One Shot™ INV110 Chemically Competent E. coli

Description

INV110 E. coli are derived from JM110 E. coli and are specifically designed for the growth and purification of plasmid DNA that will be digested with dam or dcm methylation-sensitive restriction enzymes. INV110 is a significant improvement over the JM110 strain. INV110 has been engineered to carry an endA1 mutation, which eliminates the non-specific endonuclease Endonuclease I, for improved plasmid DNA preparation. In addition, the INV110 genotype features:

• Blue/white screening of recombinant clones (lacZΔM15)
• Reduced cleavage of foreign methylated DNA for improved transformation of genomic DNA [Δ(mcrC-mrr)]
• F´ episome for phage infection and rescue of single-strand DNA

Specifications

Bacterial or Yeast Strain:	INV110
Blue/White Screening:	Yes
Cloning Methylated DNA:	No
Cloning Unstable DNA:	Not Suitable for cloning unstable DNA
Competent Cell Type:	Chemically Competent
F'episome (to make ssDNA):	Contains F' Episome
High-throughput Compatibility:	Not High-throughput Compatible (Manual)
Preparing Unmethylated DNA:	Suitable for preparing unmethylated DNA
Product Line:	One Shot™
Propagating ccdB Vectors:	Not for ccdB vector propagation
Reduces Recombination:	No
T1 Phage - Resistant (tonA):	Yes
Transformation Efficiency:	> 1x10^6
Transformation Efficiency Level:	Subcloning Efficiency (10^6-10^7 cfu⁄µg)
Species:	E. coli
Format:	One Shot
Improves Plasmid Quality:	Yes

Contents & storage