Dynabeads FlowComp Human CD3 Kit product photo

Positive cell isolation directly from human whole blood, buffy coat, or mouse/human mononuclear cells

Flow cytometry and fluorescent-activated cell sorting (FACS) heavily rely on the user working with intact cells. Flow researchers must balance removal of cell debris and unwanted cells while preserving the integrity of cells-of-interest. Dynabeads FlowComp positive cell isolation kits positively isolate cells of interest directly followed by cell release and removal of magnetic beads. No sample preparation (e.g., density gradient centrifugation or lysis of red blood cells) is required, which helps eliminate the risk of contaminating and incubating your cells with iron, dextran, or other potentially harmful chemicals that are present in lysis buffers.

Get flow-ready cells in just three steps: 

  1. Add FlowComp antibody to sample for binding of target T cells
  2. Add magnetic beads to capture targeted T cells
  3. T cells are released from magnetic beads—ready for flow or any other downstream application

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Human icon 

Dynabeads FlowComp positive isolation kits for human cell markers CD3, CD4, CD8, and CD14

 Dynabeads magnetic beads combine with T cell markers to positively isolate specific T cell populations of interest. FlowComp positive isolation kits are available against four different human T cell markers.

  • CD3: targets cytotoxic T cells (CD8+ naive T cells) and T helper cells (CD4+ naive T cells)
  • CD4: targets T helper (Th) cells, regulatory T (Treg) cells, and natural killer T (NKT) cells
  • CD8: targets cytotoxic T cells (CTLs)
  • CD14: targets naive CD4+ T cells

Cells isolated with Dynabeads FlowComp kits keep their functional characteristics and are directly suitable for flow cytometry and any downstream cell-based assay.

View FlowComp protocol for isolation from human whole blood
Order Dynabeads FlowComp kits for human T cell markers

Mouse icon 

Dynabeads FlowComp positive isolation kits for mouse cell markers CD4, CD8, CD4/CD25, and pan T

Dynabeads magnetic beads combine with T cell markers to positively isolate your specific T cell populations of interest for your mouse studies. FlowComp positive isolation kits are available against four different mouse T cell markers.

  • CD4: targets naive CD4+ T cells
  • CD8: targets cytotoxic T cells (CD8+ naive T cells)
  • CD4/CD25: targets regulatory CD4+ T cells
  • Pan T cell: targets all T cells

Cells isolated with Dynabeads FlowComp kits are significantly purer and more viable than isolation with a column-based method (Figure 1). These cells keep their functional characteristics and are suitable to be used directly for flow cytometry and any downstream cell-based assay.


Dynabeads FlowComp build-your-own isolation kit

Combine Dynabeads magnetic beads with your antibody of choice to isolate any cell type. The Dynabeads FlowComp Flexi Kit has a wide range of species reactivity. Starting samples are compatible with the build-your-own kit:

  • Whole blood
  • Peripheral blood mononuclear cells/mononuclear cells (PBMC/MNC)
  • Single cell suspensions from lymph nodes, spleen, or other tissues

Order Dynabeads FlowComp Flexi Kit


Dynabeads FlowComp kit ordering information
Human icon 

Compatible antibodies with FlowComp Dynabeads human CD4 positive isolation kit

Mouse icon 

Compatible antibodies with FlowComp Dynabeads mouse CD4 positive isolation kit

Human icon 

Compatible antibodies with FlowComp Dynabeads human CD8 positive isolation kit

For information about tested clones from other suppliers than Thermo Fisher, please contact technical support.

For Research Use Only. Not for use in diagnostic procedures.

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