PCR Enzymes & Master Mixes
We offer a wide selection of high-quality PCR enzymes and reagents to meet your research needs. Choose from a variety of enzyme application types, formats, buffers, and dNTP solutions depending on the flexibility needed to perform your experiments. With PCR enzymes you know and trust such as Applied Biosystems AmpliTaq and AmpliTaq Gold and Invitrogen Platinum Taq, AccuPrime, and Platinum SuperFi DNA polymerases, we have what you need for successful PCR.
PCR types
For everyday PCR amplification
| Taq DNA polymerase Learn more |
AmpliTaq DNA polymerase Learn more |
|
|---|---|---|
| Amplicon size | Up to 5 kb | Up to 5 kb |
| Activation time | Immediate | Immediate |
| Product overhang | 3′ A | 3′ A |
| Exonuclease activity | 5′→ 3′ | 5′→ 3′ |
| Master mix/SuperMix format | Colorless | |
| Stand-alone enzyme | Colorless | |
| *Native Taq and recombinantTaq polymerase are identical in terms of their activity, specificity, thermostability, and performance in PCR. Native Taq has been purified from the host, whereas recombinant Taq has been expressed in a bacterial system and purified. | ||
Other PCR enzymes
You can also use these enzymes for everyday PCR amplification:
- Platinum Taq DNA Polymerase
- AmpliTaq Gold DNA Polymerase
- AmpliTaq DNA Polymerase, LD
To increase specificity and yield
| Platinum IITaq Hot-Start DNA polymerase Learn more |
AmpliTaq Gold DNA polymerase Learn more |
AmpliTaq Gold 360 DNA polymerase Learn more |
|
|---|---|---|---|
| Hot-start technology | Antibody | Chemical | Chemical |
| Universal annealing protocol | Yes | No | No |
| Speed | 15 sec/kb | 60 sec/kb | 60 sec/kb |
| Flexible extension step* | Yes | No | No |
| Inhibitor resistance | Yes | No | No |
| Enhanced specificity | Yes | Yes | Yes |
| Amplicon size | Up to 5 kb | Up to 5 kb | Up to 5 kb |
| GC-rich format | Yes | No | Yes |
| Activation time | 30 sec to 2 min | 10 min | 5-10 min |
| Master mix/Super mix format | Colorless Green** |
Colorless | |
| Stand-alone enzyme | Colorless Green*** |
Colorless | Colorless |
| * The extension step can be extended up to 60 sec/kb without the effect of specificity. ** Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels. ***Green buffer available as separate item for use with stand-alone enzyme. |
|||
For when sequence accuracy is critical
| Platinum SuperFi DNA Polymerase Learn more › |
AccuPrime Taq DNA Polymerase High Fidelity Learn more › |
Platinum Taq DNA Polymerase High Fidelity Learn more › |
|
|---|---|---|---|
| Fidelity (vs. Taq) | >100x | 9x | 6x |
| Hot-start for enhanced specificity | ✓ | ✓ | ✓ |
| Amplicon size | up to 20 kb* | up to 20 kb | up to 20 kb |
| Overhang | Blunt | 3′ A/Blunt | 3′ A/Blunt |
| GC-rich format | ✓ | ||
| Master mix/super mix format | Colorless Green** |
Colorless | |
| Stand-alone enzyme | Colorless Green** |
Colorless | Colorless |
| * Amplification of >20 kb fragment sizes is possible (up to 40 kb), but may require additional optimization of reaction conditions and primer design. ** Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels. |
|||
For robust yields of templates longer than 10 kb
| Platinum SuperFi DNA Polymerase Learn more › |
|
|---|---|
| Amplicon size | Up to 20 kb* |
| Fidelity (vs. Taq) | >100x |
| Hot-start for enhanced specificity | ✓ |
| Master mix/SuperMix | Colorless Green** |
| Stand-alone enzyme | Colorless Green** |
| * Amplification of >20 kb fragment sizes is possible (up to 40 kb), but may require additional optimization of reaction conditions and primer design. ** Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels. |
|
To amplify multiple targets in one reaction
| AccuPrime Taq DNA Polymerase System Learn more › |
Platinum SuperFi DNA Polymerase Learn more › |
Platinum Multiplex PCR Master Mix Learn more › |
|
|---|---|---|---|
| No. of amplicons in single reaction | Up to 15-plex | Up to 15-plex | Up to 20-plex |
| Amplicon size | Up to 5 kb | Up to 2.5 kb | Up to 2.5 kb |
| Hot-start for enhanced specificity | ✓ | ✓ | ✓ |
| Fidelity (vs. Taq) | 2x | >100x | 1x |
| Master mix/super mix format | Colorless (SuperMix I) Colorless (SuperMix II) |
Colorless Green* |
Colorless |
