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Choose from a variety of PCR enzymes and reagents for your applications, with the flexibility needed to perform your experiments. With PCR enzymes you know and trust such as Applied Biosystems AmpliTaq and AmpliTaq Gold, and Invitrogen Platinum II Taq and Platinum SuperFi II DNA polymerases, we have what it takes for successful PCR.

PCR types

To increase specificity and yield

  Platinum II Taq
Hot-Start DNA Polymerase

AmpliTaq Gold 360 DNA Polymerase
Hot-start technology Antibody Chemical
Universal annealing protocol Yes No
Speed 15 sec/kb 60 sec/kb
Flexible extension step* Yes No
Inhibitor tolerance Yes No
Enhanced specificity Yes Yes
Amplicon size Up to 5 kb Up to 5 kb
GC-rich format Yes Yes
Activation time 0.5–2 min 5–10 min
Master mix format Colorless
Green**
Colorless
Stand-alone enzyme Colorless
Green***
Colorless
* The extension step can be extended up to 60 sec/kb without the effect of specificity.
** Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels.
*** Green buffer available as separate item for use with stand-alone enzyme.

For when sequence accuracy is critical

  Platinum SuperFi II DNA Polymerase
Platinum Taq DNA Polymerase High Fidelity
Fidelity vs. Taq enzyme >300x 6x
Hot start for enhanced specificity Yes Yes
Universal annealing temperature at 60°C Yes No
Amplicon size Up to 20 kb* Up to 20 kb
Overhang Blunt 3′ A/Blunt
GC-rich amplification Yes
No
Master mix format Colorless
Green**
Colorless
Stand-alone enzyme Colorless
Colorless
* Amplification of >20 kb fragment sizes is possible (up to 40 kb), but may require additional optimization of reaction conditions and primer design.
** Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels.

For robust yields of templates longer than 10 kb

  Platinum SuperFi II DNA Polymerase
Amplicon size Up to 20 kb*
Fidelity vs. Taq enzyme >300x
Hot start for enhanced specificity Yes
Universal annealing temperature at 60°C Yes
GC-rich amplification Yes
Master mix format Colorless
Green**
Stand-alone enzyme Colorless
* Amplification of >20 kb fragment sizes is possible (up to 40 kb), but may require additional optimization of reaction conditions and primer design.
** Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels.

To amplify multiple targets in one reaction

  Platinum SuperFi II
DNA Polymerase

Platinum Multiplex
PCR Master Mix

No. of amplicons in single reaction Up to 15-plex Up to 20-plex
Amplicon size Up to 2.5 kb Up to 2.5 kb
Hot start for enhanced specificity Yes Yes
Fidelity vs. Taq enzyme >300x 1x
Universal annealing temperature at 60°C Yes No
GC-rich amplification Yes
No
Master mix format Colorless
Green*
Colorless
Stand-alone enzyme Colorless
 
* Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels.

Application note

To amplify DNA sequences with >65% GC content

  Platinum SuperFi II DNA Polymerase
Platinum II Taq
DNA Polymerase

Fidelity vs. Taq enzyme >300x 1x
Hot-start modification Yes Yes
Efficient amplification of >65% GC sequences Yes
Yes
Universal annealing temperature at 60°C Yes Yes
Speed 15–30 sec/kb 15 sec/kb
Amplification length Up to 20 kb Up to 5 kb
Master mix format Colorless
Green*
Colorless
Green*
Stand-alone enzyme Colorless
Colorless
* Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels.

To amplify target DNA directly from samples without DNA purification

  Platinum Direct PCR Universal Master Mix
Works across samples of various origins Yes
Universal annealing temperature at 60°C Yes
Hot-start modification Yes
Fidelity vs. Taq enzyme 1x
Hot-start modification Yes
GC-rich amplification Yes
Amplification length  Up to 8 kb*
Speed 20 sec/kb
Master mix format Green**
Stand-alone enzyme N/A
* Using the lysis protocol.
** Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels.

