Invitrogen™

One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli

Catalog number: C854003
Invitrogen™

One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli

Catalog number: C854003
Catalog Number
C854003
also known as C8540-03
Unit Size
21 x 50 µL/tube
Price (USD)
467.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
C854003
also known as C8540-03
21 x 50 µL/tube
467.00
Your Price:
***
Product Overview
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The One Shot® OmniMAX™ 2 T1R E. coli strain is an improved chemically competent cell line, perfect for use in all cloning applications, including the Gateway® technology. OmniMAX™ 2 T1R cells offer the highest transformation efficiency of any chemically competent cells in a One Shot® format, with >5 x 109 transformants/µg pUC19. They also provide efficient transformation of highly methylated DNA, since OmniMAX™ 2 T1R cells lack the E. coli K12 restriction systems (mcrA Δ(mrr hsdRMS-mcrBC)). In addition, the strain carries the tonA genotype, which confers resistance to T1 and T5 phage infection. This protects your samples and minimizes the possibility of downtime in your lab due to phage contamination. The highly versatile OmniMAX™ 2 T1R strain offers the following benefits:

• Ideal for transformation of Gateway® and TOPO® reactions
• Resistance to T1 and T5 phage (tonA)
• Construction of more representative genomic libraries due to the elimination of mcrA, mcrBC, mrr, and hsdRMS
• Blue/white screening of recombinant clones (lacZΔM15)

Genotype: F´ {proAB lacIq lacZΔM15 Tn10(TetR ) Δ(ccdAB)} mcrA Δ(mrr hsdRMS-mcrBC) Φ 80(lacZ)ΔM15 Δ(lacZYA-argF)U169 endA1 recA1 supE44 thi-1 gyrA96 relA1 tonA panD
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Bacterial or Yeast Strain
OmniMAX™2
Blue/White Screening
Yes
Cloning Unstable DNA
Not Suitable for cloning unstable DNA
For Use With (Application)
All Cloning Application
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Plasmid
May be used for plasmids > 20 kb
Preparing Unmethylated DNA
Not Suitable for preparing unmethylated DNA
Product Line
One Shot™
Propagating ccdB Vectors
Not for ccdB vector propagation
Quantity
21 x 50 μL
Reduces Recombination
Yes
Shipping Condition
Dry Ice
T1 Phage - Resistant (tonA)
Yes
Format
One Shot
Product Type
Competent Cells
Species
E. coli
Contains F' Episome
Contains F' Episome
Efficiency
> 5x10^9
Cell Type
Chemically Competent
Improves Plasmid Quality
Yes
Cloning Methylated DNA
Yes
Transformation Efficiency Level
High Efficiency (> 10^9 cfu⁄µg)

Contents & Storage

Contains:
• One Shot™ OmniMAX™ 2 T1R E. coli: 21 vials, 50 µl each
• pUC19 DNA (10 pg/µl): 1 vial, 50 µl
• S.O.C. medium: 1 bottle, 6 ml

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.

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