| Stand-alone enzyme | Colorless | Colorless Green* |
|
| * Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels. | |||
Other reagents for multiplex PCR
For everyday PCR amplification
| Taq DNA polymerase Learn more |
AmpliTaq DNA polymerase Learn more |
|
|---|---|---|
| Amplicon size | Up to 5 kb | Up to 5 kb |
| Activation time | Immediate | Immediate |
| Product overhang | 3′ A | 3′ A |
| Exonuclease activity | 5′→ 3′ | 5′→ 3′ |
| Master mix/SuperMix format | Colorless | |
| Stand-alone enzyme | Colorless | |
| *Native Taq and recombinantTaq polymerase are identical in terms of their activity, specificity, thermostability, and performance in PCR. Native Taq has been purified from the host, whereas recombinant Taq has been expressed in a bacterial system and purified. | ||
Other PCR enzymes
You can also use these enzymes for everyday PCR amplification:
- Platinum Taq DNA Polymerase
- AmpliTaq Gold DNA Polymerase
- AmpliTaq DNA Polymerase, LD
To increase specificity and yield
| Platinum IITaq Hot-Start DNA polymerase Learn more |
AmpliTaq Gold DNA polymerase Learn more |
AmpliTaq Gold 360 DNA polymerase Learn more |
|
|---|---|---|---|
| Hot-start technology | Antibody | Chemical | Chemical |
| Universal annealing protocol | Yes | No | No |
| Speed | 15 sec/kb | 60 sec/kb | 60 sec/kb |
| Flexible extension step* | Yes | No | No |
| Inhibitor resistance | Yes | No | No |
| Enhanced specificity | Yes | Yes | Yes |
| Amplicon size | Up to 5 kb | Up to 5 kb | Up to 5 kb |
| GC-rich format | Yes | No | Yes |
| Activation time | 30 sec to 2 min | 10 min | 5-10 min |
| Master mix/Super mix format | Colorless Green** |
Colorless | |
| Stand-alone enzyme | Colorless Green*** |
Colorless | Colorless |
| * The extension step can be extended up to 60 sec/kb without the effect of specificity. ** Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels. ***Green buffer available as separate item for use with stand-alone enzyme. |
|||
For when sequence accuracy is critical
| Platinum SuperFi DNA Polymerase Learn more › |
AccuPrime Taq DNA Polymerase High Fidelity Learn more › |
Platinum Taq DNA Polymerase High Fidelity Learn more › |
|
|---|---|---|---|
| Fidelity (vs. Taq) | >100x | 9x | 6x |
| Hot-start for enhanced specificity | ✓ | ✓ | ✓ |
| Amplicon size | up to 20 kb* | up to 20 kb | up to 20 kb |
| Overhang | Blunt | 3′ A/Blunt | 3′ A/Blunt |
| GC-rich format | ✓ | ||
| Master mix/super mix format | Colorless Green** |
Colorless | |
| Stand-alone enzyme | Colorless Green** |
Colorless | Colorless |
| * Amplification of >20 kb fragment sizes is possible (up to 40 kb), but may require additional optimization of reaction conditions and primer design. ** Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels. |
|||
For robust yields of templates longer than 10 kb
| Platinum SuperFi DNA Polymerase Learn more › |
|
|---|---|
| Amplicon size | Up to 20 kb* |
| Fidelity (vs. Taq) | >100x |
| Hot-start for enhanced specificity | ✓ |
| Master mix/SuperMix | Colorless Green** |
| Stand-alone enzyme | Colorless Green** |
| * Amplification of >20 kb fragment sizes is possible (up to 40 kb), but may require additional optimization of reaction conditions and primer design. ** Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels. |
|
To amplify multiple targets in one reaction
| AccuPrime Taq DNA Polymerase System Learn more › |
Platinum SuperFi DNA Polymerase Learn more › |
Platinum Multiplex PCR Master Mix Learn more › |
|
|---|---|---|---|
| No. of amplicons in single reaction | Up to 15-plex | Up to 15-plex | Up to 20-plex |
| Amplicon size | Up to 5 kb | Up to 2.5 kb | Up to 2.5 kb |
| Hot-start for enhanced specificity | ✓ | ✓ | ✓ |
| Fidelity (vs. Taq) | 2x | >100x | 1x |
| Master mix/super mix format | Colorless (SuperMix I) Colorless (SuperMix II) |
Colorless Green* |
Colorless |
| Stand-alone enzyme | Colorless | Colorless Green* |
|
| * Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels. | |||
Other reagents for multiplex PCR
Need DNA-free DNA polymerases for microbial research or sensitive PCR-based assays? Find out more
Videos
Resources
Support
For Research Use Only. Not for use in diagnostic procedures.