For accurate detection and identification of microbiome composition by 16S rRNA genes

  Platinum II Taq
DNA Polymerase

Platinum Taq
DNA Polymerase,
DNA-free

Platinum SuperFi II
DNA Polymerase

Bacterial gDNA copy per 50 μL rxn ≤1 copy ≤0.01 copy ≤1 copy
Human gDNA copy per 50 μL rxn ≤0.2 copy ≤0.001 copy ≤0.3 copy
Speed 15 sec/kb 60 sec/kb 15–30 sec/kb
Inhibitor tolerance Yes No Yes
Amplicon size Up to 5 kb Up to 5 kb Up to 20 kb
Fidelity vs. Taq enzyme 1x 1x >300x
GC-rich amplification Yes No Yes
Hot start for enhanced specificity Yes Yes Yes
Universal primer annealing Yes No Yes
Master mix format Colorless
Green*
  Colorless
Green*
Stand-alone enzyme Colorless
Colorless Colorless
* Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels

For everyday PCR amplification

  Taq DNA Polymerase
AmpliTaq DNA Polymerase
Amplicon size Up to 5 kb Up to 5 kb
Activation time Immediate Immediate
Product overhang 3′ A 3′ A
Exonuclease activity 5′→ 3′ 5′→ 3′
Master mix/SuperMix format Colorless  
Stand-alone enzyme Colorless (recombinant)
Colorless (native)*
Colorless
* Native Taq and recombinantTaq polymerase are identical in terms of their activity, specificity, thermostability, and performance in PCR. Native Taq has been purified from the host, whereas recombinant Taq has been expressed in a bacterial system and purified.

Other PCR enzymes

You can also use these enzymes for everyday PCR amplification:

To increase specificity and yield

  Platinum II Taq
Hot-Start DNA Polymerase

AmpliTaq Gold 360 DNA Polymerase
Hot-start technology Antibody Chemical
Universal annealing protocol Yes No
Speed 15 sec/kb 60 sec/kb
Flexible extension step* Yes No
Inhibitor tolerance Yes No
Enhanced specificity Yes Yes
Amplicon size Up to 5 kb Up to 5 kb
GC-rich format Yes Yes
Activation time 0.5–2 min 5–10 min
Master mix format Colorless
Green**
Colorless
Stand-alone enzyme Colorless
Green***
Colorless
* The extension step can be extended up to 60 sec/kb without the effect of specificity.
** Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels.
*** Green buffer available as separate item for use with stand-alone enzyme.

For when sequence accuracy is critical

  Platinum SuperFi II DNA Polymerase
Platinum Taq DNA Polymerase High Fidelity
Fidelity vs. Taq enzyme >300x 6x
Hot start for enhanced specificity Yes Yes
Universal annealing temperature at 60°C Yes No
Amplicon size Up to 20 kb* Up to 20 kb
Overhang Blunt 3′ A/Blunt
GC-rich amplification Yes
No
Master mix format Colorless
Green**
Colorless
Stand-alone enzyme Colorless
Colorless
* Amplification of >20 kb fragment sizes is possible (up to 40 kb), but may require additional optimization of reaction conditions and primer design.
** Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels.

For robust yields of templates longer than 10 kb

  Platinum SuperFi II DNA Polymerase
Amplicon size Up to 20 kb*
Fidelity vs. Taq enzyme >300x
Hot start for enhanced specificity Yes
Universal annealing temperature at 60°C Yes
GC-rich amplification Yes
Master mix format Colorless
Green**
Stand-alone enzyme Colorless
* Amplification of >20 kb fragment sizes is possible (up to 40 kb), but may require additional optimization of reaction conditions and primer design.
** Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels.

To amplify multiple targets in one reaction

  Platinum SuperFi II
DNA Polymerase

Platinum Multiplex
PCR Master Mix

No. of amplicons in single reaction Up to 15-plex Up to 20-plex
Amplicon size Up to 2.5 kb Up to 2.5 kb
Hot start for enhanced specificity Yes Yes
Fidelity vs. Taq enzyme >300x 1x
Universal annealing temperature at 60°C Yes No
GC-rich amplification Yes
No
Master mix format Colorless
Green*
Colorless
Stand-alone enzyme Colorless
 
* Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels.

