peptones-showcase

Enhance your cell culture performance

We offer an extensive portfolio of nutritionally diverse animal origin (AO) and animal origin—free (AOF) peptones to supplement a variety of cell culture media.

  • Alternative to serum – Offers essential nutrients shown to increase performance and production in a variety of vaccine applications.
  • Protective effects – Fortifies cell culture medium to improve cultivation, productivity, and anti-apoptosis properties during scale-up.
  • Performance enhancer – Reduces time to market with high-performance products that meet stringent quality standards and rigorous raw sourcing policies.

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Learn more about Gibco Advanced Bioprocessing products ›

Solutions for bioproduction needs - Mammalian, bacterial, and vaccines

Our peptones are used in the manufacture of more than 150 biopharmaceutical drugs in the human and animal health markets. We have been at the forefront of driving peptone usage in bioproduction and partner with you to provide the best solutions that lead to high-process consistency.

Your requirements

Our solutions

Bioproduction focused release testing

Traditional and specialized testing

Products developed for bioproduction applications

Strict manufacturing controls

Early comprehensive peptone screening

Large library of peptones

Identify key drivers that impact consistent performance

Analytical and key drivers identification (KDI) services

Technical expertise and specialized analytical capabilities for complex formulations

Available data, analytical services and extensive expertise

Peptones can be used across the development phases to commercialization

Peptones in mammalian, microbial fermentation, and insect cell culture

Peptones are widely used to support human and animal health, vaccines, and diagnostics. Peptones provide tailored solutions for specific applications, including monoclonal antibodies (mAbs) and recombinant proteins. Specifically, in the biopharmaceutical industry, the requirement for high-performance processes has prompted a greater focus on cell culture media and process optimization. As a result, improvements in cell culture media, supplements, and feeds have enabled significant advancements including:

  • Increased protein titers, sometimes exceeding 10 grams per liter
  • Cost-effective production levels with desirable protein quality

 

  • Biosimilar characteristics, such as glycosylation and charge variant profiles, that match those of originator molecules
  • Process development that ensures desired target molecule(s)

Rich in amino acids, peptides, vitamins, carbohydrates, nucleosides, minerals, and other components, peptones are well suited for use as supplements and feeds. They provide critical nutritional and environmental support for mammalian cell culture, microbial fermentation in drug production, and recombinant protein production from bacteria and yeast hosts. The advantages of using peptone-containing media are that they are relatively inexpensive, they support a wide variety of growth from a large group of microorganisms, they promote the growth of the more fastidious organisms, they stimulate toxin production, and they routinely produce higher yields.

  • Select a good base medium - more enriched base media yield better results than deficient media
  • Select a wide variety of peptones to evaluate – consider different substrates and multiple products from the same substrate
  • Create a thorough experimental design – test multiple concentrations and individual and blended conditions
  • Characterize the culture throughout the process using analytical techniques - establish baseline data using spent media in multiple runs with multiple lots of peptone
  • Evaluate performance on proliferation, production, and protein quality - monitoring only one attribute is not always predictive of overall performance

Peptones can be incorporated throughout the development process. However, the earlier you add peptones into your process and optimize your process with peptones incorporated, typically, the better your outcomes.  Since all components in base medium can contribute to variability, as could the addition of a supplement, it is important to have an understanding of your medium as you optimize and reduce sources of variability for process consistency.

upstream-bioprocessing

Peptones have a flexible application and can increase performance when used as a feed. Our products have been used in batch, fed-batch and perfusion processes across a number of applications to improve protein production and quality.

peptone-batch-chart

Figure 2. Peptones have a flexible application and when used as a feed can increase performance.

While increasing and maintaining protein titer continues to be a key bioprocessing goal, ensuring acceptable protein quality has become an equally, if not greater, critical process specification. This is especially relevant to the ever-increasing number of biosimilar therapeutics being developed.

It is desirable to achieve a cost-effective level of production, and it is essential that the quality characteristics of biosimilars, such as glycosylation and charge variant profiles, match those of originator molecules. Monitoring product quality, while optimizing media, supplements, and feed strategies, is essential to developing a process that results in the desired target molecule.

