LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells

Citations & References (210)

Invitrogen™

LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells

LIVE/DEAD™生存率／細胞毒性キットは、原形質膜の完全性とエステラーゼ活性に基づき集団内の細胞の生存率を判定する、迅速で簡単な2色アッセイです。このキットは、フローサイトメトリー、蛍光顕微鏡、蛍光マイクロプレートリーダーで使用できます。代替手法より優れた利点：• 迅速• 安全•詳細を見る

製品番号（カタログ番号）	数量
L3224	1 kit

製品番号（カタログ番号） L3224

価格（JPY）

130,100

お問い合わせください ›

1 kit

LIVE/DEAD™生存率／細胞毒性キットは、原形質膜の完全性とエステラーゼ活性に基づき集団内の細胞の生存率を判定する、迅速で簡単な2色アッセイです。このキットは、フローサイトメトリー、蛍光顕微鏡、蛍光マイクロプレートリーダーで使用できます。

代替手法より優れた利点：

• 迅速
• 安全
• 高感度
• 低価格

多様な技術や細胞型で簡単に使用可能
遍在的な細胞内エステラーゼ活性、および無傷の原形質膜に基づき、生細胞の特性が識別されます。LIVE/DEAD™生存率／細胞毒性キットは、細胞内エステラーゼ活性を示す緑色蛍光カルセインAMによる染色、原形質膜の完全性消失を示す赤色蛍光エチジウムホモダイマー1による染色を同時に行うことで、生細胞と死細胞とを迅速に区別します。これは、細胞毒性条件によってこれらの細胞効果がもたらされる、ほとんどの真核生物細胞に適合します。このアッセイは、さまざまな蛍光検出法に有用です。

高感度、安全、効率的
LIVE/DEAD™生存率／細胞毒性キットは、生細胞と死細胞の区別に一般的に使用される手法であるトリパンブルー色素排除法より高い感度を有します。LIVE/DEAD™生存率／細胞毒性キットは、生細胞に作用するカルセインAM、死細胞に作用するエチジウムホモダイマー1がいずれも強い蛍光を放出し、高いコスト効果と高感度を実現します。どちらの染色剤も、細胞と相互作用するまでは実質的に無蛍光であるため、バックグラウンドは低レベルです。

幅広いアプリケーションに利用可能なLIVE/DEAD™アッセイ
Invitrogen LIVE/DEAD™生存率アッセイには、哺乳類細胞、細菌、酵母、真菌類に対応するさまざまな種類があります。なお、フローサイトメトリーにおける細胞内染色には、固定可能な死細胞染色キットもご利用いただけます。すべてのLIVE/DEAD™アッセイは、生細胞と死細胞とを迅速かつ確実に区別できます。

関連リンク
• すべてのLIVE/DEAD™アッセイ製品とその詳細をご覧ください。

研究用にのみ使用できます。診断用には使用いただけません。

細胞タイプ哺乳類細胞

概要LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells

検出法蛍光

染色剤タイプその他の標識または色素

フォーマットチューブ、96ウェルプレート、スライド

数量1 kit

出荷条件室温

色Green, Red

Emission517/617

Excitation Wavelength Range494, 528 nm

使用対象（アプリケーション）生存率アッセイ

使用対象 (装置)蛍光顕微鏡, フローサイトメーター, マイクロプレートリーダー

製品ラインLIVE/DEAD

製品タイプViability/Cytotoxicity Kit

Unit SizeEach

組成および保存条件

フリーザー（-5℃～-30℃）に保存し、遮光してください。

よくあるご質問（FAQ）

I need to use a dead cell control for my viability assay. Do you have a protocol for killing cells for this?

Heat killing is commonly used. Place your cells in a tube in buffer and heat at 60^oC for 20 minutes. You can also kill your cells by fixing them with ice cold 70% ethanol for 15 minutes. The ethanol-killed cells can then be stored at -20^oC until needed, at which point you wash out the ethanol and replace with buffer.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am doing a Live/Dead assay using Calcein, AM, for live cells and ethidium homodimer-1 for dead cells. Can I fix the cells after labeling and retain the staining?

This is not recommended. Neither Calcein nor ethidium homodimer-1 bind to any cellular components upon fixation. There is no guarantee that the dyes will be retained upon fixation or any subsequent wash steps. We recommend scoring for live and dead cells as soon as possible after staining.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How should I store the LIVE/DEAD Viability/Cytotoxicity Kit, for mammalian cells (Cat. No. L3224)?