Application note

To amplify DNA sequences with >65% GC content

  Platinum SuperFi II DNA Polymerase
Platinum II Taq
DNA Polymerase

Fidelity vs. Taq enzyme >300x 1x
Hot-start modification Yes Yes
Efficient amplification of >65% GC sequences Yes
Yes
Universal annealing temperature at 60°C Yes Yes
Speed 15–30 sec/kb 15 sec/kb
Amplification length Up to 20 kb Up to 5 kb
Master mix format Colorless
Green*
Colorless
Green*
Stand-alone enzyme Colorless
Colorless
* Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels.

To amplify target DNA directly from samples without DNA purification

  Platinum Direct PCR Universal Master Mix
Works across samples of various origins Yes
Universal annealing temperature at 60°C Yes
Hot-start modification Yes
Fidelity vs. Taq enzyme 1x
Hot-start modification Yes
GC-rich amplification Yes
Amplification length  Up to 8 kb*
Speed 20 sec/kb
Master mix format Green**
Stand-alone enzyme N/A
* Using the lysis protocol.
** Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels.

For accurate detection and identification of microbiome composition by 16S rRNA genes

  Platinum II Taq
DNA Polymerase

Platinum Taq
DNA Polymerase,
DNA-free

Platinum SuperFi II
DNA Polymerase

Bacterial gDNA copy per 50 μL rxn ≤1 copy ≤0.01 copy ≤1 copy
Human gDNA copy per 50 μL rxn ≤0.2 copy ≤0.001 copy ≤0.3 copy
Speed 15 sec/kb 60 sec/kb 15–30 sec/kb
Inhibitor tolerance Yes No Yes
Amplicon size Up to 5 kb Up to 5 kb Up to 20 kb
Fidelity vs. Taq enzyme 1x 1x >300x
GC-rich amplification Yes No Yes
Hot start for enhanced specificity Yes Yes Yes
Universal primer annealing Yes No Yes
Master mix format Colorless
Green*
  Colorless
Green*
Stand-alone enzyme Colorless
Colorless Colorless
* Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels

For everyday PCR amplification

  Taq DNA Polymerase
AmpliTaq DNA Polymerase
Amplicon size Up to 5 kb Up to 5 kb
Activation time Immediate Immediate
Product overhang 3′ A 3′ A
Exonuclease activity 5′→ 3′ 5′→ 3′
Master mix/SuperMix format Colorless  
Stand-alone enzyme Colorless (recombinant)
Colorless (native)*
Colorless
* Native Taq and recombinantTaq polymerase are identical in terms of their activity, specificity, thermostability, and performance in PCR. Native Taq has been purified from the host, whereas recombinant Taq has been expressed in a bacterial system and purified.

Other PCR enzymes

You can also use these enzymes for everyday PCR amplification:

  • Extra PCR buffers, when available, can be ordered from their respective enzyme pages provided above.
  • dNTPs are also available in a variety of concentrations and formats.

Need DNA-free DNA polymerases for microbial research or sensitive PCR-based assays? Find out more

Videos

Resources

  • PCR education
    Learn about the history of PCR, setup considerations, importance of DNA polymerase choice, common methods and applications, and troubleshooting.
  • Tm calculator
    Use our application to calculate the Tm of primers and estimates an appropriate annealing temperature when using different DNA polymerases.
  • Molecular biology webinars
    View our webinar series to learn more about molecular biology topics such as reverse transcription, PCR and cloning.
  • OEM & customized PCR solutions
    Discover customizable manufacturing solutions, product labeling, and packaging capabilities for your specific requirements.
Support
  • PCR and cDNA synthesis support center
    Find tips, troubleshooting help, and resources for your end-point PCR and cDNA applications. 
  • Product support documentation
    Search for manuals, protocols, Material Safety Data Sheets, product literature and certificates by catalog number or product name.
  • Contact us
    Email or call our Technical Application Scientists for additional questions regarding PCR enzymes and master mixes.