When optimizing a medium or feed, peptones should be considered since they can quickly and cost-effectively improve cell performance.

modulating-protein

Figure 3. Peptones can modulate protein quality to help achieve acceptable glycan profiles.

With the addition of one supplement, peptones bring numerous benefits to your cell culture.  Peptones offer versatility through a balanced composition to meet specific requirements of all cell types.

Figure 4. Peptones are versatile and can be used for a broad range of cell types.

Peptone release testing focused for bioproduction needs

Our stringent traditional and specialized testing covers a range of industries. In addition to the traditional testing performed on peptones designed for use in a range of industries, such as the food industry, we also offer specialized testing for the bioproduction market.

Traditional tests

Specialized tests

pH

Loss on drying

Solubility

Solution clarity

Bioburden

Coliforms

Solution appearance

Endotoxin

Amino nitrogen

Total nitrogen

Filterability

Mycoplasma

Ash

Residue on ignition

Osmolality

Cell culture performance

Sodium chloride

Tests specific to use of peptones in a cell culture process

 

Peptone sources and applications

Product

Source

Application

 

 

Mammalian cell culture

Microbial fermentation

Insect cell culture

Difco™ Beef Extract

Bovine (AO)

 

 

Bacto™ Beef Extract, Desiccated

Bovine (AO)

 

 

Gibco Beef Extract Powder

Bovine (AO)

 

 

Biosate™ Peptone

Mixed animal source (AO)

 

 

Bacto™ Casamino Acids

Bovine, casein (AO)

 

 

Bacto™ Casitone

Mixed animal source (AO)

 

 

Bacto™ Peptone

Mixed animal source (AO)

 

Polypeptone™ Peptone

Mixed animal source (AO)

 

Bacto™ Proteose Peptone

Mixed animal source (AO)

 

 

Bacto™ Proteose Peptone No. 3

Porcine (AO)

 

Bacto™ TC Lactalbumin Hydrolysate

Mixed animal source (AO)

Trypticase™ Peptone

Bovine, casein (AO)

 

 

Bacto™ Neopeptone

Mixed animal source (AO)

 

 

Bacto™ Tryptone

Mixed animal source (AO)

 

 

Bacto™ Tryptose

Mixed animal source (AO)

Bacto™ Malt Extract

Barley (AOF)

 

 

Difco™ Soytone

Soy (AOF)

 

Bacto™ TC Yeastolate

Soy (AOF)

Gibco Yeast Extract

Yeast (AOF)

Bacto™ Yeast Extract

Yeast (AOF)

Difco™ Yeast Extract LD (Low Dusting)

Yeast (AOF)

 

 Bacto™ Yeast Extract Technical

Yeast (AOF)

 

Difco™ Yeast Extract UF

Yeast (AOF)

 

Gibco Cotton 100 UF

Cotton (AOF)

 

Gibco Cotton 200 UF

Cotton (AOF)

 

Gibco Soy 100

Soy (AOF)

 

Gibco Wheat 100 UF

Wheat (AOF)

 

Difco™ TC Yeastolate UF

Yeast (AOF)

 

 

Difco™ Phytone UF

Soy (AOF)

 

 

Starter Pak No. 1*

Yeast, soy (AOF)

 

Starter Pak No. 2*

Yeast, soy, mixed animal source (AO/AOF)

 

Starter Pak No. 3*

Yeast, soy, malt (AOF)

 

*Starter Paks are non-GMP and for evaluation use only.

 

 

 

 

With a wide range of components and nutritional buffers, peptones can be incorporated throughout the development process, although we suggest incorporating peptones into the process as early as possible. Peptones offer balanced composition to meet specific requirements of all cell types while increasing specific productivity and promoting increased cell densities.

Enhance performance

Our trusted peptones help customers achieve high performance and process consistency. Peptones increase specific productivity, promote increased cell densities and improve cell performance with higher cell titers and yield.

