Maxima SYBR Green/ROX qPCR Master Mix (2X)
Maxima SYBR Green/ROX qPCR Master Mix (2X)
Thermo Scientific™

Maxima SYBR Green/ROX qPCR Master Mix (2X)

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製品番号(カタログ番号)反応数
K0221200 reactions
K02221,000 Reactions
K02234,000 Reactions
製品番号(カタログ番号) K0221
価格(JPY)
48,300
Each
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反応数:
200 reactions
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Thermo Scientific Maxima SYBR Green qPCR Master Mix is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG) (1). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most most real-time thermal cyclers (see Resources for a compatibility chart).

Highlights

SpecificityMaxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity—detects low copy number targets
Wide linear range—accurate quantification across 9 orders of magnitude
Reproducibility and convenience—ready-to-use 2x master mix

Applications

• Gene expression
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Maxima SYBR Green/ROX qPCR Master Mix (2x) includes Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer supplemented with ROX passive reference dye. Supplied with nuclease-free water.

Related Products
Maxima SYBR Green/ROX qPCR Master Mix (2X)
Maxima SYBR Green/Fluorescein qPCR Master Mix (2X)
Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial
研究用途にのみご使用ください。診断目的には使用できません。
仕様
使用対象 (装置)7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
フォーマットTube
反応数200 reactions
ポリメラーゼTaq DNA Polymerase
製品ラインMaxima
製品タイプReal Time PCR SYBR Master Mix
数量200 reactions
サンプルタイプDNA, dsDNA
対応可能対象200 Reactions of 25 μL
容量2 x 1.25 mL
濃度2X
検出法SYBR
使用対象(アプリケーション)遺伝子発現解析
GC-Rich PCR Performance
PCR法qPCR
反応速度スタンダード
Unit SizeEach
組成および保存条件
Contains:
• 2 x 1.25 mL Maxima SYBR Green/ROX qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase, dNTPs (also dUTP) and SYBR Green I in an optimized PCR buffer with ROX passive reference dye; sufficient for 200 x 25 μL reactions.
• 2 x 1.25 mL Nuclease-Free Water

Store at -20°C.

よくあるご質問(FAQ)

Is the Maxima SYBR Green/ROX qPCR Master Mix (2X) (Cat. No. K0223, K0221, K0222) compatible with the ViiA 7 Real-Time PCR System (Cat. No. 4453542, 4453539)

No, the ViiA 7 Real-Time PCR System (Cat. No. 4453542, 4453539) is not compatible with the Maxima SYBR Green/ROX qPCR Master Mix (2X) (Cat. No. K0223).

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.