The One Shot™ OmniMAX™ 2 T1^R E. coli strain is an improved chemically competent cell line, perfect for use in all cloning applications, including the Gateway™ technology. OmniMAX™ 2 T1^R cells offer the highest transformation efficiency of any chemically competent cells in a One Shot™ format, with >5 x 10⁹ transformants/µg pUC19. They also provide efficient transformation of highly methylated DNA, since OmniMAX™ 2 T1^R cells lack the E. coli K12 restriction systems (mcrA Δ(mrr hsdRMS-mcrBC)). In addition, the strain carries the tonA genotype, which confers resistance to T1 and T5 phage infection. This protects your samples and minimizes the possibility of downtime in your lab due to phage contamination. The highly versatile OmniMAX™ 2 T1^R strain offers the following benefits:

• Ideal for transformation of Gateway™ and TOPO™ reactions
• Resistance to T1 and T5 phage (tonA)
• Construction of more representative genomic libraries due to the elimination of mcrA, mcrBC, mrr, and hsdRMS
• Blue/white screening of recombinant clones (lacZΔM15)

Genotype: F´ {proAB lacI^q lacZΔM15 Tn10(Tet^R ) Δ(ccdAB)} mcrA Δ(mrr hsdRMS-mcrBC) Φ 80(lacZ)ΔM15 Δ(lacZYA-argF)U169 endA1 recA1 supE44 thi-1 gyrA96 relA1 tonA panD