CellTracker Orange CMRA is a fluorescent dye well suited for monitoring cell movement or location. After loading into cells, the dye is well retained, allowing for multigenerational tracking of cellular movements. The orange excitation/emission spectra are ideal for multiplexing with green fluorescent dyes and proteins.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

• Easy to use—remove medium, add dye, incubate 30 minutes, and image cells
• Fluorescent signal retention of >72 hours (typically three to six generations)
• Orange excitation/emission spectra (548/576 nm maxima) ideal for multiplexing
• Low cytotoxicity—does not affect viability or proliferation

CellTracker Orange CMRA fluorescent dye has been designed to freely pass through cell membranes into cells, where it is transformed into a cell-impermeant, fluorescent dye. CellTracker Orange CMRA dye is retained in living cells through several generations. The dye is transferred to daughter but not adjacent cells in a population. CellTracker Orange CMRA dye is designed to display fluorescence for at least 72 hours, and the dye exhibits ideal tracking properties: it is stable, nontoxic at working concentrations, well retained in cells, and brightly fluorescent at physiological pH. Additionally, the excitation and emission spectra of CellTracker Orange CMRA dye are well separated from GFP (green fluorescent protein) spectra allowing for multiplexing.

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