Specializing in the needs of the biopharmaceutical industry, we deliver reliable, consistent performance.

cho-performance-chart

Figure 5. Peptones are nutrient rich. Increased performance over chemically defined formulations.

Speed to market

Providing a rapid, low cost solution through accelerated media development, peptones can help to strike a balance across multiple bioprocessing requirements and industry challenges. With the addition of just one peptone supplement to your culture, you can design the optimal formulation to achieve performance and quality, while working within limited time parameters. 

peptone-vs-cd-chart

Figure 6. Peptones enable rapid optimization. Achieving process target faster.

Our peptones are manufactured specifically for the biopharma industry and provide high level of consistency through strict manufacturing controls and raw material specifications. Although not completely defined, peptones are well characterized and long-established cell culture supplements and feeds which can enhance cell growth and/or protein titer while helping achieve or maintain desired protein quality.

Consistency and quality

Process consistency is critical. Understanding and controlling variability is essential to having a robust process. All raw materials have variability and it is important to understand where trace contaminants and variability can be introduced.

Our peptones provide a high level of consistency through strict manufacturing controls and raw material specifications. Filing of peptone-containing processes follows the same guidelines as CD medium and agencies require similar data-driven applications.

consistency-quality-chart

Figure 7. Sources of bioproduction variation. Raw material contributions.

Optimization and sustainability

We work with some of the leading pharmaceutical companies to provide products used in the manufacture of more than 150 biopharmaceutical drugs in the human and animal health markets.

Our peptones are proven supplements for enhancing bioproduction applications and continue to be widely used for the production of today’s blockbuster biotherapeutic drugs and the development of the next generation of biopharmaceutical innovations.

optiization-sustainability-chart

Figure 8. Peptones are a powerful optimization tool.

Manufacturing high-quality cell culture media and supplements

As leaders in the cell culture industry, we are focused on designing, developing and manufacturing high-quality products. Our highly trained and skilled associates have many years of experience in scaling and manufacturing cell culture supplements and media. Our experience and expertise improve upstream processes and reduce project timelines allowing us to exceed our customers’ goals and requirements.

Our commitment to quality is achieved through customer-focused continuous improvement and effective quality systems. Our validated methodologies are based upon regulations according to FDA in the United States and EMA in Europe and focus on:

  • Corrective and preventive actions
  • Production and process controls
  • Equipment and facility controls
  • Record, document and change controls
  • Material controls
  • Design controls
  • Raw material sourcing

Our dedicated animal origin-free facility operates under a quality system with considerations of 21 CFR Parts 210 and 211 regulations for the manufacture of finished pharmaceuticals and is ISO 9001 certified.

Peptone manufacture

Most peptones are manufactured similarly, with steps for hydrolysis and downstream processing. Yeast extract manufacture is an exception because it is an autolysate but the basic steps are the same. The figure below outlines the key steps of peptone manufacture: hydrolysis/digestion, centrifugation, filtration, concentration, and drying. Protein and demineralized water are combined to form a thick suspension of protein material in large-capacity digestion vessels, which are stirred continuously throughout the hydrolysis process. The protein suspension is then adjusted to the optimal pH for the specific enzyme chosen for the hydrolysis.

Ultrafiltration

Ultrafiltration (UF) is a membrane filtration process used to separate or concentrate constituents of protein solutions based on molecular weight. We offer several peptone and yeast extract products that are ultra-filtered, using a molecular weight cut off (MWCO) membrane. The result is a retentate containing molecules over the cut off, which may include fats, larger MW polypeptides and proteins, and a permeate that contains salts, sugars, peptides, smaller polypeptides, and other compounds. Additionally, ultrafiltration reduces the presence of endotoxins known to cause illness in humans or negatively impact the production process.

