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Whether you’re PCR cloning with Taq DNA polymerase or a proofreading enzyme, there is a TOPO® cloning kit available to take you quickly and efficiently into your downstream application.

 

Application of cloned PCR product Product Type of TOPO® cloning Advantages One Shot® Competent Cells Cat. no.  
General subcloningTOPO® TA Cloning® KitTA
  • Get your clone 95% efficiency
  • Selection of competent cells for high speed, electroporation, or routine cloning

    • NEW

    TOPO® TA Cloning® Kit for Subcloning without Competent Cells

    		450641Order Now
    Mach1-T1RK451020Order Now
    TOP10K450001Order Now
    TOP10F’K455001Order Now
    TOP10 Electrocomp™K456001 Order Now
    MAX Efficiency® DH5α™-T1 Phage-ResistantK452001Order Now
    Zero Blunt® TOPO® PCR Cloning KitBlunt
  • Unique technology minimizes background
  • Get your clone 95% efficiency
  • Multiple primer sites (T7, T3, M13F, M13R) makes sequence analysis easy and convinient
  • Selection of competent cells for high speed, electroporation, or routine cloning

    • NEW

    Zero Blunt® TOPO® PCR Cloning Kit without Competent Cells

    		450245Order Now
    Mach1™-T1RK283020Order Now
    TOP10K280020 Order Now
    TOP10 Electrocomp™K286020 Order Now
    MAX Efficiency® DH5α™-T1 Phage-ResistantK282020 Order Now
    Long PCR fragmentsTOPO® XL PCR Cloning KitTA
  • Combining TOPO®, ZeroBackground™ and Crystal Violet technologies makes for easy cloning of large fragments 3-10 kb.
  • Unique technology to minimize background
    		• Mach1™-T1RK703020Order Now
    TOP10K475010 Order Now
    TOP10 Electrocomp™K470010 Order Now
    In vitro transcriptionTOPO® TA Cloning® Kit Dual PromoterTA
  • Get your clone 95% efficiency
  • Dual T7 and SP6 priming sites for in vitro transcription
    		• Mach1™-T1RK461020 Order Now
    TOP10F’K460001Order Now
    TOP10 Electrocomp™K466001 Order Now
    MAX Efficiency® DH5α™ -T1 Phage-ResistantK462001 Order Now
    SequencingTOPO® TA Cloning® Kit for Sequencing  (pCR® 4 TOPO® Vector)TA
  • Get your clone 95% efficiency
  • Sequence more insert, less vector
  • Multiple primer sites (T7, T3, M13F, M13R) makes sequence analysis easy and convinient 

    • NEW

    TOPO® TA Cloning® Kit for Sequencing without Competent Cells

    		450030Order Now
    Mach1™-T1RK453020Order Now
    TOP10K457501 Order Now
    TOP10 Electrocomp™K458001 Order Now
    MAX Efficiency® DH5α™ -T1 Phage-ResistantK289520 Order Now
    Zero Blunt® TOPO® PCR Cloning Kit for Sequencing (pCR® 4 Blunt TOPO® Vector)Blunt
  • Unique technology minimizes background
  • Get your clone 95% efficiency
  • Sequence more insert, less vector
    		• Mach1™-T1RK283520 Order Now
    TOP10K287520 Order Now
    MAX Efficiency® DH5α™-T1 Phage-ResistantK289520 Order Now
    TOP10 Electrocomp™K288020 Order Now
    Expression in E. coliChampion™ pET100 Series Directional TOPO® Expression Kits (pET Directional TOPO® Vector)Directional
  • Up to 90% of clones in the correct orientation
  • High-level protein expression using E. coli
  • Convenient purification and detection tags
    		• BL21 Star™(DE3)K10001 Order Now
    K10101 Order Now
    K10201 Order Now
    K15101 Order Now
    Expression in mammalian cellspcDNA™ 3.3 TOPO® TA Cloning® KitTA
  • 3–5 higher expression compared to conventional CMV vectors
  • Ideal for expression of native protein
    		• TOP10K830001 Order Now
    pcDNA™ 3.2/V5/GW/D-TOPO® Cloning KitDirectional
  • Greater than 90% of clones in correct orientation
  • CMV promoter delivers high-level expression in mammalian cells
  • C-terminal V5 and 6xHis tags for convenient detection and purification
  • Neomycin selection
    		• TOP10K244020 Order Now
    ViraPower™ HiPerform™ Lentiviral FastTiter™ TOPO® Expression Kits (pLenti 7.3 Vector)TA
  • Greater than 4X higher expression compared to the original system
  • Efficient TOPO® cloning
    		• Stbl3™K495000 Order Now
    Entry into Gateway® Entry VectorspCR®8/GW/TOPO® TA Cloning® KitTA
  • Includes Plasmid miniprep kit
  • Fast growing cells for fast miniprep
    		• Mach1™-T1RK532000 Order Now
    TA
  • Fast growing cells for fast miniprep
    		• Mach1™-T1RK252020 Order Now
      TOP10K250020 Order Now
    pENTR™/TEV/DTOPO® Cloning KitDirectional
  • Fast growing cells for fast miniprep
    		• Mach1™-T1RK252520 Order Now
    pENTR™/D-TOPO® Cloning KitDirectional TOP10K240020 Order Now
    pENTR™/SD/DTOPO® Cloning KitDirectional TOP10K242020 Order Now

    For Research Use Only. Not for use in diagnostic procedures.