GeneChip™ Eukaryotic Poly-A RNA Control Kit

GeneChip™ Poly-A RNA Control Kit は、Prokaryotic Target Labeling Assay詳細を見る

製品番号（カタログ番号）	数量
900433	1 kit

製品番号（カタログ番号） 900433

価格（JPY）

55,300

お問い合わせください ›

1 kit

GeneChip™ Poly-A RNA Control Kit は、Prokaryotic Target Labeling Assay や原核生物用の GeneChip Brand Arrays を利用する 3' 発現研究のための Prokaryotic Target Labeling Assay に使用します。このアッセイは逆転写酵素とランダムヘキサマーのプライマーを利用し、RNA と相補的な DNA を産生します。この cDNA 生成物は DNase I により断片化され、3' 末端を末端トランスフェラーゼでラベル化、GeneChip DNA Labeling Reagent によりビオチン化されます。

当社は原核生物の遺伝子発現研究には、GeneChip Brand Arrays で機能的に検査された Prokaryotic Target Labeling Assay および関連試薬を使用することを推奨しています。

Prokaryotic Target Labeling Assay の同梱物：
• B2 Control Oligo (3nM)
• GeneChip DNA Labeling Reagent
• GeneChip Poly-A RNA Control Kit

Related Links
GeneChip™ Wash Buffer A
GeneChip™ Wash Buffer B

研究用途にのみご使用ください。診断目的には使用できません。

製品タイプOligonucleotides, Primers

数量1 kit

Primerランダムヘキサマー

製品ラインGeneChip

Unit SizeEach

よくあるご質問（FAQ）

What are the estimated Poly-A RNA Control final concentrations when processing expression arrays?

Here are the Poly-A RNA Controls final concentrations (in samples):
- lys 1:100,000
- phe 1:50,000
- thr 1:25,000
- dap 1:7,500

What is the shelf life of the Poly-A RNA Control's first dilution during processing of expression microarrays?

The first dilution of the Poly-A RNA Control can be stored at -20 degrees C for 6 weeks (with up to 4 freeze/thaws).

Can I purchase one, or all, of the four Poly-A Controls separately?

These four controls are only available as a pre-mixed cocktail with the defined concentrations and relative ratios. This combination of concentrations has been tested to provide qualitative information on both sensitivity and dynamic range of the labeling assay. Providing these controls in a pre-mixed format increases ease-of-use and reduces the number of manual dilution steps.
Information on ordering individual poly-A control clones and preparing poly-A stocks is available in Section 2, Chapter 2, and Section 3, Chapter 2, of the GeneChip Expression Analysis Technical Manual.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Can I purchase the Poly-A Control Stock and the Poly-A Control Dil Buffer separately?

The Control Stock and the Dilution Buffer are packaged in the same Kit to provide convenience to customers. Currently they cannot be purchased as separate products.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Do you have any specific recommendation on the type of non-stick tubes to use for diluting the Poly-A RNA Control Stock?

The following tubes were used in-house during product development of this Poly-A RNA Control Kit, and we have not tested any other tubes at this time:
Non-stick RNase-free 0.5 mL microfuge tubes, Ambion, Cat. No. 12350
Non-stick RNase-free 1.5 mL microfuge tubes, Ambion, Cat. No. 12450

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

View all GeneChip™ Eukaryotic Poly-A RNA Control Kit FAQs