 

Peptones in mammalian, microbial fermentation, and insect cell culture

Peptones are widely used to support human and animal health, vaccines, and diagnostics. Peptones provide tailored solutions for specific applications, including monoclonal antibodies (mAbs) and recombinant proteins. Specifically, in the biopharmaceutical industry, the requirement for high-performance processes has prompted a greater focus on cell culture media and process optimization. As a result, improvements in cell culture media, supplements, and feeds have enabled significant advancements including:

  • Increased protein titers, sometimes exceeding 10 grams per liter
  • Cost-effective production levels with desirable protein quality

 

  • Biosimilar characteristics, such as glycosylation and charge variant profiles, that match those of originator molecules
  • Process development that ensures desired target molecule(s)

Rich in amino acids, peptides, vitamins, carbohydrates, nucleosides, minerals, and other components, peptones are well suited for use as supplements and feeds. They provide critical nutritional and environmental support for mammalian cell culture, microbial fermentation in drug production, and recombinant protein production from bacteria and yeast hosts. The advantages of using peptone-containing media are that they are relatively inexpensive, they support a wide variety of growth from a large group of microorganisms, they promote the growth of the more fastidious organisms, they stimulate toxin production, and they routinely produce higher yields.

  • Select a good base medium - more enriched base media yield better results than deficient media
  • Select a wide variety of peptones to evaluate – consider different substrates and multiple products from the same substrate
  • Create a thorough experimental design – test multiple concentrations and individual and blended conditions
  • Characterize the culture throughout the process using analytical techniques - establish baseline data using spent media in multiple runs with multiple lots of peptone
  • Evaluate performance on proliferation, production, and protein quality - monitoring only one attribute is not always predictive of overall performance

Peptones can be incorporated throughout the development process. However, the earlier you add peptones into your process and optimize your process with peptones incorporated, typically, the better your outcomes.  Since all components in base medium can contribute to variability, as could the addition of a supplement, it is important to have an understanding of your medium as you optimize and reduce sources of variability for process consistency.

upstream-bioprocessing

Peptones have a flexible application and can increase performance when used as a feed. Our products have been used in batch, fed-batch and perfusion processes across a number of applications to improve protein production and quality.

peptone-batch-chart

Figure 2. Peptones have a flexible application and when used as a feed can increase performance.

While increasing and maintaining protein titer continues to be a key bioprocessing goal, ensuring acceptable protein quality has become an equally, if not greater, critical process specification. This is especially relevant to the ever-increasing number of biosimilar therapeutics being developed.

It is desirable to achieve a cost-effective level of production, and it is essential that the quality characteristics of biosimilars, such as glycosylation and charge variant profiles, match those of originator molecules. Monitoring product quality, while optimizing media, supplements, and feed strategies, is essential to developing a process that results in the desired target molecule.

When optimizing a medium or feed, peptones should be considered since they can quickly and cost-effectively improve cell performance.

modulating-protein

Figure 3. Peptones can modulate protein quality to help achieve acceptable glycan profiles.

With the addition of one supplement, peptones bring numerous benefits to your cell culture.  Peptones offer versatility through a balanced composition to meet specific requirements of all cell types.

Figure 4. Peptones are versatile and can be used for a broad range of cell types.

Peptone release testing focused for bioproduction needs

Our stringent traditional and specialized testing covers a range of industries. In addition to the traditional testing performed on peptones designed for use in a range of industries, such as the food industry, we also offer specialized testing for the bioproduction market.

Traditional tests

Specialized tests

pH

Loss on drying

Solubility

Solution clarity

Bioburden

Coliforms

Solution appearance

Endotoxin

Amino nitrogen

Total nitrogen

Filterability

Mycoplasma

Ash

Residue on ignition

Osmolality

Cell culture performance

Sodium chloride

Tests specific to use of peptones in a cell culture process

 

Peptone sources and applications

Product

Source

Application

 

 

Mammalian cell culture

Microbial fermentation

Insect cell culture

Difco™ Beef Extract

Bovine (AO)

 

 

Bacto™ Beef Extract, Desiccated

Bovine (AO)

 

 

Gibco Beef Extract Powder

Bovine (AO)

 

 

Biosate™ Peptone

Mixed animal source (AO)

 

 

