Search Thermo Fisher Scientific
Invitrogen™
pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1R E. coli
The pCR™8/GW/TOPO™ TA Cloning Kit delivers fast, efficient and reliable cloning. Additionally, this vector is ideal for use in Gateway™ Read more
Catalog number K252020
Price (USD)/ 20 reactions
610.00
-
Price (USD)/ 20 reactions
610.00
pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1R E. coli
Catalog numberK252020
Price (USD)/ 20 reactions
610.00
-
The pCR™8/GW/TOPO™ TA Cloning Kit delivers fast, efficient and reliable cloning. Additionally, this vector is ideal for use in Gateway™ recombination cloning into various destination vectors for a multitude of applications.
This pCR™8 cloning kit offers the following features:
• Fast, 5-minute TOPO™ TA Cloning delivers 95% recombinants
• 3´-T overhangs simplify cloning of Taq-amplified PCR products
• Fast growing MachI cells shorten cloning time
• Compatible with Gateway™ recombination cloning enabling access to a multitude of destination vectors
• Sequencing primer sites located close to cloning insertion site so you sequence more insert, less vector the first time
• Spectinomycin resistance gene for robust selection in E. coli
• EcoR I sites flanking the PCR product insertion site for easy excision of inserts
This kit includes One Shot™ Mach1™-T1R Chemically Competent E. coli.
This pCR™8 cloning kit offers the following features:
• Fast, 5-minute TOPO™ TA Cloning delivers 95% recombinants
• 3´-T overhangs simplify cloning of Taq-amplified PCR products
• Fast growing MachI cells shorten cloning time
• Compatible with Gateway™ recombination cloning enabling access to a multitude of destination vectors
• Sequencing primer sites located close to cloning insertion site so you sequence more insert, less vector the first time
• Spectinomycin resistance gene for robust selection in E. coli
• EcoR I sites flanking the PCR product insertion site for easy excision of inserts
This kit includes One Shot™ Mach1™-T1R Chemically Competent E. coli.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cloning MethodTOPO™ with Gateway™
No. of Reactions20 reactions
Product LineOne Shot™
Product TypeTA Cloning Kit
VectorpCR
Unit Size20 reactions
Contents & Storage
The pCR™8/GW/TOPO™ TA Cloning Kit contains the TOPO™-adapted, ready-to-use pCR™8/GW/TOPO™ Vector, dNTPs, Salt Solution, Sterile Water, Gateway Forward and Reverse Sequencing Primers, Control Template and PCR Primers, One Shot™ Mach1™-T1R Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid. Store the One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored