Chemically competent cells are calcium chloride treated to facilitate attachment of the plasmid DNA  to the competent cell membrane. The competent cell is alternatively heated in a water bath, this opens the pores of the cell membrane allowing entry of the plasmid. Chemically competent cells are the best solution for general cloning and subcloning application.

Flexible Packaging Formats

Each project has its own unique requirements, many of Invitrogen’s competent cells are offered in a variety of packaging formats. 

Options available for chemically competent cells:

  • One Shot – Transformation and recovery in the same tube.
  • MultiShot StripWell – 12 strips of 8 tubes in a 96 well format; do as many or as few reactions as needed
  • MultiShot FlexPlate – 96 well plate divisible into 12 x 8-well segments. Manual or automated applications
  • MultiShot – 96-well microarray plates fit automated format
  • Standard – Economical option

Learn more

High Efficiency Chemically Competent Cells (> 109 cfu⁄µg)

StrainContains F' EpisomeT1 Resistance AvailableBlue-White ScreeningCloning Methylated DNA
DH10B YesYesYes
DH5α YesYes 
Mach1 YesYes 
OmniMAX 2YesYesYesYes
Stbl2   Yes
TOP10™  YesYes
TOP10F'™YesYesYesYes
ccb Survival™ 2 YesYesYes


Medium Efficiency Chemically Competent Cells (108 - 109 cfu⁄µg)

StrainContains
F' Episome
T1 Resistance
Available
Blue-White ScreeningCloning Methylated DNA
DH10Bac  Yes 
DH5αYes Yes 
INVαF'™Yes YesYes
MC1061/P3   Yes
Stbl3   Yes
TOP10™  YesYes


Subcloning Efficiency Chemically Competent Cells (106 - 107 cfu⁄µg)

StrainContains
F' Episome
T1 Resistance
Available
Blue-White ScreeningCloning Methylated DNA
DH5α  Yes 
INV110YesYesYes 
TOP10/P3  YesYes