We recommend storing the LIVE/DEAD Viability/Cytotoxicity Kit, for mammalian cells (Cat. No. L3224) in the freezer at -5 degrees C to -30 degrees C and protected from light.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What fluorescent viability assays can I use on the Countess II FL automated cell counter?

We have validated the following kits for use on the Countess II FL Automated Cell Counter:

LIVE/DEAD Viabilty/Cytoxicity Kit (Cat. No. L3224) containing calcein AM and ethidium homodimer-1
ReadyProbes Cell Viability Imaging Kit, Blue/Green (Cat. No. R37609)
ReadyProbes Cell Viability Imaging Kit, Blue/Red (Cat. No. R37610) containing NucBlue Live/NucGreen Dead and NucBlue Live/propidium iodide
See this Application Note for details - https://www.thermofisher.com/content/dam/LifeTech/global/life-sciences/Laboratory%20Instruments/Files/0415/CO014723-Countess-II-FL-Viability-Appnote_FHR.pdf.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How do I prepare dead cell controls for LIVE/DEAD cell viability assays?

There are two easy options. One is to heat-inactivate the cells by placing at 60 degrees C for 20 minutes. The second is to subject the cells to 70% ethanol. Alcohol-fixed cells can be stored indefinitely in the freezer until use, potentially up to several years.

Centrifuge cells, pellet, and remove supernatant.
Fix cells: Add 10 mL ice cold 70% ETOH to a 15 mL tube containing the cell pellet, adding dropwise at first while vortexing, mix well.
Store in freezer until use.
When ready to use, wash twice and resuspend in buffer of choice.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (210)

引用および参考文献

Abstract

Enzymatic isolation and characterization of single vascular smooth muscle cells from cremasteric arterioles.

Authors:Jackson WF,Huebner JM,Rusch NJ

Journal:Microcirculation (New York, N.Y. : 1994)

PubMed ID:9110282

OBJECTIVE: The goal of the present study was to develop a method to isolate viable arteriolar muscle cells from single cremasteric arterioles, which retain the contractile and electrophysiological phenotype of the donor microvessels. METHODS: Arterioles were hand-dissected from rat and hamster cremaster muscles and dissociated by incubation in papain and ... More

Enzymatic isolation and characterization of single vascular smooth muscle cells from cremasteric arterioles.

Authors:Jackson WF,Huebner JM,Rusch NJ

Journal:Microcirculation (New York, N.Y. : 1994)

PubMed ID:8930888

OBJECTIVE: The goal of the present study was to develop a method to isolate enzymatically viable arteriolar muscle cells from single cremasteric arterioles, which retain the contractile and electrophysiological phenotype of the donor microvessels. METHODS: Arterioles were hand-dissected from rat and hamster cremaster muscles and dissociated by incubation in papain ... More

Calcein: a novel marker for lymphocytes which enter lymph nodes.

Authors:Weston SA, Parish CR

Journal:Cytometry

PubMed ID:1451604

Previous studies have identified unique cell surface antigens which are associated with the specific binding of lymphocytes to high endothelial venules (HEV). Evidence is presented in this paper which demonstrates that uptake of the fluorescent dye calcein by lymphocytes represents an additional marker for the lymph node homing subpopulation of ... More

Successful storage of peripheral nerve before transplantation using green tea polyphenol: an experimental study in rats.

Authors:Ikeguchi R, Kakinoki R, Okamoto T, Matsumoto T, Hyon SH, Nakamura T

Journal:Exp Neurol

PubMed ID:14769360

Green tea polyphenol is known to act as a buffer, reducing biological responses to oxidative stress. Several effects of polyphenol have been reported, such as protection of tissue from ischemia, antineoplasmic and anti-inflammatory effects, and suppression of arteriosclerosis. In this study, we investigated whether peripheral nerve segments could be kept ... More

Human stem cell delivery for treatment of large segmental bone defects.

Authors:Dupont KM, Sharma K, Stevens HY, Boerckel JD, García AJ, Guldberg RE,

Journal:Proc Natl Acad Sci U S A

PubMed ID:20133731

'Local or systemic stem cell delivery has the potential to promote repair of a variety of damaged or degenerated tissues. Although various stem cell sources have been investigated for bone repair, few comparative reports exist, and cellular distribution and viability postimplantation remain key issues. In this study, we quantified the ... More

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