Novex™ AP Chemiluminescent Substrate (CDP-Star™)

For colorimetric alkaline phosphatase substrates, we offer the following products:

Novex AP Chromogenic Substrate (BCIP/ NBT) (Cat. No. WP20001)

1-Step NBT/BCIP Substrate Solution (Cat. No. 34042)

1-Step PNPP Substrates Solution (Cat. No. 37621)

NBT and BCIP are also available as standalone products:

NBT Substrate Powder (nitro-blue tetrazolium chloride) (Cat. No. 34035)

BCIP Substrate Powder (5-bromo-4-chloro-3'-indolylphosphate p-toluidine) (Cat. No. 34040)

For chemiluminescent alkaline substrates, we offer the following products:

Novex AP Chemiluminescent Substrate (CDP-Star) (Cat. No. WP20002)

NovaBright Phospha-Light EXP Assay Kit for SEAP (Secreted Placental Alkaline Phosphatase) Reporter Gene Detection (Cat. Nos. N10577, N10578)

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Invitrogen AP Chemiluminescent Substrate (CDP-Star substrate) and Invitrogen ECL Chemiluminescent Substrate are non-radioactive substrates for chemiluminescence-based immunodetection of alkaline phosphatase (AP) or horse radish peroxidase (HRP), respectively, on western blots or dot blots. Invitrogen Chemiluminescent Substrates provide detection sensitivities superior to that of precipitating chromogenic substrates. Low picogram levels of detection can be achieved using either X-ray film or imaging equipment.

Invitrogen AP Chemiluminescent Substrate consists of a ready-to-use solution of CDP-Star substrate, a dioxetane-based substrate for detection of alkaline phosphatase. The Invitrogen AP Chemiluminescent Substrate Enhancer consists of a 20X solution of Nitro-Block-II enhancer that is mixed with the AP Chemiluminescent Substrate when probing blots on nitrocellulose membranes. Do not use the enhancer with PVDF membranes, as it can produce high background. Light emission for this substrate builds rapidly in the first 20 mins after membrane incubation and reaches peak intensity after 45-60 mins. Light emission has a maximal wavelength at 461-466 nm, and continues for several hours and in some cases, for days. Invitrogen AP Chemiluminescent Substrate and Invitrogen AP Chemiluminescent Substrate Enhancer are available as standalone products (Cat. Nos. WP20002 and WP20003) and are also components of the WesternBreeze Chemiluminescent Detection kits.

Invitrogen ECL Chemiluminescent Substrate Reagent Kit is a two-part reagent consisting of a luminol and an enhancer for the detection of horse radish peroxidase. Reagent A and Reagent B are mixed in equal volumes before application to the blot. Light emission for Invitrogen ECL Chemiluminescent Substrate is most intense from 5-30 minutes after membrane incubation and decreases slowly with time over the course of several hours.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Yes, you can purchase them as standalone products and below are the Cat. Nos.:

- Chemiluminescent Substrate, Cat. No. WP20002
- Chemiluminescent Substrate Enhancer, Cat. No. WP20003

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.