Invitrogen Quant-iT assay kits utilize advanced fluorophores that become fluorescent upon binding to DNA, RNA, or proteins; the fluorescence intensity of the resulting complex depends directly on the amount of the target molecule in the sample.

  • Accuracy—Quant-iT technology gives more accurate results than can be obtained with UV absorbance readings, because the Quant-iT reagents only report the molecule of interest—there’s no interference from free nucleotides or other contaminants.
  • Sensitivity—Upon binding to DNA, RNA, or protein, the fluorescence of the Quant-iT dyes increases several hundred fold , giving a very high signal-to-background ratio. High signal and low background mean exceedingly high sensitivity—up to 1000 times more sensitive than absorbance readings.
  • Confidence—Confidence comes from knowing your assay is sensitive and accurate enough to quantitate even the most dilute or low-abundance samples, while still leaving enough of your biomolecule for successful downstream applications. With Quant-iT technology, you get the crucial quantitation data you need, without wasting your sample.

Which assay kit is right for me?

Expertly detect fluorescence with Thermo Scientific plate readers

High-sensitivity fluorescence detection of nucleic acids or protein for 96-1536 samples can be quickly performed on Fluoroskan or Fluoroskan FL Microplate Fluorometer or Varioskan LUX Multimode Microplate Reader using Invitrogen reagents for optimal detection. Take advantage of automatic dynamic range selection to get optimal gain settings for each individual well and automation capabilities for even higher throughput.

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Featured application: Sensitive DNA quantification

Read about detection limit (LOD) as low as 0.04ng of DNA in this featured application note highlighting sensitive fluorescence detection of DNA capabilities when you combine Quant-iT technology with a Fluoroskan plate reader.

 Read more about sensitive DNA quantification

Quant-iT assay kits and reagents for use with microplate readers*—for 20–2,000 samples

Product Target Useful range † Ex/Em ‡ No. of assays Cat. No.
Quant-iT dsDNA High-Sensitivity (HS) Assay Kit
dsDNA 0.2–100 ng 510/527 1,000 Q33120
Quant-iT dsDNA Broad-Range (BR) Assay Kit
dsDNA 2–1,000 ng 510/527 1,000 Q33130
Quant-iT RNA Assay Kit
Quant-iT RNA Assay Reagent
RNA 5–100 ng 644/673 1,000 Q33140
Quant-iT RNA Broad-Range BR Assay Kit
RNA 20–1,000 ng 644/63 1,000 Q10213
Quant-iT microRNA Assay Kit
Quant-iT microRNA Reagent
microRNA 1–500 ng 498/518 1,000 Q32882
Quant-iT Protein Assay Kit
Protein 0.25–5 µg 470/570 1,000 Q33210

Quant-iT bulk reagents and kits for >2,000 samples

Product Target Useful range † Ex/Em ‡ No. of assays Cat. No.
Quant-iT PicoGreen dsDNA Assay Kit
Quant-iT PicoGreen dsDNA Reagent

dsDNA 0.2–100 ng 502/523 2,000 P7589
Quant-iT PicoGreen dsDNA Assay Kit
Quant-iT PicoGreen dsDNA Reagent
dsDNA 0.2–100 ng 502/523 2,000 P11496
Quant-iT OliGreen ssDNA Assay Kit
Quant-iT OliGreen ssDNA Reagent
ssDNA 1–200 ng 498/518 2,000 O11492
Quant-iT RiboGreen RNA Assay Kit
Quant-iT RiboGreen RNA Reagent
RNA 20–1,000 ng 500/525 2,000 R11490

* Invitrogen Quant-iT assays and reagents can also be used with the Invitrogen Qubit Fluorometer. †The useful range assumes a 1–20 µL sample volume in a 96-well microplate assay. ‡ Excitation (Ex) and emission (Em), in nm, of the Quant-iT detection reagent. 

Qubit assays for use with the Qubit fluorometers

For everyday needs when quantitating 1–20 samples, use Invitrogen Qubit Assays for use with the Qubit fluorometers. Ideal for cloning, sequencing, transfection, qPCR, microarrays, protein gels, Western blots and protein activity assays. See how easy it is to use the latest version of the Qubit - the Qubit 4 fluorometer.

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