Invitrogen™

Anza™ 1 NotI

Catalog number:  IVGN0014

Related applications:

Restriction Enzyme Cloning

Back to top

Description

5'  G  C ↓G  G  C  C  G  C   3' 
3'  C  G  C  C  G  G ↑C  G   5' 

Invitrogen™ Anza™ 1 NotI is a restriction enzyme that cuts DNA at this recognition site: GC^GGCCGC, completely digesting the DNA in 15 minutes at 37°C. Isoschizomers include: CciNI.

For superior convenience, a single buffer and protocol are used with all Anza™ restriction enzymes. The single Anza buffer allows multiple restriction enzymes to be used simultaneously in one reaction. This helps eliminate the need for sequential digests and the DNA clean-up necessary when changing buffers. Additionally, the Anza buffer and restriction enzymes have been designed for flexibility in digestion times. Digestions of up to 16 hours can be carried out without star activity.

Convenient—all Anza enzymes are 100% functional in the single Anza buffer
Fast—complete digestion in 15 minutes
Flexible—overnight digestion without star activity
Simple—a single digestion protocol for all DNA types
Complete system—incorporates Anza DNA Modifying Enzymes for a complete cloning experience

Anza DNA Modifying Enzymes include the Anza T4 DNA Ligase Master Mix, Anza Alkaline Phosphatase Kit, Anza T4 PNK Kit, Anza DNA Blunt End Kit, and Anza DNA End Repair Kit. Use these enzymes for a complete cloning workflow.

Anza Red Buffer, which contains a density gradient and tracking dyes, is supplied for added convenience. Digestion reactions are as efficient in the Anza Red Buffer as in the Anza Buffer. Use of the Anza Red Buffer helps reduce pipetting steps when preparing multiple digestion reactions and a subsequent step includes agarose gel electrophoresis. For applications that require product analysis by fluorescence excitation, the colorless Anza Buffer without the tracking dyes is recommended.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Quantity: 500U
Shipping Condition: Approved for shipment on Wet or Dry Ice
Product Line: Anza™
Methylation Sensitivity: Not dam methylation-sensitive, Not dcm methylation-sensitive, CpG methylation-sensitive
Enzyme: Not I
Compatible Buffer: Anza™ 10x Red Buffer, Anza™ 10x Buffer
Sensitive to Heat Inactivation: Yes
Optimal Reaction Temperature: 37°C
Type IIS RE: No

Contents & storage

25 µL Anza 1 NotI
500 µL Anza Buffer (10X)
500 µL Anza Red Buffer (10X)

Store at -20°C.