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Invitrogen™
MAX Efficiency™ Stbl2™ Competent Cells
Catalog number: 10268019
Invitrogen™
MAX Efficiency™ Stbl2™ Competent Cells
Catalog number: 10268019
Catalog Number
10268019
also known as 10268-019
Unit Size
5 x 200 µL
Price (USD)
Price: 334.00
Your Price:
Availability
-
Quantity
| Catalog Number | Unit Size | Price (USD) | Availability | Quantity | |
|---|---|---|---|---|---|
| 10268019 also known as 10268-019 | 5 x 200 µL | Price: 334.00 Your Price: | - |
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Product Overview
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MAX Efficiency™ Stbl2™ Competent Cells are high-efficiency chemically competent cells specifically designed for cloning unstable inserts. In addition to recA1, a unique set of genetic markers allow for stable cloning of direct repeat and retroviral sequences and tandem array genes. MAX Efficiency™ Stbl2™ Competent Cells offer:
• >1 x 109 transformants/µg plasmid DNA for cloning rare sequences
• Elimination of mcrBC-hsdRMS-mrr and mutation of mcrA—allows cloning of methylated genomic sequences
• A unique genotype that stabilizes direct repeat and retroviral sequences such as SIV and HIV
• Greatly increased plasmid yield and quality due to endA1 mutation
• >1 x 109 transformants/µg plasmid DNA for cloning rare sequences
• Elimination of mcrBC-hsdRMS-mrr and mutation of mcrA—allows cloning of methylated genomic sequences
• A unique genotype that stabilizes direct repeat and retroviral sequences such as SIV and HIV
• Greatly increased plasmid yield and quality due to endA1 mutation
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product Type
Competent Cell
Contains F' Episome
Lacks F' Episome
Efficiency
High Efficiency (>10^9 cfu/µg)
Cell Type
Chemically Competent
Improves Plasmid Quality
Yes
Cloning Methylated DNA
Yes
Transformation Efficiency Level
High Efficiency (> 10^9 cfu⁄µg)
Cloning Unstable DNA
Suitable for cloning unstable DNA
Bacterial or Yeast Strain
Stbl2™
Blue/White Screening
No
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Plasmid
May be used for plasmids > 20 kb
Preparing Unmethylated DNA
Not Suitable for preparing unmethylated DNA
Propagating ccdB Vectors
Not for ccdB vector propagation
Reduces Recombination
Yes
Shipping Condition
Dry Ice
T1 Phage - Resistant (tonA)
No
Species
E. coli
Format
Tube
Product Line
MAX Efficiency™, Stbl2™
Quantity
5 x 200 μL
Contents & Storage
Contains:
• MAX Efficiency™ Stbl2™ Competent Cells: 5 vials, 200 µl each (total of 1 ml)
• pUC19 DNA (0.01 µg/ml): 1 vial, 100 µl
• SOC Medium: 1 bottle, 6 ml
Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.
• MAX Efficiency™ Stbl2™ Competent Cells: 5 vials, 200 µl each (total of 1 ml)
• pUC19 DNA (0.01 µg/ml): 1 vial, 100 µl
• SOC Medium: 1 bottle, 6 ml
Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.