Invitrogen™

One Shot™ INV110 Chemically Competent E. coli

Catalog number: C717103
Related applications: Transformation
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One Shot&trade; INV110 Chemically Competent <i>E. coli</i>
Invitrogen™

One Shot™ INV110 Chemically Competent E. coli

Catalog number: C717103
Related applications: Transformation
Technical SupportCustomer Service
Catalog Number
C717103
also known as C7171-03
Unit Size
21 x 50 µL/tube
Price (USD)
Price: 423.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
C717103
also known as C7171-03
21 x 50 µL/tube
Price: 423.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
Recommendations
INV110 E. coli are derived from JM110 E. coli and are specifically designed for the growth and purification of plasmid DNA that will be digested with dam or dcm methylation-sensitive restriction enzymes. INV110 is a significant improvement over the JM110 strain. INV110 has been engineered to carry an endA1 mutation, which eliminates the non-specific endonuclease Endonuclease I, for improved plasmid DNA preparation. In addition, the INV110 genotype features:

• Blue/white screening of recombinant clones (lacZΔM15)
• Reduced cleavage of foreign methylated DNA for improved transformation of genomic DNA [Δ(mcrC-mrr)]
• F´ episome for phage infection and rescue of single-strand DNA
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
Competent Cell
Contains F' Episome
Contains F' Episome
Efficiency
Subcloning Efficiency (10^6-10^7 cfu/µg)
Cell Type
Chemically Competent
Improves Plasmid Quality
Yes
Cloning Methylated DNA
No
Transformation Efficiency Level
Subcloning Efficiency (10^6-10^7 cfu⁄µg)
Cloning Unstable DNA
Not Suitable for cloning unstable DNA
Bacterial or Yeast Strain
INV110
Blue/White Screening
Yes
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Preparing Unmethylated DNA
Suitable for preparing unmethylated DNA
Propagating ccdB Vectors
Not for ccdB vector propagation
Reduces Recombination
No
T1 Phage - Resistant (tonA)
Yes
Species
E. coli
Format
One Shot
Product Line
One Shot™
Quantity
20 x 50 μL

Contents & Storage

One Shot™ INV110 Chemically Competent E. coli contains 10 transformations of chemically competent cells in One Shot™ format. Store at -80°C.

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