Quant-iT Technology
Invitrogen Quant-iT assay kits utilize advanced fluorophores that become fluorescent upon binding to DNA, RNA, or proteins; the fluorescence intensity of the resulting complex depends directly on the amount of the target molecule in the sample.
- Accuracy—Quant-iT technology gives more accurate results than can be obtained with UV absorbance
readings, because the Quant-iT reagents only report the molecule of interest—there’s no interference from free nucleotides or other contaminants. - Sensitivity—Upon binding to DNA, RNA, or protein, the fluorescence of the Quant-iT dyes increases several hundred
fold , giving a very high signal-to-background ratio. High signal and low background mean exceedingly high sensitivity—up to 1000 times more sensitive than absorbance readings.
- Confidence—Confidence comes from knowing your assay is sensitive and accurate enough to quantitate even the most dilute or low-abundance samples, while still leaving enough of your biomolecule for successful downstream applications. With Quant-iT technology, you get the crucial quantitation data you need, without wasting your sample.
Which assay kit is right for me?
| 1–20 samples | 20–2,000 samples | > 2,000 samples |
|---|---|---|
| Qubit assay kits for use with the Qubit Fluorometers | Quant-iT assay kits and reagents for use with microplate readers* | Quant-iT bulk reagents and kits |
Expertly detect fluorescence with Thermo Scientific plate readers
High-sensitivity fluorescence detection of nucleic acids or protein for 96-1536 samples can be quickly performed on Fluoroskan or Fluoroskan FL Microplate Fluorometer or Varioskan LUX Multimode Microplate Reader using Invitrogen reagents for optimal detection. Take advantage of automatic dynamic range selection to get optimal gain settings for each individual well and automation capabilities for even higher throughput.
Featured application: Sensitive DNA quantification
Read about detection limit (LOD) as low as 0.04ng of DNA in this featured application note highlighting sensitive fluorescence detection of DNA capabilities when you combine Quant-iT technology with a Fluoroskan plate reader.
Quant-iT assay kits and reagents for use with microplate readers*—for 20–2,000 samples
| Product | Target | Useful range † | Ex/Em ‡ | No. of assays | Cat. No. |
|---|---|---|---|---|---|
| Quant-iT dsDNA High-Sensitivity (HS) Assay Kit |
dsDNA | 0.2–100 ng | 510/527 | 1,000 | Q33120 |
| Quant-iT dsDNA Broad-Range (BR) Assay Kit |
dsDNA | 2–1,000 ng | 510/527 | 1,000 | Q33130 |
| Quant-iT RNA Assay Kit Quant-iT RNA Assay Reagent |
RNA | 5–100 ng | 644/673 | 1,000 | Q33140 Q32884 |
| Quant-iT RNA Broad-Range BR Assay Kit |
RNA | 20–1,000 ng | 644/63 | 1,000 | Q10213 |
| Quant-iT microRNA Assay Kit Quant-iT microRNA Reagent |
microRNA | 1–500 ng | 498/518 | 1,000 | Q32882 Q32883 |
| Quant-iT Protein Assay Kit |
Protein | 0.25–5 µg | 470/570 | 1,000 | Q33210 |
Quant-iT bulk reagents and kits for >2,000 samples
| Product | Target | Useful range † | Ex/Em ‡ | No. of assays | Cat. No. |
|---|---|---|---|---|---|
| Quant-iT PicoGreen dsDNA Assay Kit Quant-iT PicoGreen dsDNA Reagent |
dsDNA | 0.2–100 ng | 502/523 | 2,000 | P7589 P7581 |
| Quant-iT PicoGreen dsDNA Assay Kit Quant-iT PicoGreen dsDNA Reagent |
dsDNA | 0.2–100 ng | 502/523 | 2,000 | P11496 P11495 |
| Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent |
ssDNA | 1–200 ng | 498/518 | 2,000 | O11492 O7582 |
| Quant-iT RiboGreen RNA Assay Kit Quant-iT RiboGreen RNA Reagent |
RNA | 20–1,000 ng | 500/525 | 2,000 | R11490 R11491 |
* Invitrogen Quant-iT assays and reagents can also be used with the Invitrogen Qubit Fluorometer. †The useful range assumes a 1–20 µL sample volume in a 96-well microplate assay. ‡ Excitation (Ex) and emission (Em), in nm, of the Quant-iT detection reagent.
Qubit assays for use with the Qubit fluorometers
For everyday needs when quantitating 1–20 samples, use Invitrogen Qubit Assays for use with the Qubit fluorometers. Ideal for cloning, sequencing, transfection, qPCR, microarrays, protein gels, Western blots and protein activity assays. See how easy it is to use the latest version of the Qubit - the Qubit 4 fluorometer.
For Research Use Only. Not for use in diagnostic procedures.
