TOPO™ TA Cloning™ Kits are designed for cloning PCR products directly from a PCR reaction in just 5 minutes (1). They use a pCR™-TOPO™ Vector with covalently bound topoisomerase I for fast cloning and recombinants. pCR™-TOPO™ Vectors include:
• 3´-T overhangs for direct ligation of Taq-amplified PCR products • Choice of T7 (pCR™2.1-TOPO™) or T7 and SP6 (pCR™II-TOPO™) promoters for in vitro RNA transcription and sequencing. Each vector also contains M13 forward and reverse primer sites for sequencing. • EcoR I sites flanking the PCR product insertion site for easy excision of inserts • Kanamycin and ampicillin resistance genes for your choice of selection in E. coli • Easy blue/white colony screening for selection of recombinants
TOPO™ TA Cloning™ Kits are also available in combo kits combined with a PureLink™ Quick Plasmid Miniprep Kit (50 preps) for fast plasmid purification of your TOPO™-cloned inserts for downstream analysis.
For Research Use Only. Not for use in diagnostic procedures.
Bacterial or Yeast Strain
TOPO-TA Cloning Vectors
Contents & Storage
Box 1: • Topoisomerase I-activated pCR™2.1-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water
Store at -5 to -30°C. All reagents are stable for 6 months when properly stored.
Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid