Chemically competent cells are calcium chloride treated to facilitate attachment of the plasmid DNA  to the competent cell membrane. The competent cell is alternatively heated in a water bath, this opens the pores of the cell membrane allowing entry of the plasmid. Chemically competent cells are the best solution for general cloning and subcloning application.

Flexible Packaging Formats

Each project has its own unique requirements, many of Invitrogen’s competent cells are offered in a variety of packaging formats. 

Options available for chemically competent cells:

  • One Shot – Transformation and recovery in the same tube.
  • MultiShot StripWell – 12 strips of 8 tubes in a 96 well format; do as many or as few reactions as needed
  • MultiShot FlexPlate – 96 well plate divisible into 12 x 8-well segments. Manual or automated applications
  • MultiShot – 96-well microarray plates fit automated format
  • Standard – Economical option

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High Efficiency Chemically Competent Cells (> 109 cfu⁄µg)

Strain Contains F' Episome T1 Resistance Available Blue-White Screening Cloning Methylated DNA
DH10B   Yes Yes Yes
DH5α   Yes Yes  
Mach1   Yes Yes  
OmniMAX 2 Yes Yes Yes Yes
Stbl2       Yes
TOP10™     Yes Yes
TOP10F'™ Yes Yes Yes Yes
ccb Survival™ 2   Yes Yes Yes


Medium Efficiency Chemically Competent Cells (108 - 109 cfu⁄µg)

Strain Contains
F' Episome
T1 Resistance
Available
Blue-White Screening Cloning Methylated DNA
DH10Bac     Yes  
DH5α Yes   Yes  
INVαF'™ Yes   Yes Yes
MC1061/P3       Yes
Stbl3       Yes
TOP10™     Yes Yes


Subcloning Efficiency Chemically Competent Cells (106 - 107 cfu⁄µg)

Strain Contains
F' Episome
T1 Resistance
Available
Blue-White Screening Cloning Methylated DNA
DH5α     Yes  
INV110 Yes Yes Yes  
TOP10/P3     Yes Yes