Invitrogen™

MAX Efficiency™ Stbl2™ Competent Cells

Catalog number:  10268019

Related applications:

Cloning

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Description

MAX Efficiency® Stbl2™ Competent Cells are high-efficiency chemically competent cells specifically designed for cloning unstable inserts. In addition to recA1, a unique set of genetic markers allow for stable cloning of direct repeat and retroviral sequences and tandem array genes. MAX Efficiency® Stbl2™ Competent Cells offer:

• >1 x 109 transformants/µg plasmid DNA for cloning rare sequences
• Elimination of mcrBC-hsdRMS-mrr and mutation of mcrA—allows cloning of methylated genomic sequences
• A unique genotype that stabilizes direct repeat and retroviral sequences such as SIV and HIV
• Greatly increased plasmid yield and quality due to endA1 mutation
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Bacterial or Yeast Strain: Stbl2™
Blue/White Screening: No
Cloning Methylated DNA: Yes
Cloning Unstable DNA: Suitable for cloning unstable DNA
Competent Cell Type: Chemically Competent
F'episome (to make ssDNA): Lacks F' Episome
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Plasmid: May be used for plasmids > 20 kb
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
Product Line: MAX Efficiency™, Stbl2™
Propagating ccdB Vectors: Not for ccdB vector propagation
Reduces Recombination: Yes
Shipping Condition: Dry Ice
T1 Phage - Resistant (tonA): No
Transformation Efficiency: > 1x10^9
Transformation Efficiency Level: High Efficiency (> 10^9 cfu⁄µg)
Format: Tube(s)
Improves Plasmid Quality: Yes
Species: E. coli

Contents & storage

Contains:
• MAX Efficiency® Stbl2™ Competent Cells: 5 vials, 200 µl each (total of 1 ml)
• pUC19 DNA (0.01 µg/ml): 1 vial, 100 µl
• SOC Medium: 1 bottle, 6 ml

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.