One Shot™ INV110 Chemically Competent E. coli

Catalog number:  C717103

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INV110 E. coli are derived from JM110 E. coli and are specifically designed for the growth and purification of plasmid DNA that will be digested with dam or dcm methylation-sensitive restriction enzymes. INV110 is a significant improvement over the JM110 strain. INV110 has been engineered to carry an endA1 mutation, which eliminates the non-specific endonuclease Endonuclease I, for improved plasmid DNA preparation. In addition, the INV110 genotype features:

• Blue/white screening of recombinant clones (lacZΔM15)
• Reduced cleavage of foreign methylated DNA for improved transformation of genomic DNA [Δ(mcrC-mrr)]
• F´ episome for phage infection and rescue of single-strand DNA
For Research Use Only. Not for use in diagnostic procedures.


Bacterial or Yeast Strain: INV110
Blue/White Screening: Yes
Cloning Methylated DNA: No
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
Competent Cell Type: Chemically Competent
F'episome (to make ssDNA): Contains F' Episome
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Preparing Unmethylated DNA: Suitable for preparing unmethylated DNA
Product Line: One Shot™
Propagating ccdB Vectors: Not for ccdB vector propagation
Reduces Recombination: No
T1 Phage - Resistant (tonA): Yes
Transformation Efficiency: > 1x10^6
Transformation Efficiency Level: Subcloning Efficiency (10^6-10^7 cfu⁄µg)
Species: E. coli
Format: One Shot
Improves Plasmid Quality: Yes

Contents & storage

One Shot™ INV110 Chemically Competent E. coli contains 10 transformations of chemically competent cells in One Shot™ format. Store at -80°C.