Invitrogen™ Quant-iT™ assay kits utilize advanced fluorophores that become fluorescent upon binding to DNA, RNA, or proteins; the fluorescence intensity of the resulting complex depends directly on the amount of the target molecule in the sample.

  • Accuracy—Quant-iT technology gives more accurate results than can be obtained with UV absorbance readings, because the Quant-iT reagents only report the molecule of interest—there’s no interference from free nucleotides or other contaminants.
  • Sensitivity—Upon binding to DNA, RNA, or protein, the fluorescence of the Quant-iT dyes increases several hundred fold , giving a very high signal-to-background ratio. High signal and low background mean exceedingly high sensitivity—up to 1000 times more sensitive than absorbance readings.
  • Confidence—Confidence comes from knowing your assay is sensitive and accurate enough to quantitate even the most dilute or low-abundance samples, while still leaving enough of your biomolecule for successful downstream applications. With Quant-iT technology, you get the crucial quantitation data you need, without wasting your sample.

Which assay kit is right for me?

Qubit assays for use with the Qubit 3.0 Fluorometer

For everyday needs when quantitating 1–20 samples, use Invitrogen™ Qubit ™ Assays for use with the Qubit 3.0 Fluorometer. Ideal for cloning, sequencing, transfection, qPCR, microarrays, protein gels, Western blots and protein activity assays. These kits are formulated for use with the Qubit 3.0 Fluorometer. Also available is the Qubit 3.0 Quantitation Starter Kit, which includes the Qubit 3.0 Fluorometer and four Qubit Assay Kits.

Quant-iT assay kits and reagents for use with microplate readers*—for 20–2,000 samples

Product Target Useful range † Ex/Em ‡ No. of assays Cat. No.
Quant-iT™ dsDNA High-Sensitivity (HS) Assay Kit
dsDNA 0.2–100 ng 510/527 1,000 Q33120
Quant-iT™ dsDNA Broad-Range (BR) Assay Kit
dsDNA 2–1,000 ng 510/527 1,000 Q33130
Quant-iT™ RNA Assay Kit
Quant-iT™ RNA Assay Reagent
RNA 5–100 ng 644/673 1,000 Q33140
Quant-iT™ RNA Broad-Range BR Assay Kit
RNA 20–1,000 ng 644/63 1,000 Q10213
Quant-iT™ microRNA Assay Kit
Quant-iT™ microRNA Reagent
microRNA 1–500 ng 498/518 1,000 Q32882
Quant-iT™ Protein Assay Kit
Protein 0.25–5 µg 470/570 1,000 Q33210

Quant-iT™ bulk reagents and kits for >2,000 samples

* Invitrogen™ Quant-iT™ assays and reagents can also be used with the Invitrogen™ Qubit™ Fluorometer. †The useful range assumes a 1–20 µL sample volume in a 96-well microplate assay. ‡ Excitation (Ex) and emission (Em), in nm, of the Quant-iT detection reagent.