Invitrogen™

      One Shot™ INV110 Chemically Competent E. coli

      One Shot™ INV110 Chemically Competent <i>E. coli</i>

      Catalog number:  C717103

       Related applications: Transformation

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      Description

      INV110 E. coli are derived from JM110 E. coli and are specifically designed for the growth and purification of plasmid DNA that will be digested with dam or dcm methylation-sensitive restriction enzymes. INV110 is a significant improvement over the JM110 strain. INV110 has been engineered to carry an endA1 mutation, which eliminates the non-specific endonuclease Endonuclease I, for improved plasmid DNA preparation. In addition, the INV110 genotype features:

      • Blue/white screening of recombinant clones (lacZΔM15)
      • Reduced cleavage of foreign methylated DNA for improved transformation of genomic DNA [Δ(mcrC-mrr)]
      • F´ episome for phage infection and rescue of single-strand DNA
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      Competent Cell Type: Chemically Competent
      Species: E. coli
      Format: One Shot
      Transformation Efficiency: > 1x10^6
      Bacterial or Yeast Strain: INV110
      Product Line: One Shot®
      Blue-White Screening: Yes
      Cloning Methylated DNA: No
      Cloning Unstable DNA: Not Suitable for cloning unstable DNA
      F' Episome (to make ssDNA): Contains F' Episome
      High Throughput Compatibility: Not High Throughput-Compatible (Manual)
      Improves Plasmid Quality: Yes
      Preparing Unmethylated DNA: Suitable for preparing unmethylated DNA
      Product Size: 20 x 50 µL
      Propagating ccdB Vectors: Not for ccdB vector propagation
      Reduces Recombination: No
      T1 Phage - Resistant (tonA): Yes

      Contents & storage

      One Shot® INV110 Chemically Competent E. coli contains 10 transformations of chemically competent cells in One Shot® format. Store at -80°C.

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