Bacto™ Casamino Acids

Bovine, casein (AO)

 

 

Bacto™ Casitone

Mixed animal source (AO)

 

 

Bacto™ Peptone

Mixed animal source (AO)

 

Polypeptone™ Peptone

Mixed animal source (AO)

 

Bacto™ Proteose Peptone

Mixed animal source (AO)

 

 

Bacto™ Proteose Peptone No. 3

Porcine (AO)

 

Bacto™ TC Lactalbumin Hydrolysate

Mixed animal source (AO)

Trypticase™ Peptone

Bovine, casein (AO)

 

 

Bacto™ Neopeptone

Mixed animal source (AO)

 

 

Bacto™ Tryptone

Mixed animal source (AO)

 

 

Bacto™ Tryptose

Mixed animal source (AO)

Bacto™ Malt Extract

Barley (AOF)

 

 

Difco™ Soytone

Soy (AOF)

 

Bacto™ TC Yeastolate

Soy (AOF)

Gibco Yeast Extract

Yeast (AOF)

Bacto™ Yeast Extract

Yeast (AOF)

Difco™ Yeast Extract LD (Low Dusting)

Yeast (AOF)

 

 Bacto™ Yeast Extract Technical

Yeast (AOF)

 

Difco™ Yeast Extract UF

Yeast (AOF)

 

Gibco Cotton 100 UF

Cotton (AOF)

 

Gibco Cotton 200 UF

Cotton (AOF)

 

Gibco Soy 100

Soy (AOF)

 

Gibco Wheat 100 UF

Wheat (AOF)

 

Difco™ TC Yeastolate UF

Yeast (AOF)

 

 

Difco™ Phytone UF

Soy (AOF)

 

 

Starter Pak No. 1*

Yeast, soy (AOF)

 

Starter Pak No. 2*

Yeast, soy, mixed animal source (AO/AOF)

 

Starter Pak No. 3*

Yeast, soy, malt (AOF)

 

*Starter Paks are non-GMP and for evaluation use only.

 

 

 

 

With a wide range of components and nutritional buffers, peptones can be incorporated throughout the development process, although we suggest incorporating peptones into the process as early as possible. Peptones offer balanced composition to meet specific requirements of all cell types while increasing specific productivity and promoting increased cell densities.

Enhance performance

Our trusted peptones help customers achieve high performance and process consistency. Peptones increase specific productivity, promote increased cell densities and improve cell performance with higher cell titers and yield.

Specializing in the needs of the biopharmaceutical industry, we deliver reliable, consistent performance.

cho-performance-chart

Figure 5. Peptones are nutrient rich. Increased performance over chemically defined formulations.

Speed to market

Providing a rapid, low cost solution through accelerated media development, peptones can help to strike a balance across multiple bioprocessing requirements and industry challenges. With the addition of just one peptone supplement to your culture, you can design the optimal formulation to achieve performance and quality, while working within limited time parameters. 

peptone-vs-cd-chart

Figure 6. Peptones enable rapid optimization. Achieving process target faster.

Our peptones are manufactured specifically for the biopharma industry and provide high level of consistency through strict manufacturing controls and raw material specifications. Although not completely defined, peptones are well characterized and long-established cell culture supplements and feeds which can enhance cell growth and/or protein titer while helping achieve or maintain desired protein quality.

Consistency and quality

Process consistency is critical. Understanding and controlling variability is essential to having a robust process. All raw materials have variability and it is important to understand where trace contaminants and variability can be introduced.

Our peptones provide a high level of consistency through strict manufacturing controls and raw material specifications. Filing of peptone-containing processes follows the same guidelines as CD medium and agencies require similar data-driven applications.

consistency-quality-chart

Figure 7. Sources of bioproduction variation. Raw material contributions.

Optimization and sustainability

We work with some of the leading pharmaceutical companies to provide products used in the manufacture of more than 150 biopharmaceutical drugs in the human and animal health markets.

