One Shot® INV110 Chemically Competent E. coli

Description

INV110 E. coliare derived from JM110 E. coliand are specifically designed for the growth and purification of plasmid DNA that will be digested with dam or dcm methylation-sensitive restriction enzymes. INV110 is a significant improvement over the JM110 strain. INV110 has been engineered to carry an endA1 mutation, which eliminates the non-specific endonuclease Endonuclease I, for improved plasmid DNA preparation. In addition, the INV110 genotype features:

• Blue/white screening of recombinant clones (lacZΔM15)
• Reduced cleavage of foreign methylated DNA for improved transformation of genomic DNA [Δ(mcrC-mrr)]
• F´ episome for phage infection and rescue of single-strand DNA

Specifications

Competent Cell Type:	Chemically Competent
Species:	E. coli
Format:	One Shot
Transformation Efficiency:	> 1x10^6
Bacterial or Yeast Strain:	INV110
Product Size:	20 x 50 µL
Blue-White Screening:	Yes
Cloning Unstable DNA:	Not Suitable for cloning unstable DNA
Reduces Recombination:	No
Cloning Methylated DNA:	No
Improves Plasmid Quality:	Yes
Propagating ccdB Vectors:	Not for ccdB vector propagation
F' Episome (to make ssDNA):	Contains F' Episome
Preparing Unmethylated DNA:	Suitable for preparing unmethylated DNA
T1 Phage - Resistant (tonA):	No
High Throughput Compatibility:	Not High Throughput-Compatible (Manual)

Contents & storage