One Shot® OmniMAX™ 2 T1R Chemically Competent E. coli

Catalog number: C854003

Invitrogen™  Related applications: Transformation

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Description

The One Shot® OmniMAX™ 2 T1R E. coli strain is an improved chemically competent cell line, perfect for use in all cloning applications, including the Gateway® technology. OmniMAX™ 2 T1R cells offer the highest transformation efficiency of any chemically competent cells in a One Shot® format, with >5 x 109 transformants/µg pUC19. They also provide efficient transformation of highly methylated DNA, since OmniMAX™ 2 T1R cells lack the E. coli K12 restriction systems (mcrA Δ(mrr hsdRMS-mcrBC)). In addition, the strain carries the tonA genotype, which confers resistance to T1 and T5 phage infection. This protects your samples and minimizes the possibility of downtime in your lab due to phage contamination. The highly versatile OmniMAX™ 2 T1R strain offers the following benefits:

• Ideal for transformation of Gateway® and TOPO® reactions
• Resistance to T1 and T5 phage (tonA)
• Construction of more representative genomic libraries due to the elimination of mcrA, mcrBC, mrr, and hsdRMS
• Blue/white screening of recombinant clones (lacZΔM15)

Genotype: F´ {proAB lacIq lacZΔM15 Tn10(TetR ) Δ(ccdAB)} mcrA Δ(mrr hsdRMS-mcrBC) Φ 80(lacZ)ΔM15 Δ(lacZYA-argF)U169 endA1 recA1 supE44 thi-1 gyrA96 relA1 tonA panD
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Competent Cell Type: Chemically Competent
Species: E. coli
Format: One Shot
Transformation Efficiency: > 5x10^9
Bacterial or Yeast Strain: OmniMAX™2
Blue-White Screening: Yes
Cloning Methylated DNA: Yes
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
F' Episome (to make ssDNA): Contains F' Episome
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Improves Plasmid Quality: Yes
Plasmid Type: May be used for plasmids > 20 kb
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
Product Size: 20 transformations
Propagating ccdB Vectors: Not for ccdB vector propagation
Reduces Recombination: Yes
T1 Phage - Resistant (tonA): Yes
Shipping Condition: Dry Ice

Contents & storage

Contains:
• One Shot® OmniMAX™ 2 T1R E. coli: 21 vials, 50 µl each
• pUC19 DNA (10 pg/µl): 1 vial, 50 µl
• S.O.C. medium: 1 bottle, 6 ml

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.

Documents

Manuals & protocols

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