Invitrogen™

One Shot™ INVαF´ Chemically Competent E. coli

Catalog number:  C202003

Related applications:

Cloning

Back to top

Description

INVαF´ E. coliallow stable replication of high-copy number plasmids. The genotype of INVαF´ offers the following features:

• Blue/white screening of recombinant clones (lacZΔM15)
• Reduction of homologous recombination of transformed plasmids (recA)
• Increased quality of plasmid DNA preparations (endA1)
• Production and rescue of single-strand DNA from vectors carrying the f1 origin of replication (F´)
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Bacterial or Yeast Strain: INVαF´
Blue/White Screening: Yes
Cloning Methylated DNA: Yes
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
Competent Cell Type: Chemically Competent
F'episome (to make ssDNA): Contains F' Episome
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Plasmid: High Copy Plasmid
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
Product Line: One Shot™
Propagating ccdB Vectors: Not for ccdB vector propagation
Reduces Recombination: Yes
T1 Phage - Resistant (tonA): No
Transformation Efficiency: > 1x10^8
Transformation Efficiency Level: Medium Efficiency (10^8-10^9 cfu⁄µg)
Format: One Shot
Improves Plasmid Quality: Yes
Species: E. coli