One Shot® INVαF´ Chemically Competent E. coli

Catalog number: C202003

Invitrogen™  Related applications: Transformation

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Description

INVαF´ E. coliallow stable replication of high-copy number plasmids. The genotype of INVαF´ offers the following features:

• Blue/white screening of recombinant clones (lacZΔM15)
• Reduction of homologous recombination of transformed plasmids (recA)
• Increased quality of plasmid DNA preparations (endA1)
• Production and rescue of single-strand DNA from vectors carrying the f1 origin of replication (F´)
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Competent Cell Type: Chemically Competent
Species: E. coli
Format: One Shot
Transformation Efficiency: > 1x10^8
Bacterial or Yeast Strain: INVαF´
Blue-White Screening: Yes
Cloning Methylated DNA: Yes
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
F' Episome (to make ssDNA): Contains F' Episome
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Improves Plasmid Quality: Yes
Plasmid Type: High Copy Plasmid
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
Product Size: 20 x 50 µL
Propagating ccdB Vectors: Not for ccdB vector propagation
Reduces Recombination: Yes
T1 Phage - Resistant (tonA): No

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