Quant-iT Technology

Invitrogen Quant-iT assay kits utilize advanced fluorophores that become fluorescent upon binding to DNA, RNA, or proteins; the fluorescence intensity of the resulting complex depends directly on the amount of the target molecule in the sample and can help:

• Accuracy—Quant-iT technology gives more accurate results than can be obtained with UV absorbance readings, because the Quant-iT reagents only report the molecule of interest—there’s no interference from free nucleotides or other contaminants.
• Sensitivity—Upon binding to DNA, RNA, or protein, the fluorescence of the Quant-iT dyes increases several hundred fold , giving a very high signal-to-background ratio. High signal and low background mean exceedingly high sensitivity—up to 1000 times more sensitive than absorbance readings.
  
• Confidence—Confidence comes from knowing your assay is sensitive and accurate enough to quantitate even the most dilute or low-abundance samples, while still leaving enough of your biomolecule for successful downstream applications. With Quant-iT technology, you can get the crucial quantitation data you need, without wasting your sample.

Which assay kit is right for me?

Quant-iT assay kits and reagents for use with microplate readers*—for 20–2,000 samples

Quant-iT bulk reagents and kits for >2,000 samples

* Invitrogen Quant-iT assays and reagents can also be used with the Invitrogen Qubit Fluorometer. †The useful range assumes a 1–20 µL sample volume in a 96-well microplate assay. ‡ Excitation (Ex) and emission (Em), in nm, of the Quant-iT detection reagent.