Our peptones are proven supplements for enhancing bioproduction applications and continue to be widely used for the production of today’s blockbuster biotherapeutic drugs and the development of the next generation of biopharmaceutical innovations.

optiization-sustainability-chart

Figure 8. Peptones are a powerful optimization tool.

Manufacturing high-quality cell culture media and supplements

As leaders in the cell culture industry, we are focused on designing, developing and manufacturing high-quality products. Our highly trained and skilled associates have many years of experience in scaling and manufacturing cell culture supplements and media. Our experience and expertise improve upstream processes and reduce project timelines allowing us to exceed our customers’ goals and requirements.

Our commitment to quality is achieved through customer-focused continuous improvement and effective quality systems. Our validated methodologies are based upon regulations according to FDA in the United States and EMA in Europe and focus on:

  • Corrective and preventive actions
  • Production and process controls
  • Equipment and facility controls
  • Record, document and change controls
  • Material controls
  • Design controls
  • Raw material sourcing

Our dedicated animal origin-free facility operates under a quality system with considerations of 21 CFR Parts 210 and 211 regulations for the manufacture of finished pharmaceuticals and is ISO 9001 certified.

Peptone manufacture

Most peptones are manufactured similarly, with steps for hydrolysis and downstream processing. Yeast extract manufacture is an exception because it is an autolysate but the basic steps are the same. The figure below outlines the key steps of peptone manufacture: hydrolysis/digestion, centrifugation, filtration, concentration, and drying. Protein and demineralized water are combined to form a thick suspension of protein material in large-capacity digestion vessels, which are stirred continuously throughout the hydrolysis process. The protein suspension is then adjusted to the optimal pH for the specific enzyme chosen for the hydrolysis.

Ultrafiltration

Ultrafiltration (UF) is a membrane filtration process used to separate or concentrate constituents of protein solutions based on molecular weight. We offer several peptone and yeast extract products that are ultra-filtered, using a molecular weight cut off (MWCO) membrane. The result is a retentate containing molecules over the cut off, which may include fats, larger MW polypeptides and proteins, and a permeate that contains salts, sugars, peptides, smaller polypeptides, and other compounds. Additionally, ultrafiltration reduces the presence of endotoxins known to cause illness in humans or negatively impact the production process.

 

Ordering information

Despite the growing interest in animal origin-free peptone material, peptones from animal proteins remain effective and reliable for many cell culture and bioprocessing applications. Animal origin (AO) peptones are used in reducing or eliminating serum needs in certain media formulations, and commonly promote strong cell growth and toxin titers for vaccine production. AO peptones are derived from reliable and highly regulated raw materials and they provide a well-performing, cost-effective media component or supplement, particularly in processes and applications with less rigorous animal origin requirements.  

  • Tissue-based peptones are proteins from animal sources that have been hydrolyzed into amino acids and peptides. Sources of animal protein for peptones can include various tissues, like muscle or pancreas; these tissues derive from animals highly regulated for human consumption.
  • Milk-based peptones are derived from bovine milk, which is sourced under the same conditions as milk produced for human consumption. Milk is a complex material, consisting of water, lactose, lipids, salts, and proteins. Casein (80%) and whey (20%) are the fundamental protein components in milk.

 

Historically, microbial and mammalian cell lines have been propagated in media containing animal components, including serum. In recent years, media formulators have been moving to eliminate animal-sourced components in media formulations, motivated by BSE/TSE outbreaks in the 1990s. In response, we have developed a broad offering of yeast extracts, soy peptones, and other animal origin-free media and supplements.

Peptones derived from animal origin-free sources provide unique nutritional profiles suitable for both bacterial fermentation and mammalian cell culture applications. Yeast extracts and yeastolates offer a different range of nutritional choices to enhance production. Soy peptone, one of the first successful plant-based peptones to be optimized into cell culture, is processed in several different ways to provide various nutrient mixes to meet different needs. In addition to these more common animal origin-free components, many other plant-based products have been developed, including malt, cotton, and wheat sourced peptones, which offer an additional breadth of supplements with strong and varied performance characteristics.

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