|Tested species reactivity||Human|
|Published species reactivity||Human, Rhesus monkey|
|Host / Isotype||Mouse / IgG2a|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The TRI-COLOR® conjugate is a tandem R-phycoerythrin (PE)-Cy5® conjugate that permits simultaneous multicolor labeling and detection of multiple targets with excitation by a single excitation source-the 488 nm line of the argon-ion laser.
CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35 % of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells. CD38 (T10) is a single chain 46 kDa type II integral glycoprotein with a short N terminal cytoplasmic tail. CD38 is highly expressed on thymocytes. It is also expressed by early cells of B and T lineages, NK cells, plasma cells, monocytes and macrophages and may be detected on cells from multiple myeloma, ALL (B and T) and some AML. In normal lymph nodes and tonsils the antigen is detected mainly on B cells in germinal centers and in plasma cells. The extracellular domain of the molecule shares a high homology sequence with Aplysia ADP ribosyl cyclase. CD38 functions as a multicatalytic ectoenzyme serving as ADP ribosyl cyclase, ADPR hydrolase and possibly NAD+ glycohydrolase, or as a surface receptor.
Analyte Specific Reagent
Intermediate DNA methylation is a conserved signature of genome regulation.
MHCD0806 was used in flow cytometry to show that intermediate DNA methylation is a conserved signature of gene regulation and exon usage
|Elliott G,Hong C,Xing X,Zhou X,Li D,Coarfa C,Bell RJ,Maire CL,Ligon KL,Sigaroudinia M,Gascard P,Tlsty TD,Harris RA,Schalkwyk LC,Bilenky M,Mill J,Farnham PJ,Kellis M,Marra MA,Milosavljevic A,Hirst M,Stormo GD,Wang T,Costello JF||Nature communications (6:null)||2015|
Analysis of T-cell clones in systemic lupus erythematosus.
MHCD0806 was used in flow cytometry to investigate the role Th cells and their relationship with Th2 cells in human systemic lupus erythematosus.
|Converso M,Bertero MT,Vallario A,Caligaris-Cappio F||Haematologica (85:118)||2000|
Cytokine signatures of innate and adaptive immunity in 17DD yellow fever vaccinated children and its association with the level of neutralizing antibody.
MHCD0806 was used in flow cytometry to discuss the live attenuated yellow fever vaccines.
|Luiza-Silva M,Campi-Azevedo AC,Batista MA,Martins MA,Avelar RS,da Silveira Lemos D,Bastos Camacho LA,de Menezes Martins R,de Lourdes de Sousa Maia M,Guedes Farias RH,da Silva Freire M,Galler R,Homma A,Leite Ribeiro JG,Campos Lemos JA,Auxiliadora-Martins M,Eloi-Santos SM,Teixeira-Carvalho A,Martins-Filho OA||The Journal of infectious diseases (204:873)||2011|
Reduced cell surface expression of CCR5 in CCR5Delta 32 heterozygotes is mediated by gene dosage, rather than by receptor sequestration.
MHCD0806 was used in flow cytometry to determine how CCR5 expression is reduced in cells from ccr5Delta32 heterozygotes.
|Venkatesan S,Petrovic A,Van Ryk DI,Locati M,Weissman D,Murphy PM||The Journal of biological chemistry (277:2287)||2002|
A membrane-proximal basic domain and cysteine cluster in the C-terminal tail of CCR5 constitute a bipartite motif critical for cell surface expression.
MHCD0806 was used in flow cytometry to characterize CCR5 truncations.
|Venkatesan S,Petrovic A,Locati M,Kim YO,Weissman D,Murphy PM||The Journal of biological chemistry (276:40133)||2001|
|Rhesus monkey||Not Cited||
Cytokine expression, natural killer cell activation, and phenotypic changes in lymphoid cells from rhesus macaques during acute infection with pathogenic simian immunodeficiency virus.
MHCD0806 was used in flow cytometry to examine the innate and adaptive immune responses of rhesus macaques infected with SIVmac251.
|Giavedoni LD,Velasquillo MC,Parodi LM,Hubbard GB,Hodara VL||Journal of virology (74:1648)||2000|