- Rapid loading (typically 15 min to 1 hr)
- Short-term tracking (typically 3–6 generations)
- Moderate resistance to photobleaching
- Moderate brightness
- Signal distribution throughout the cell
The simplest processes for monitoring cell movement and location use tracking probes that pass through the membrane into the cell and become membrane-impermeant after loading. Ideal tracking dyes are passed to daughter cells for multiple generations but do not transfer to neighboring cells on contact. Invitrogen CellTracker dyes exhibit ideal properties—they are stable, nontoxic at working concentrations, well retained in cells, and brightly fluorescent at physiological pH. CellTracker dyes have a wide range of emission spectra for easy combination with antibody staining.