Quant-iT™ RNA HS Reagent

Catalog number: Q32884

Invitrogen™  Related applications: Nucleic Acid Quantitation

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Description

The Quant-iT™ RNA HS Reagent makes RNA quantitation easy and accurate, with a linear detection range of 5–100 ng in sample volumes of 1–20 µl and selectivity for RNA, even in the presence of an equal mass of DNA. Using the protocol provided, this concentrated assay reagent provides sufficient material to perform at least 2000 assays in a 96-well microplate format. The assay is performed at room temperature, and the signal is stable for approximately 3 hours. Common contaminants, such as salts, solvents, detergents, and protein are well tolerated in the assay.

Important Features of Quant-iT™ RNA HS Reagent:

• Accurately detects as little as 2 ng RNA in a microplate well
• Core dynamic range of 5–100 ng
• Approximate fluorescence excitation/emission maxima of 644/673 nm

Additional Options for RNA Quantitation:

• For 1–20 samples: use Qubit® RNA Assay Kit with the Qubit® Fluorometer
• For 20–2,000 samples: use Quant-iT™ RNA Assay Kit with fluorescent microplate readers

Discover the Qubit® Fluorometer
The Qubit® 2.0 Fluorometer is Life Technologies’ second generation fluorometer designed to work seamlessly with our highly specific and sensitive Qubit® quantitation assays as well as the assays you create with the new MyQubit feature.

Introducing MyQubit
New firmware called "MyQubit" allows you to create applications in minutes using parameters that can easily be uploaded to the instrument using a USB drive, without changing the existing assays. All new Qubit® 2.0 instruments are pre-loaded with the new MyQubit firmware, and existing owners can download the firmware here: V3.10 firmware download. Any reagent or assay that is spectrally compatible with the Qubit® hardware can be adapted for use with the Qubit® 2.0 Fluorometer. Additionally we have developed assays that are ready to download, including glucose and cholesterol assays for research and drug discovery applications.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): RNA, FFPE & Fixed Samples
For Use With (Equipment): Microplate Reader, Fluorometer
Product Size: 1 mL
Sample Type (Specific): RNA (>500 bases), Total RNA (Whole Blood), Total RNA (Bacterial), mRNA (Bacterial), Total RNA (Plant), Total RNA (Yeast), mRNA, Total RNA, Tissue (FFPE), Total RNA (Mammalian Host-Bacterial RNA Mixture)

Contents & storage

Store in refrigerator (2–8°C) and protect from light.

Documents

Manuals & protocols

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