·HCl), as needed for protein or peptide solubility during coupling reaction
Easy-to-follow instructions—streamlined protocols for sample preparation, immobilization, and affinity purification
High capacity—Immobilize 1 to 2 mg of peptide or 2 to 20 mg of protein per 2 mL column of SulfoLink Coupling Resin

When incubated with a solution of peptide or protein that contains reduced cysteine residues, the iodoacetyl groups of SulfoLink Resin react specifically and efficiently with the exposed sulfhydryls (-SH) to form covalent and irreversible thioether bonds that permanently attach the peptide or protein to the resin. The result is a custom-made affinity resin for purification of antibodies, antigens and other molecules of interest. Choose one of two convenient SulfoLink Immobilization Kits for peptides or proteins. Each kit contains all the reagents for preparing the sample and five columns containing 2 mL of SulfoLink Resin that can be used in either gravity-flow or centrifuge format for efficient coupling reactions and multiple cycles of affinity purification. A single-column Trial Kit for use with either peptides or proteins is also available, or the SulfoLink Coupling Resin can be purchased separately.

Applications:
• Immobilize peptides having terminal cysteine residues to purify antibodies that were generated against peptide immunogens prepared by maleimide conjugation
• Immobilize antibodies in oriented manner through hinge-region sulfhydryls to ensure that antigen binding sites are not sterically hindered for antigen affinity purification

Related Products
SulfoLink™ Immobilization Kit for Peptides, 2 mL
SulfoLink™ Immobilization Kit for Proteins, 2 mL
SulfoLink™ Immobilization Trial Kit, 2 mL
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form
Liquid Suspension
Column Type
Affinity, Agarose Resin
Product Line
SulfoLink™
Quantity
10 mL
Type
Resin
Stationary Phase
Haloacetyl
Sufficient For
1 mg Peptide or 10 mg Antibody per mL of Resin
Format
Bottle

Contents & Storage

Upon receipt store product in the dark at 4°C. Product is shipped at ambient temperature.