Invitrogen™

One Shot™ PIR2 Chemically Competent E. coli

Catalog number: C111110
Invitrogen™

One Shot™ PIR2 Chemically Competent E. coli

Catalog number: C111110
Catalog Number
C111110
also known as C1111-10
Unit Size
11 x 50 µL/tube
Price (USD)
305.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
C111110
also known as C1111-10
11 x 50 µL/tube
305.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
The Echo™ Cloning System is a two-vector system that utilizes the Cre-lox site-specific recombination system of bacteriophage P1 (1,2) for rapid cloning into multiple vectors. Your gene of interest is cloned into one of the specially designed donor vectors which can then be recombined with as many different Echo™ acceptor vectors as desired. The donor and acceptor vectors are placed in a buffer that contains Cre recombinase for a 25-minute, in vitro recombination reaction. Cre binds to the lox site present on each vector, brings them together, then cleaves and covalently reattaches the DNA strands. The end result is a fusion of the donor and acceptor vectors forming a single, functional expression vector.
PIR2 competent cells are recommended for maintaining constructs that express toxic genes or for libraries. Use them for cloning and maintenance of your donor vector construct (or other vector containing the R6K? origin). It contains the wild-type pir gene
for maintenance of the vector at ∼15 copies per cell.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Bacterial or Yeast Strain
PIR2
Blue/White Screening
No
Cloning Methylated DNA
No
Competent Cell Type
Chemically Competent
F'episome (to make ssDNA)
Lacks F' Episome
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Propagating ccdB Vectors
Not for ccdB vector propagation
Reduces Recombination
Yes
T1 Phage - Resistant (tonA)
No
Transformation Efficiency
> 1x10^8
Format
One Shot
Product Line
One Shot™
Product Type
Competent Cells
Species
E. coli
Improves Plasmid Quality
Yes

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Frequently asked questions (FAQs)

Citations & References