One Shot® PIR2 Chemically Competent E. coli

Catalog number: C111110

Invitrogen™  Related applications: Transformation

Error loading your content!

  Catalog number
Select a plan
Unit size
List price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

The Echo™ Cloning System is a two-vector system that utilizes the Cre-lox site-specific recombination system of bacteriophage P1 (1,2) for rapid cloning into multiple vectors. Your gene of interest is cloned into one of the specially designed donor vectors which can then be recombined with as many different Echo™ acceptor vectors as desired. The donor and acceptor vectors are placed in a buffer that contains Cre recombinase for a 25-minute, in vitro recombination reaction. Cre binds to the lox site present on each vector, brings them together, then cleaves and covalently reattaches the DNA strands. The end result is a fusion of the donor and acceptor vectors forming a single, functional expression vector.
PIR2 competent cells are recommended for maintaining constructs that express toxic genes or for libraries. Use them for cloning and maintenance of your donor vector construct (or other vector containing the R6K? origin). It contains the wild-type pir gene
for maintenance of the vector at ~15 copies per cell.
For Research Use Only. Not for use in diagnostic procedures.

Figures

Specifications

Format: One Shot
Species: E. coli
Product Size: 10 reactions
Competent Cell Type: Chemically Competent
Blue-White Screening: No
Reduces Recombination: Yes
Cloning Methylated DNA: No
Improves Plasmid Quality: Yes
Propagating ccdB Vectors: Not for ccdB vector propagation
Bacterial or Yeast Strain: PIR2
Transformation Efficiency: > 1x10^8
F' Episome (to make ssDNA): Lacks F' Episome
T1 Phage - Resistant (tonA): No
High Throughput Compatibility: Not High Throughput-Compatible (Manual)

Documents

Manuals & protocols

Recommended products